Enhanced expression of ncc1 and clc2c in the kidney and urinary bladder accompanies freshwater acclimation in Mozambique tilapia

Breves, Jason P.; Nelson, Nastasia; Koltenyuk, Victor; Petro-Sakuma, Cody; Celino-Brady, Fritzie T.; Seale, André P.

doi:10.1016/j.cbpa.2021.111021

Cited by 5 publications

(5 citation statements)

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“…In teleost fish, there is evidence for coupled NaCl uptake mediated by an apical Na + , Cl − cotransporter (NCC2 [or NCC-like], SLC12A10 in gills; NCC1, SLC12A3 in kidney), providing support for Model 3 ( Figure 1C ) ( Hiroi et al, 2008 ; Wang et al, 2009 ; Blondeau-Bidet et al, 2019 ; Breves et al, 2021 ). This mechanism is contrary to earlier assumptions of independent Na + and Cl − transport across fish gills ( Krogh, 1938 ).…”

Section: Ion Uptake In Teleost Fishmentioning

confidence: 91%

Mechanisms of Na+ uptake from freshwater habitats in animals

2022

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Life in fresh water is osmotically and energetically challenging for living organisms, requiring increases in ion uptake from dilute environments. However, mechanisms of ion uptake from freshwater environments are still poorly understood and controversial, especially in arthropods, for which several hypothetical models have been proposed based on incomplete data. One compelling model involves the proton pump V-type H+ ATPase (VHA), which energizes the apical membrane, enabling the uptake of Na+ (and other cations) via an unknown Na+ transporter (referred to as the “Wieczorek Exchanger” in insects). What evidence exists for this model of ion uptake and what is this mystery exchanger or channel that cooperates with VHA? We present results from studies that explore this question in crustaceans, insects, and teleost fish. We argue that the Na+/H+ antiporter (NHA) is a likely candidate for the Wieczorek Exchanger in many crustaceans and insects; although, there is no evidence that this is the case for fish. NHA was discovered relatively recently in animals and its functions have not been well characterized. Teleost fish exhibit redundancy of Na+ uptake pathways at the gill level, performed by different ion transporter paralogs in diverse cell types, apparently enabling tolerance of low environmental salinity and various pH levels. We argue that much more research is needed on overall mechanisms of ion uptake from freshwater habitats, especially on NHA and other potential Wieczorek Exchangers. Such insights gained would contribute greatly to our general understanding of ionic regulation in diverse species across habitats.

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Section: Ion Uptake In Teleost Fishmentioning

confidence: 91%

Mechanisms of Na+ uptake from freshwater habitats in animals

2022

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“…The expression of slc12a2 in the gills of silver catfish was higher at pH 5.5 compared to the other treatments, and its expression in the kidney was not significantly affected by the pH change. The occurrence of the Na + /K + /2Cl − symporter has been demonstrated, not only in the branchial and opercular epithelia [44,48,[76][77][78], but also in the renal [79] and intestinal epithelia [80,81]. Bumetamide, an inhibitor of this symporter, did not affect Na + influx in native cardinal tetra (Paracheirodon axelrodi), hemigrammus tetra (Hemigrammus rhodostomus), and moenkhausia tetra (Moenkhausia diktyota) from the Rio Negro, an acidic, ion-poor, and black water river, but these species have different Na + uptake mechanisms from the "standard" FW teleosts [82].…”

Section: Discussionmentioning

confidence: 99%

“…Other studies showed the possible involvement of some genes of the same transporters in the gills for the ionoregulation of killifish (Fundulus heteroclitus), such as slc12a10 (ncc2), slc12a1 (nkcc2), and slc12a2 (nkcc1) [11,39,44,48]. Changes in the activity of proteins and in the expression of the genes involved in the osmoregulation of freshwater teleosts illustrate some of the possible responses that animals may exhibit in the face of osmotic alterations.…”

Section: Discussionmentioning

confidence: 99%

“…Several transporters, such as atp1a1, slc12a4, slc9a3, and slc12a2, were also expressed in the gills of freshwater-adapted Mozambique tilapia (Oreochromis mossambicus), as well as slc12a10.2 (ncc) [8,[39][40][41][42][43][44]. In the gills of zebrafish, the expression of the transporters atp1a1a (nka1a), atp2b2 (pmca2), atp6v1aa rhcg1, slc4a4b (nbc), slc12a10.2 (ncc), slc12a2 (nkcc1) (low expression), slc8a (ncx), and slc9a3 (nhe3) has been identified [45,46].…”

Section: Discussionmentioning

confidence: 99%

“…All of the gene names followed the ZFIN Zebrafish Nomenclature Conventions and were named after the mammalian orthologues. These genes were chosen because their complete sequences were available in the mass sequence of silver catfish and were expressed in other freshwater-adapted teleosts [1,3,8,11,[39][40][41][42][43][44][45][46][47][48]. In addition, previous analysis with the respective primers tested indicated the expression of these genes in the gills and kidney of silver catfish.…”

Section: Expression Of Genes Related To Ion and Ammonia Transportmentioning

confidence: 99%

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Expression of Ion Transporters and Na+/K+-ATPase and H+-ATPase Activities in the Gills and Kidney of Silver Catfish (Rhamdia quelen) Exposed to Different pHs

Marx

Souza

Almeida

et al. 2022

Fishes

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Exposure to acidic and alkaline pHs results in an ionic imbalance. Cellular responses involved in osmoregulation in silver catfish exposed to different pHs (5.5, 7.5, and 9.0) for 24 h were evaluated. The gills and kidney were collected to measure Na+/K+-ATPase (NKA) and H+-ATPase (V-ATPase) activities and to evaluate the expression of ion transporter-related genes: NKA (atp1a1), H+-ATPases (atp6v0a1b, atp6v0a2a, atp6v0a2b), Na+/H+ antiporter (slc9a3), K+/Cl− symporters (slc12a4, slc12a6, slc12a7a, slc12a7b), Na+/K+/2Cl− symporter (slc12a2), and ammonium transporter Rh type b (rhbg). The gills presented greater responses to pH changes than the kidney. The pH alterations changed the atp1a1 gene expression and NKA activity, whereas the H+-ATPase activity increased in the gills in alkaline water, probably to maintain ionic balance. The slc9a3 and slc12a2 genes play more prominent roles in the ion uptake at acidic pH than H+-ATPase. The slc12a7a was the only isoform of this transporter affected by pH. The rhbg is apparently related to ammonia excretion through the gills and kidney (minor scale). Exposure to alkaline pH seems to be battled by impairment of NKA and H+-ATPase activities in the gills, whereas the expression of some ion transporters in silver catfish changes during both acidic and alkaline pHs.

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Endocrine control of gill ionocyte function in euryhaline fishes

Breves,

Shaughnessy

2024

J Comp Physiol B

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Enhanced expression of ncc1 and clc2c in the kidney and urinary bladder accompanies freshwater acclimation in Mozambique tilapia

Cited by 5 publications

References 50 publications

Mechanisms of Na+ uptake from freshwater habitats in animals

Mechanisms of Na+ uptake from freshwater habitats in animals

Expression of Ion Transporters and Na+/K+-ATPase and H+-ATPase Activities in the Gills and Kidney of Silver Catfish (Rhamdia quelen) Exposed to Different pHs

Endocrine control of gill ionocyte function in euryhaline fishes

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