Enhanced gymnemic acids production in cell suspension cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm. through elicitation

Mahendran, G.; Kumar, Deepak; Verma, Sanjeet K.; Rout, Prasant Kumar; Rahman, Laiq ur

doi:10.1016/j.indcrop.2020.113234

Cited by 24 publications

(4 citation statements)

References 74 publications

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“…The CHI stock solution was prepared at 100 mg/mL and used at concentrations of 25, 50, 75, and 100 mg/L. Then, the culture was shaken at 150 rpm in the darkness for another 3 or 6 days ( Mahendran et al., 2021 ). In addition, the elicitors’ solvents were tested on the root culture to ensure non-interference of the solvents on the elicitation.…”

Section: Methodsmentioning

confidence: 99%

“…Currently, hairy root culture is a potential alternative method for the production of various classes of secondary metabolites. Hairy roots usually grow faster and display greater potential to produce valuable metabolites than wild-type roots ( Giri and Narasu, 2000 ), and they can also be elicited with both biotic and abiotic agents to increase the production of bioactive metabolites ( Park et al., 2020 ; Mahendran et al., 2021 ). The main biotic elicitors used for secondary metabolite production are methyl jasmonate (MeJA), jasmonic acid, acetylsalicylic acid, chitosan (CHI), coronatine, pectin, salicylic acid (SA), and yeast extract (YE) ( Alcalde et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Elicitation enhances the production of friedelin and epifriedelanol in hairy root cultures of Cannabis sativa L.

Kobtrakul,

Rani,

Binalee

et al. 2023

Front. Plant Sci.

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Cannabis sativa L. (hemp) has a global distribution and social impact, and it is widely used as a medicinal plant, food ingredient, and textile fiber. Its roots have received less attention than other parts, especially the inflorescence, leaves, and shoots. Triterpenoids, including friedelin and epifriedelanol, have been found in hemp roots, and their anti-inflammatory effects have been reported. In this study, the potential enhancement of triterpenoid accumulation in the roots of C. sativa by elicitation was examined. Hairy roots were successfully established, and they contained 2.02-fold higher triterpenoid levels than natural roots. Furthermore, hairy roots treated with 75 μM salicylic acid had 1.95-fold higher friedelin levels (0.963 mg/g DW) and 1.4-fold higher epifriedelanol levels (0.685 mg/g DW) than untreated hairy roots. These results suggested that the elucidation of hairy root cultures using an optimized elicitor could represent an alternative strategy to produce the valuable triterpenoids friedelin and epifriedelanol.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Elicitation enhances the production of friedelin and epifriedelanol in hairy root cultures of Cannabis sativa L.

Kobtrakul,

Rani,

Binalee

et al. 2023

Front. Plant Sci.

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“…Examples of secondary metabolites obtained using suspension cultures can be given as stigmasterol from Abutilon indicum ( Rao et al., 2021 ); gymnemic acids from Gymnema sylvestre ( Mahendran et al., 2021 ); catechin from Camellia sinensis ( Ardianto et al., 2020 ); alkaloids (vincristine, vinblastine, ajmalicine and serpentine) from Catharanthus roseus ( Mishra et al., 2019 ); triterpenoids from Ocimum basilicum ( Pandey et al., 2019 ); artemisinin from Artemisia annua ( Mir et al., 2017 ); plumbagin from Plumbago europaea ( Beigmohamadi et al., 2019 ) and P. zeylanica ( Roy and Bharadvaja, 2019 ); bacoside from Bacopa monnieri ( Kharde et al., 2018 ); hydrolyzable tannin from Phyllanthus debilis ( Malayaman et al., 2017 ); triterpenoids from Euphorbia hirta ( Samkumar et al., 2019 ); atropine from Hyoscyamus muticus ( Abdelazeez et al., 2022 ); triterpenic acids (betulinic acid, oleanolic acid, and ursolic acid) from Thymus persicus (Lamiaceae) ( Bakhtiar and Mirjalili, 2022 ); and withanolides (withaferin A and withanolide A) from Withania coagulans ( Mirjalili and Esmaeili, 2022 ).…”

Section: Tissue Culture-based Biotechnological Approaches For Obtaini...mentioning

confidence: 99%

Production of secondary metabolites using tissue culture-based biotechnological applications

et al. 2023

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Plants are the sources of many bioactive secondary metabolites which are present in plant organs including leaves, stems, roots, and flowers. Although they provide advantages to the plants in many cases, they are not necessary for metabolisms related to growth, development, and reproduction. They are specific to plant species and are precursor substances, which can be modified for generations of various compounds in different plant species. Secondary metabolites are used in many industries, including dye, food processing and cosmetic industries, and in agricultural control as well as being used as pharmaceutical raw materials by humans. For this reason, the demand is high; therefore, they are needed to be obtained in large volumes and the large productions can be achieved using biotechnological methods in addition to production, being done with classical methods. For this, plant biotechnology can be put in action through using different methods. The most important of these methods include tissue culture and gene transfer. The genetically modified plants are agriculturally more productive and are commercially more effective and are valuable tools for industrial and medical purposes as well as being the sources of many secondary metabolites of therapeutic importance. With plant tissue culture applications, which are also the first step in obtaining transgenic plants with having desirable characteristics, it is possible to produce specific secondary metabolites in large-scale through using whole plants or using specific tissues of these plants in laboratory conditions. Currently, many studies are going on this subject, and some of them receiving attention are found to be taken place in plant biotechnology and having promising applications. In this work, particularly benefits of secondary metabolites, and their productions through tissue culture-based biotechnological applications are discussed using literature with presence of current studies.

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“…For example, Chavan et al [160] reported that the application of jasmonic acid (75 µM) in callus cultures in Salacia chinensis improved the total phenolic, flavonoid, and mangiferin contents for the same application, which revealed the highest antioxidant potential. Moreover, Mahendran et al [161] documented that Gymnemasylvestre cell suspension culture with 20 µM sodium nitroprusside treatments revealed the highest accumulation of deacylgymnic acid and XVII gymnemic acid. Furthermore, the cultivation of Carum copticum under salt stress enhanced the phenolic content accumulation and antioxidant activity [162].…”

Section: Elicitation Of In Vitro Productsmentioning

confidence: 99%

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

et al. 2022

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Medicinal plants are rich sources of bioactive compounds widely used as medicaments, food additives, perfumes, and agrochemicals. These secondary compounds are produced under stress conditions to carry out physiological tasks in plants. Secondary metabolites have a complex chemical structure with pharmacological properties. The widespread use of these metabolites in a lot of industrial sectors has raised the need to increase the production of secondary metabolites. Biotechnological methods of cell culture allow the conservation of plants, as well as the improvement of metabolite biosynthesis and the possibility to modify the synthesis pathways. The objective of this review is to outline the applications of different in vitro culture systems with previously reported relevant examples for the optimal production of plant-derived secondary metabolites.

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Enhanced gymnemic acids production in cell suspension cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm. through elicitation

Cited by 24 publications

References 74 publications

Elicitation enhances the production of friedelin and epifriedelanol in hairy root cultures of Cannabis sativa L.

Elicitation enhances the production of friedelin and epifriedelanol in hairy root cultures of Cannabis sativa L.

Production of secondary metabolites using tissue culture-based biotechnological applications

Biotechnology and In Vitro Culture as an Alternative System for Secondary Metabolite Production

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