Enhanced glucotropaeolin production in hairy root cultures of Tropaeolum majus L. by combining elicitation and precursor feeding

Wielanek, Marzena; Urbanek, H.

doi:10.1007/s11240-006-9106-2

Cited by 55 publications

(27 citation statements)

References 45 publications

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“…Salicylic acid is an important plant signaling compound associated with stimulation of pathogen induced plant defense response and accumulation of pathogenesis related compounds [15,[38][39][40]. Stimulatory effect of salicylic acid was commonly observed on overproduction of secondary metabolites in plant cell cultures [41,42].…”

Section: Discussionmentioning

confidence: 99%

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Shinde

Malpathak

Fulzele

2009

Biotechnol Bioproc E

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^Äëíê~Åí= The effect of biotic elicitors (yeast extract, chitosan), signaling molecule (salicylic acid), and polyamines (putrescine and spermidine) was studied with respect to isoflavones accumulation in hairy root cultures of mëçê~äÉ~=ÅçêóäáÑçäá~=L. Untreated hairy roots (control) accumulated 1.55% dry wt of daidzein and 0.19% dry wt of genistein. In precursor feeding experiment, phenylalanine at 2 mM concentration led to 1.3 fold higher production of daidzein (1.91% dry wt) and genistein (0.27% dry wt). In biotic elicitors, chitosan (2 mg/L) was found to be the most efficient elicitor to induce daidzein (2.78% dry wt) and genistein (0.279% dry wt) levels in hairy roots. Salicylic acid at 1 mM concentration stimulated the maximum accumulation of daidzein (2.2% dry wt) and genistein (0.228% dry wt) 2 days after elicitation. In case of polyamines, putrescine (50 mM) resulted in highest accumulation of daidzein (3.01% dry wt) and genistein (0.227% dry wt) after 5 days of addition. Present results indicated the effectiveness of elicitation and precursor feeding on isoflavones accumulation in hairy roots of mK=ÅçêóäáÑçäá~. This is the first report of elicitation on isoflavones production by hairy roots of mK=ÅçêóäáÑçäá~. © KSBB hÉóïçêÇëW=Psoralea corylifoliaI=éÜóíçÉëíêçÖÉåëI=ÉäáÅáí~íáçåI=éêÉÅìêëçêI=ëáÖå~äáåÖ=ãçäÉÅìäÉ

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Section: Discussionmentioning

confidence: 99%

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Shinde

Malpathak

Fulzele

2009

Biotechnol Bioproc E

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“…Glucosinolates were extracted, purified and analyzed according to Wielanek and Urbanek (2006). The identification of intact glucotropaeolin, gluconasturtiin and glucoiberverin was performed by HPLC.…”

Section: Methodsmentioning

confidence: 99%

“…Myrosinase was extracted and analyzed according to the method of Wielanek and Urbanek (2006). Myrosinase activity was expressed in lmol min -1 (U) mg -1 protein determined by Bradford's method.…”

Section: Methodsmentioning

confidence: 99%

“…We have already established hairy root cultures of Tropaeolum majus that are able to produce glucotropaeolin, myrosinase and benzyl isothiocyanate. We have also reported a method to stimulate glucotropaeolin production by precursor amino acids (Phe, Cys) and elicitors (Wielanek and Urbanek 2006). The aim of this present work was to obtain hairy root cultures of Nasturtium officinale, Barbarea verna and Arabis caucasica with active glucosinolate-myrosinase system, and to study the influence of individual or combined treatment with amino acid precursors of glucosinolates (Phe, Met, Cys) and cysteine (Ser, AOS) biosynthesis on glucosinolate and isothiocyanate production.…”

Section: Introductionmentioning

confidence: 99%

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Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production

et al. 2009

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Hairy roots of Nasturtium officinale, Barbarea verna and Arabis caucasica with active glucosinolate-myrosinase system were obtained after transformation with Agrobacterium rhizogenes. Hairy roots of N. officinale produced phenylalanine-derived gluconasturtiin and glucotropaeolin (max. 24 and 7 mg g(-1) DW). B. verna and A. caucasica hairy roots produced gluconasturtiin (max. 41 mg g(-1) DW) and methionine-derived glucoiberverin (max. 32 mg g(-1) DW), respectively. Treatment of the roots with amino acid precursors of glucosinolate or/and cysteine biosynthesis increased levels of glucosinolate production, combinations of phenylalanine with cysteine (for gluconasturtiin and glucotropaeolin) and methionine with o-acetylserine (for glucoiberverin) were the most effective.

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“…Salicylic acid is an important plant signaling compound associated with stimulation of pathogen induced plant defense response and accumulation of pathogenesis related compounds [15,[34][35][36]. As a result, salicylic acid was studied for overproduction of secondary metabolites in plant cell cultures [37,38].…”

Section: Effect Of Polyamines On Cell Growth and Isoflavone Productionmentioning

confidence: 99%

Optimized production of isoflavones in cell cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Shinde

Malpathak

Fulzele

2009

Biotechnol Bioproc E

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The effect of biotic elicitors (yeast extract, chitosan), signaling molecule (salicylic acid), and polyamines (putrescine and spermidine) was studied with respect to isoflavones accumulation in cell suspension cultures of mëçê~äÉ~=ÅçêóäáÑçäá~ L. Untreated cell suspension (control) accumulated 1.66% dry wt of daidzein and 0.165% dry wt of genistein. In precursor feeding experiment, phenylalanine at 0.5 mM concentration led to 1.3 fold higher production of daidzein (1.99% dry wt) and genistein (0.22% dry wt). In biotic elicitors, yeast extract (100 mg/L) was found to be the most efficient elicitor to induce higher production levels of daidzein (2.21% dry wt) and genistein (0.293% dry wt) in suspension cultures. Salicylic acid (signaling molecule) at 1 mM concentration stimulated the maximum accumulation of daidzein (3.4% dry wt) and genistein (0.41% dry wt) 2 days after elicitation. In case of polyamines, spermidine (100 mM) resulted in highest accumulation of daidzein (3.2% dry wt) and genistein (0.475% dry wt) after 7 days of addition, which was 2.4 fold of that in control. This is the first report on kinetics of isoflavone production in response to elicitation in cell suspension of mK=ÅçêóäáÑçäá~. © KSBB hÉóïçêÇëW=Psoralea corylifolia, áëçÑä~îçåÉëI=ÉäáÅáí~íáçåI=éêÉÅìêëçêI=ëáÖå~äáåÖ=ãçäÉÅìäÉ=

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Enhanced glucotropaeolin production in hairy root cultures of Tropaeolum majus L. by combining elicitation and precursor feeding

Abstract: We have studied the effects of precursor amino acids (phenylalanine and cystein)

Cited by 55 publications

References 45 publications

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Enhanced production of phytoestrogenic isoflavones from hairy root cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production

Optimized production of isoflavones in cell cultures of Psoralea corylifolia L. Using elicitation and precursor feeding

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