Enhanced human bone marrow mesenchymal stromal cell adhesion on scaffolds promotes cell survival and bone formation

Mebarki, Miryam; Coquelin, Laura; Layrolle, Pierre; Battaglia, Séverine; Marine, Tossou; Hernigou, Philippe; Rouard, Hélène; Chevallier, Nathalie

doi:10.1016/j.actbio.2017.06.018

Cited by 73 publications

(58 citation statements)

References 53 publications

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“…This density does not seem to be a limiting factor. Indeed, the best results in several similar studies were obtained on granules with densities ranging from 90,000 to 125,000 cells per milligram (Léotot et al, ; Mankani, Kuznetsov, Fowler, Kingman, & Robey, ; Mebarki et al, ). It should be noted, however, that the presence or absence of vessels was not assessed in these studies.…”

Section: Discussionmentioning

confidence: 97%

“…We did not observe a direct contribution of transplanted WJMSC‐OBs to bone formation; no human‐osteopontin‐positive cells were detected in the implant. To the best of our knowledge, the studies that found a direct contribution of implanted cells in bone regeneration were always performed with MSCs and not OBs derived from MSCs (Mebarki et al, ). Differentiated cells might not be able to integrate into the neotissues.…”

Section: Discussionmentioning

confidence: 99%

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Co‐transplantation of Wharton's jelly mesenchymal stem cell‐derived osteoblasts with differentiated endothelial cells does not stimulate blood vessel and osteoid formation in nude mice models

Naudot

Barre

Caula

et al. 2020

J Tissue Eng Regen Med

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A major challenge in bone tissue engineering is the lack of post‐implantation vascular growth into biomaterials. In the skeletal system, blood vessel growth appears to be coupled to osteogenesis—suggesting the existence of molecular crosstalk between endothelial cells (ECs) and osteoblastic cells. The present study (performed in two murine ectopic models) was designed to determine whether co‐transplantation of human Wharton's jelly mesenchymal stem cell‐derived osteoblasts (WJMSC‐OBs) and human differentiated ECs enhances bone regeneration and stimulates angiogenesis, relative to the seeding of WJMSC‐OBs alone. Human WJMSC‐OBs and human ECs were loaded into a silicate‐substituted calcium phosphate (SiCaP) scaffold and then ectopically implanted at subcutaneous or intramuscular sites in nude mice. At both subcutaneous and intramuscular implantation sites, we observed ectopic bone formation and osteoids composed of host cells when WJMSC‐OBs were seeded into the scaffold. However, the addition of ECs was associated with a lower level of osteogenesis, and we did not observe stimulation of blood vessel ingrowth. in vitro studies demonstrated that WJMSC‐OBs lost their ability to secrete vascular endothelial growth factor and stromal cell‐derived factor 1—including when ECs were present. In these two murine ectopic models, our cell‐matrix environment combination did not seem to be optimal for inducing vascularized bone reconstruction.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Co‐transplantation of Wharton's jelly mesenchymal stem cell‐derived osteoblasts with differentiated endothelial cells does not stimulate blood vessel and osteoid formation in nude mice models

Naudot

Barre

Caula

et al. 2020

J Tissue Eng Regen Med

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“…Importantly, enhanced MSC adhesion was recently demonstrated to promote cell survival and bone formation (Mebarki et al, ). Because the 152RM peptide greatly enhanced MSCs adhesion and increased the ratio and quantity of MSCs retained in the SCR procedure, future in vivo studies might demonstrate an enhanced bone formation capability of the 152RM peptide group.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of protein‐tyrosine phosphatase 1B phosphorylation enhances early adhesion of mesenchymal stem cells to facilitate fabrication of tissue‐engineered bone

Luo

Tang

Gao

et al. 2020

J Tissue Eng Regen Med

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Enhancement of cell-matrix adhesion is preferable and crucial in various fields of tissue engineering. Integrins are important receptors that facilitate cell-matrix adhesion, mediated by intracellular molecules and crosstalk with the cadherin adhesion pathway, which mainly facilitates cell-cell adhesion. Protein-tyrosine phosphatase 1B (PTP1B) has emerged as a pivot in the crosstalk between the cadherin adhesion pathway and the integrin adhesion pathway. The phosphorylation state of PTP1B tyrosine-152 (Y152) plays a central role in balancing the two different cell adhesion forms. In this study, a PTP1B Y152 region mimicking (152RM) peptide was designed to decrease the phosphorylation of PTP1B Y152 via competitive inhibition. As a result, the dissociation of cadherin complexes and the release of PTP1B from cadherin had sharply increased, and Src, an important intracellular component of integrin, was activated, indicating that the cadherin adhesion pathway was inhibited, whereas the integrin adhesion pathway was enhanced. Moreover, upon treatment with the 152RM peptide, we observed that the early adhesion of human bone marrow-derived mesenchymal stem cells (MSCs) was accelerated and the anchoring of MSCs on the surface of integrin ligands was enhanced by an enhanced matrix adhesion ability of MSCs themselves. Importantly, the 152RM peptide significantly promoted the adhesion efficiency of MSCs in the selective cell retention technology, which fabricates instant bone implants in clinical settings, to stimulate osteogenesis in vivo. K E Y W O R D S cell adhesion, mesenchymal stem cell, PTP1B, selective cell retention, tissue engineering

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“…Studies on animal models shown that differences in bone formation depend on the biomaterial's capacity to select and guide MSCs behavior. In bone tissue engineering, scaffolds provide a mechanical and biochemical microenvironment for MSCs [6]. The MTT assay also enables the assessment of viability of cells in contact with studied biomaterials [16].…”

Section: Mtt Testmentioning

confidence: 99%

In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation

Tanasie¹,

Bojin²,

Tatu³

et al. 2017

Mat.Plast.

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Mesenchymal stem cells (MSCs) are increasingly being used in regenerative medicine to repair various tissue types. This study aimed to assess changes in MSCs properties as a result of their interaction with two biomaterials in order to evaluate their in vitro compatibility. We have used two types of biomaterials, as follows: hydroxyapatite anodized titanium (HA-Ti) is used in bone reconstruction; it is hard and remains in the body, while patches of collagen combined with poly-e-caprolactone (C-PCL) are utilized in the reconstruction of soft tissue (particularly the skin); these patches resorb in the body, being only a temporary matrix that fosters and accelerates tissue formation. We have chosen to assess changes in morphology, viability and proliferation of MSCs obtained by lipoaspirate, through direct contact with the biomaterial or in eluate. MSCs properties were assessed by optical microscopy, fluorescence microscopy after acridine orange/ethidium bromide (AO/EB) staining, and the MTT assay. After result interpretation, we gained an image of the cytotoxicity phenomenon seen during the experiment and were able to assess viability of cell in contact with the biomaterials used. HA-Ti shows better interaction with cells, which preserve their viability, and does not influence MSCs growth, proliferation or morphology. In contrast, cells on C-PCL matrix become small, spherical, were detached from the plate and had lower viability percentage.

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Enhanced human bone marrow mesenchymal stromal cell adhesion on scaffolds promotes cell survival and bone formation

Cited by 73 publications

References 53 publications

Co‐transplantation of Wharton's jelly mesenchymal stem cell‐derived osteoblasts with differentiated endothelial cells does not stimulate blood vessel and osteoid formation in nude mice models

Co‐transplantation of Wharton's jelly mesenchymal stem cell‐derived osteoblasts with differentiated endothelial cells does not stimulate blood vessel and osteoid formation in nude mice models

Inhibition of protein‐tyrosine phosphatase 1B phosphorylation enhances early adhesion of mesenchymal stem cells to facilitate fabrication of tissue‐engineered bone

In Vitro Effects of Biomaterials on Mesenchymal Stem Cells Viability and Proliferation

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