Enhanced growth of tumour cells in healing colonie anastomoses and laparotomy wounds

Abstract

In the past, it has been noted that experimental tumour cells inoculated into the peritoneal cavity or into the lumen of the bowel will grow at a recently formed colonic anastomosis. However, it has previously been unclear whether the healing process enhances tumour growth or whether the presence of a suture line merely allows the tumour cells to gain access to the tissues. In the present study, using the hooded Lister rat, we have confirmed these findings by showing that growth of the syngeneic MC28 sarcoma and OES5 breast carcinoma occurs preferentially at colonic anastomoses and laparotomy wounds after intraperitoneal injection, and at colonic anastomoses after intraluminal injection. In previous studies using the MC28 sarcoma and the OES5 breast carcinoma injected by the intracardiac route (so that tumour cells reach normal and healing tissues in approximately equal numbers) we have shown that tumour growth is enhanced in healing wounds but not in the surrounding normal tissues when cells reach a healing colonic anastomosis or laparotomy wound within 2 h of its formation. Furthermore, by studying the distribution of radiolabelled tumour cells after intracardiac injection, we have calculated that the probability of a tumour cell leading to a deposit in a healing anastomosis or laparotomy wound is increased 1,000 fold compared to normal tissue. No previous studies have combined the data for intracardiac, intraluminal and intraperitoneal injection of tumour cells using the same animal model. We conclude that the same phenomenon of tumour growth enhancement in colonic anastomoses and laparotomy wounds reported after intracardiac injection of tumour cells may well be enhancing tumour growth after intraperitoneal and intraluminal injection.(ABSTRACT TRUNCATED AT 250 WORDS)

The role of endotoxin/lipopolysaccharide in surgically induced tumour growth in a murine model of metastatic disease

“…Therefore, the increased metastatic growth observed in animals exposed to air during the surgical procedure cannot be attributed to the removal of antiangiogenic factors. The tumour enhancing effect of open surgery has been demonstrated previously (Skipper et al, 1989;Arai et al, 1992;Kodama et al, 1992), but the mechanisms by which surgery increases tumour growth have remained unclear. Both laparotomy and air contamination of the peritoneum have previously been shown to result in bacterial translocation across the gut (Watson et al, 1995), and we observed high plasma levels of LPS in animals 4 h after i.p injection of LPS.…”

A Case of Parastomal Hernia with Isolated Abdominal Wall Metastasis of Colorectal Cancer

“…Cutaneous metastasis can be seen in the old operation scars due to changes in the microscopic anatomy of the lymphatic channels, the changes in adhesion molecular profile or local immune response around the scar. These are eligible sites for metastasis of the tumor cells (13). Evidence from clinical and experimental studies suggests that surgical trauma may promote tumor metastasis to the abdominal wall (14).…”

“…The importance of exfoliated cancer cells has been studied by several authors (15). A mechanism more likely responsible for abdominal wall metastasis is direct implantation of liberated cancer cells (13). It is thought that cancer cells may be present in the peritoneal cavity before the surgical procedure or may be exfoliated during operative manipulation (12).…”

Trokarkanalmetastasen: Was ist gesichert?

“…Die Ergebnisse dieser experimentellen Studien sind allerdings kontrovers und keinesfalls eindeutig. Jacobi et al Lokale Wundbedingungen an den Trokarinzisionen Seit langem ist aus der konventionellen Chirurgie bekannt, dass lokale Heilungsprozesse Adha È renz und Wachstum von Tumorzellen beeinflussen und fo È rdern [49]. So fu È hrte die intrakardiale, intraperitoneale oder die intravaskula Ère Injektion von Tumorzellen an einer Darmanastomose oder in Wundbereiche in einem Rattenmodell zu einer tausendfach ho È heren Tumorabsiedlung als in anderen Geweben [49].…”