Enhanced Expression of Rat Obese (ob) Gene in Adipose Tissues of Ventromedial Hypothalamus-Lesioned Rats

Funahashi, Tohru; Shimomura, Iichiro; Hiraoka, Haruo; Arai, T.; Takahashi, Motomitsu; Nakamura, Tadashi; Nozaki, Satoshi; Yamashita, Shizuya; Takemura, Koichi; Tokunaga, Katsuto; Matsuzawa, Yūji

doi:10.1006/bbrc.1995.1837

Cited by 128 publications

(62 citation statements)

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“…Leptin appears to function as an adipostat signal, communicating the status of fat stores to the brain. Recent studies have demonstrated that leptin gene expression and circulating protein levels range over many orders of magnitude, correlating closely with increased or decreased total body fat stores (39)(40)(41)(42)(43). Administration of recombinant leptin to ob/ob mice results in decreased food intake and increased energy expenditure (44)(45)(46), the later effect being mediated at least in part by sympathetic stimulation of brown adipose tissue (47).…”

Section: Brown Fat and Regulation Of Total Body Fat Storesmentioning

confidence: 99%

BROWN ADIPOSE TISSUE, Β3-Adrenergic RECEPTORS, AND OBESITY

1997

Annu. Rev. Med.

215

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Brown adipose tissue is distinguished by its unique capacity for uncoupled mitochondrial respiration, which is highly regulated by sympathetic nerve activity. Because of this, energy expenditure in brown fat is capable of ranging over many orders of magnitude. The fact that the function of brown adipose tissue is impaired in obese rodents and that transgenic mice with decreased brown fat develop obesity demonstrates the importance of brown fat in maintaining nutritional homeostasis. However, the role of brown fat in humans is less clear. β3-Adrenergic receptors are found on brown adipocytes, and treatment with β3-selective agonists markedly increases energy expenditure and decreases obesity in rodents. Whether β3-selective agonists will be effective anti-obesity agents in humans is presently under investigation.

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Section: Brown Fat and Regulation Of Total Body Fat Storesmentioning

confidence: 99%

BROWN ADIPOSE TISSUE, Β3-Adrenergic RECEPTORS, AND OBESITY

1997

Annu. Rev. Med.

215

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“…There have been several reports on complementary DNA (cDNA) cloning of leptin in various species [8,13,15,19,23], and we also reported cDNA cloning of canine leptin and the activity of its recombinant protein [14]. In this study, we cloned feline leptin cDNA using reverse transcription-polymerase chain reaction (RT-PCR) and examined tissue distribution of its mRNA by RT-PCR analysis.…”

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confidence: 98%

cDNA Cloning of Feline Leptin and Its mRNA Expression in Adipose Tissue.

Sasaki

Shibata

Honjoh

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Leptin, the product of the obese (ob) gene, is an adipocyte-derived hormone involved in regulating food intake and energy expenditure in humans and rodents. To determine the primary structure of feline leptin, we cloned the feline leptin cDNA using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of complementary DNA (cDNA) ends (RACE) methods. The full-length feline leptin cDNA was 2935 bp with a 501 bp open reading frame encoding the precursor peptide of 167 amino acids including 21 residues of signal peptide. The sequence of a 146-amino acid mature leptin was 81.5-91.8% homologous to those of other species. RT-PCR analysis revealed that the leptin mRNA was expressed in adipose tissues and not detected in liver, heart, kidney, lung, pancreas, brain and skeletal muscle. These data show that feline leptin is highly homologous to leptins of other species, and expressed in adipose tissues in cats. KEY WORDS: adipose tissue, cDNA cloning, feline, leptin.

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“…In contrast, the plasma PAI-1 level was positively correlated with visceral fat area. These data suggested that the secreted PAI-1 from accumulated visceral fat may Contribute to the determination of plasma PAI-1 levels and thus, may have an important role in the development of thrombotic disorders frequently found in visceral obesity and syndrome X. Location-specificcontrol of the genes in adipose tissues Next, weinvestigated the durational changes of the leptin mRNA levels; this is another cytokine secreted from adipose tissue in VMHrats (12). Leptin mRNAabundance increased very rapidly in the visceral fat and reached a steady state level.…”

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confidence: 99%