Enhanced fluorescence quenching rate of Coumarin 102 in stimulated emission depletion

Xanthene, cyanine, oxazine and BODIPY: the four pillars of the fluorophore empire for super-resolution bioimaging

“…247 However, recent studies revealed that the fluorescence quenching rate of common coumarin dyes (coumarin 102) can be increased substantially upon raising the STED laser power and on increasing the dye concentration or excitation power, which can adversely impact the utilization of such dyes in STED nanoscopy. 248…”

“…247 However, recent studies revealed that the fluorescence quenching rate of common coumarin dyes (coumarin 102) can be increased substantially upon raising the STED laser power and on increasing the dye concentration or excitation power, which can adversely impact the utilization of such dyes in STED nanoscopy. 248 Few, pyrene-based fluorophores have also been explored in STED nanoscopy. For instance, Connor et al designed and synthesized a pyrene-ceramide conjugate PyLa-C17Cer, that not only demonstrated excellent LD-staining aptitude in living cells but also revealed outstanding STED compatibility (Fig.…”