Enhanced glutathione production by bifunctional enzyme coupling with ydaO-based ATP regulating system in Escherichia coli

Chen, Yawei; Cao, Yiting; Kong, Weizhen; Shi, Yiying; Cheng, Weiming

doi:10.1016/j.jff.2020.104211

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…Methylation proceess was further upregulated by regulating ATP and NADPH contents, which are important cofactors for SAM biosynthesis. Previous studies proved that ydaO from B. subtilis can dynamically regulate ATP content in E. coli [ 47 , 48 ]. The accumulation of (2 S )-sakuranetin increased by 162.38% in the ydaO -expressing strain compared with strain 7-FOMT-3.…”

Section: Discussionmentioning

confidence: 99%

Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance

Sun

Gao

et al. 2022

Synthetic and Systems Biotechnology

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Section: Discussionmentioning

confidence: 99%

Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance

Sun

Gao

et al. 2022

Synthetic and Systems Biotechnology

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“…17,34,54 To increase ATP abundance, conventional approaches primarily utilized polyphosphate and polyphosphate kinases (PPK) for ATP regeneration. 55,56 For example, the ppk from Thermus thermophiles and Sulfurovum lithotrophicum were selected for ATP regeneration to provide energy supply for cytidine triphosphate and UTP synthesis, respectively. 8,57 However, two weaknesses emerged: (1) the accumulation of free phosphate ions during polyphosphate utilization, and (2) the relatively low utilization rate of phosphate residue in polyphosphate.…”

Section: Discussionmentioning

confidence: 99%

“…An ATP-sensing switch was employed to regulate ETC to ensure a stable intracellular ATP supply and drive increased UTP titer and cell density. Previous studies have established the inextricable connection between pyrimidine derivatives accumulation and ATP levels. ,, To increase ATP abundance, conventional approaches primarily utilized polyphosphate and polyphosphate kinases (PPK) for ATP regeneration. , For example, the ppk from Thermus thermophiles and Sulfurovum lithotrophicum were selected for ATP regeneration to provide energy supply for cytidine triphosphate and UTP synthesis, respectively. , However, two weaknesses emerged: (1) the accumulation of free phosphate ions during polyphosphate utilization, and (2) the relatively low utilization rate of phosphate residue in polyphosphate . To address these limitations, we focused on ETC, which tightly couples oxidative phosphorylation with ATP synthase to facilitate ATP generation.…”

Section: Discussionmentioning

confidence: 99%

Multivariate Modular Metabolic Engineering for High Titer Uridine Triphosphate Production in Escherichia coli

Liu,

Chen,

et al. 2023

ACS Sustainable Chem. Eng.

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Uridine triphosphate (UTP) holds great potential for applications in the food and medicine fields. Compared with the chemical synthesis route, microbial biosynthesis of UTP through metabolic engineering approaches offers a sustainable and environmentally friendly alternative. However, the tightly regulated UTP metabolic pathway has presented challenges in building efficient microbial cell factories. To address this issue, we applied a modular metabolic engineering strategy to overcome four distinct bottlenecks in UTP production in Escherichia coli. First, by blocking the UTP catabolic module, we achieved a substantial accumulation of UTP up to 304.67 μM. Second, in the UTP synthetic module, a uridine 5′monophosphate (UMP) kinase variant (MtUMPK R141A/K148A ) was obtained based on crystal structure analysis, resulting in a 10.47-fold increase in catalytic capacity. Furthermore, by fusion expression of the UMP kinase variant and orotidine 5′phosphate decarboxylase, the conversion of UMP to UTP was improved, leading to UTP concentrations reaching 11.36 mM. Third, in the UTP precursor module, several key metabolism-related genes were engineered to enhance the supply of precursors, resulting in an 87.9% increase in UTP production. Finally, by implementing electron transport chain regulation with an ATP-sensing switch, the optimal strain E. coli K16 achieved an intracellular UTP concentration of 28.40 mM (13.75 g/L), which was 544.37-fold higher than that of the base strain. This study represents the design of a de novo UTP-producing strain through metabolic engineering, opening new avenues for UTP and its derivatives production.

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De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid

Ge,

Wang,

et al. 2025

Nat Commun

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Enhanced glutathione production by bifunctional enzyme coupling with ydaO-based ATP regulating system in Escherichia coli

Cited by 3 publications

References 27 publications

Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance

Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance

Multivariate Modular Metabolic Engineering for High Titer Uridine Triphosphate Production in Escherichia coli

De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid

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