Enhanced Gene Expression in Bacillus Subtilis by Escherichia Coli Insertion Sequences, Is5 and Is1

Takano, Teruo; Kawata, Mutsumi

doi:10.1016/b978-0-12-274155-5.50042-8

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1991

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“…Deletions in this region led to reduced levels of P-galactosidase activity, and the effect of these deletions was correlated with their extent, contrary to what might be expected for the deletion of a promoter. Alternatively, the effect of this region could be to stabilize messenger RNA transcripts initiated upstream and extending through sac Y (Tanaka & Kawata, 1986), or to enhance the activity of a promoter for sacY located at the very end of Tn917 or even downstream, in B. subtilis sequences (Reynolds et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

Dual effect of a Tn917 insertion into the Bacillus subtilis sacX gene

Coqd¹,

Aymerich²,

Steinmetz³

1991

Microbiology

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~~~~~~~~~~~ ~~The most common effect of transposon insertion is the inactivation of genes. However, in some cases, transposons can activate in cis the expression of genes in the neighbourhood of their integration site. We previously described an insertion of the transposon Tn917 into the Bacillus subtilis sacXY locus. smX and sacY encode respectively a negative and a positive regulator involved in induction by sucrose of the exoenzyme levansucrase. Data in this paper show that the Tn917 insertion had two effects: it inactivated sacXand it increased the transcription of sacX The latter effect involved one or several elements internal to the transposon.

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Section: Discussionmentioning

confidence: 99%