In this paper, the experimental study was carried to investigate the effect of angle of the anti-vortex holes on the film cooling effectiveness on a flat plate. The pressure sensitive paint technique was applied to measure the film cooling effectiveness. Two anti-vortex hole angles of 0° and 15° with respect to the primary hole were considered, and the simple cylindrical hole case was also tested. The blowing ratio based on the cylindrical hole was 0.5 and the same flow rate was kept for all anti-vortex hole cases. Results showed that the film cooling effectiveness for the anti-vortex hole cases were much higher than that of the cylindrical case. Among the anti-vortex hole cases, 15° angle anti-vortex hole case showed higher film cooling effectiveness than that by the 0° angle anti-vortex hole case.