Enhanced Fluorescence in a Scattering Medium

Abstract

Often only small amounts of sample are available for spectroscopic analytical determinations. This work investigates the enhancement of signal in columns packed with silica particles. We propose that silica particles cause the light to scatter through the column, effectively increasing optical path length. Packed columns are shown to be effective with fluorescence spectroscopy, but results were inconclusive with absorbance spectroscopy.

Measurement of Blood Dilution during Lancet-Free Blood Sampling

“…The concentration of ICG in a mixture with blood can then be determined using a fluorimeter [3]. While the fluorescent signal in a fluorimeter is predominantly determined by the concentration of the fluorophore, the signal can also be significantly influenced by light scattering from erythrocytes (red blood cells) within the solution [4]. This phenomenon suggests that the quality of a fluorescent measurement of blood dilution could be improved by incorporating a measurement of hematocrit to compensate for the concomitant scattering from erythrocytes.…”