Enhanced extraction of 3‐demethylated colchicine from fermentation broth of <i>Bacillus megaterium</i>: Optimization of process parameters by statistical experimental design

Dubey, Kashyap Kumar; Jawed, Arshad; Haque, Shafiul

doi:10.1002/elsc.201000207

Cited by 21 publications

(21 citation statements)

References 26 publications

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“…Statistical optimizations such as RSM are studies where participating parameters are varied simultaneously for optimization experiments (Greasham and Inamine, 1986; Greasham and Herber, 1997; Zhang et al, 2000; Margarita et al, 2005). RSM can collectively optimize all the affecting parameters to eliminate the limitations with single-factor optimization process (Gough et al, 1996; Dubey et al, 2011; Kanmani et al, 2013). We therefore, attempted to decipher and model glucose and NaCl content of the growth medium, using R. toruloides.…”

Section: Introductionmentioning

confidence: 99%

Concomitant Production of Lipids and Carotenoids in Rhodosporidium toruloides under Osmotic Stress Using Response Surface Methodology

et al. 2016

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As a replacement to existing fossil fuels, biofuels, have proven their worth; however, their widespread use is limited due to inconsistent yields, higher costs and poor productivity. An oleaginous yeast, Rhodosporidium toruloides has been reported to accumulate substantial amounts of lipids (that can be converted to biofuels) and therefore, it was selected for study and optimization. Apart from lipids, R. toruloides is also reported to produce carotene that can be used as a therapeutic agent. In this study, the culture medium was statistically modeled and optimized for concomitant production of lipids and carotenoids and for improving and maximizing the productivity of lipids as well as carotenes. The two metabolites were expressed differentially in the growth cycle of the organism. Culture medium components were simultaneously varied at five different levels using statistical modeling employing response surface methodology (RSM). Osmotic stress was introduced in order to simulate saline conditions and optimize the carotenoid as well as lipid production process, to be used in conditions with high salt contents. We observed a 10% (w/v) increase in carotenoid production in initial experiments under osmotic stress due to high salt concentration, while the increase in lipid synthesis was not pronounced. In this study, we demonstrate 36.2% (w/v) lipid production and 27.2% (w/v) carotenoid production, under osmotic stress with high salt concentrations, for the first time.

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Section: Introductionmentioning

confidence: 99%

Concomitant Production of Lipids and Carotenoids in Rhodosporidium toruloides under Osmotic Stress Using Response Surface Methodology

et al. 2016

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“…In such a practice interaction effect between the variables is not taken into consideration. Nowadays, RSM is a good statistical experimental design tool that has proved its worth and it is commonly used in optimisation experiments of similar processes (Alam, Muyibi, and Toramae 2007;Dubey, Jawed, and Haque 2011). RSM technique has been employed (Shieh, Liao, and Lee 2003;Yuan et al 2008) to provide a fast and efficient way to understand and identify the significant process variables, thereby saving time and maintaining key information of each component.…”

Section: Introductionmentioning

confidence: 99%

Enhancement in Jatropha-based biodiesel yield by process optimisation using design of experiment approach

Dhingra

Dubey

Bhushan

2013

International Journal of Sustainable Energy

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Edible and non-edible oils are used for the production of biodiesel from the last so many years and these oils are extracted from their respective seeds. Jatropha oil is used as a feedstock to produce biodiesel for running the Compression Ignition engine. A statistical model is developed to interrelate the transesterification process variables for the biodiesel yield using design of experiment approach by selecting central composite design of a response surface methodology. Results shown in this paper indicate that the optimum observed yield of 95.5% has the following reaction conditions: Molar ratio 19.84 (% v/v), reaction time 3 h, reaction temperature 70 • C, catalyst concentration 4.18 wt% and stirrer rpm 650. Also, the yield produced is higher when compared with 93.5% which was observed by Lee paper using the same methodology. Moreover, the fuel properties of Jatropha biodiesel are closer to the ASTM standard of biodiesel.

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“…In order to meet the market demand, several high yielding techniques have been discovered in the past, and successfully implemented in various processes, like production of primary or secondary metabolites, biotransformation, oil extraction etc. (Dubey et al, 2008, 2011; Singh et al, 2009; Rajeswari et al, 2014). …”

Section: Introductionmentioning

confidence: 99%

Strategies for Fermentation Medium Optimization: An In-Depth Review

et al. 2017

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Optimization of production medium is required to maximize the metabolite yield. This can be achieved by using a wide range of techniques from classical “one-factor-at-a-time” to modern statistical and mathematical techniques, viz. artificial neural network (ANN), genetic algorithm (GA) etc. Every technique comes with its own advantages and disadvantages, and despite drawbacks some techniques are applied to obtain best results. Use of various optimization techniques in combination also provides the desirable results. In this article an attempt has been made to review the currently used media optimization techniques applied during fermentation process of metabolite production. Comparative analysis of the merits and demerits of various conventional as well as modern optimization techniques have been done and logical selection basis for the designing of fermentation medium has been given in the present review. Overall, this review will provide the rationale for the selection of suitable optimization technique for media designing employed during the fermentation process of metabolite production.

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Enhanced extraction of 3‐demethylated colchicine from fermentation broth of Bacillus megaterium: Optimization of process parameters by statistical experimental design

Cited by 21 publications

References 26 publications

Concomitant Production of Lipids and Carotenoids in Rhodosporidium toruloides under Osmotic Stress Using Response Surface Methodology

Concomitant Production of Lipids and Carotenoids in Rhodosporidium toruloides under Osmotic Stress Using Response Surface Methodology

Enhancement in Jatropha-based biodiesel yield by process optimisation using design of experiment approach

Strategies for Fermentation Medium Optimization: An In-Depth Review

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