Enhanced human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor coupled with online recovery system

Ercan, Duygu; Demirci, Ali

doi:10.1016/j.bej.2015.02.032

Cited by 9 publications

(3 citation statements)

References 21 publications

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“…At the same time, they are called as microbial cell layers, which are attached on the support materials. Due to high biomass concentration, stability, and potential of long-term fermentation of cells attached on the surface materials, biofilm reactors can be used for production of value-added products such as human lysozyme (Ercan and Demirci 2015b), ethanol (Germec et al 2015(Germec et al , 2016, lactic acid (Ho et al 1997), kojic acid (Liu et al 2016b), isoflavone aglycones (Liu et al 2016a), etc. Nonetheless, microparticles used as a novel approach to improve morphology engineering of filamentous microorganisms and increase their productivity are also applied.…”

Section: Introductionmentioning

confidence: 99%

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

et al. 2017

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Mannanases, one of the important enzyme group for industry, are produced by numerous filamentous fungi, especially Aspergillus species with different fermentation methods. The aim of this study was to show the best fermentation method of β-mannanase production for fungal growth in fermenter. Therefore, different fermentation strategies in fed-batch fermentation (suspended, immobilized cell, biofilm and microparticle-enhanced bioreactor) were applied for β-mannanase production from glucose medium (GM) and carob extract medium (CEM) by using recombinant Aspergillus sojae. The highest β-mannanase activities were obtained from microparticle-enhanced bioreactor strategy. It was found to be 347.47 U/mL by adding 10 g/L of AlO to GM and 439.13 U/mL by adding 1 g/L of talcum into CEM. The maximum β-mannanase activities for suspended, immobilization, and biofilm reactor remained at 72.55 U/mL in GM, 148.81 U/mL in CEM, and 194.09 U/mL in GM, respectively. The reason for that is the excessive, and irregular shaped growth and bulk formation, inadequate oxygen transfer or substrate diffusion in bioreactor. Consequently, the enzyme activity was significantly enhanced by addition of microparticles compared to other fed-batch fermentation strategies. Also, repeatable β-mannanase activities were obtained by controlling of the cell morphology by adding microparticle inside the fermenter.

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Section: Introductionmentioning

confidence: 99%

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

et al. 2017

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“…More recently, Ercan and Demirci [ 110 , 111 , 112 , 113 ] performed stepped studies on the production of human lysozyme using the fungi Kluyveromyces lactis on PCS-grid biofilm reactors. Lysozyme is a lytic enzyme targeting bacterial cell walls with application in medicine, cosmetics, and food industries.…”

Section: Production Of Added-value Chemicalsmentioning

confidence: 99%

“…Continuous fermentation also supported significantly higher productivity (7.5 U·mL −1 ·h −1 ) over batch and fed-batch fermentations in a biofilm reactor and suspended cell reactor (4 U·mL −1 ·h −1 ). Finally, fermentation in a biofilm reactor was coupled to an online recovery system using silicic acid as an adsorbent to enhance lysozyme production and recovery [ 113 ]. The adsorption and desorption conditions of the recovery system were optimized, accomplishing 96% lysozyme adsorption and 98% desorption.…”

Section: Production Of Added-value Chemicalsmentioning

confidence: 99%

Advances on Bacterial and Fungal Biofilms for the Production of Added-Value Compounds

Carvalho

Azevedo

Ferreira

et al. 2022

Biology

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In recent years, abundant research has been performed on biofilms for the production of compounds with biotechnological and industrial relevance. The use of biofilm platforms has been seen as a compelling approach to producing fine and bulk chemicals such as organic acids, alcohols, and solvents. However, the production of recombinant proteins using this system is still scarce. Biofilm reactors are known to have higher biomass density, operational stability, and potential for long-term operation than suspended cell reactors. In addition, there is an increasing demand to harness industrial and agricultural wastes and biorefinery residues to improve process sustainability and reduce production costs. The synthesis of recombinant proteins and other high-value compounds is mainly achieved using suspended cultures of bacteria, yeasts, and fungi. This review discusses the use of biofilm reactors for the production of recombinant proteins and other added-value compounds using bacteria and fungi.

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Productive biofilms: from prokaryotic to eukaryotic systems

Schmeckebier

Zayed

Ulber

2022

J of Chemical Tech & Biotech

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Biofilms have great potential for producing valuable products more efficiently than suspended cultivations when it comes to yield and productivity. In addition, biofilms have the advantage of cell protection and increased resistance to environmental influences. Therefore, productive biofilms are used in biofilm reactors to produce value‐added products. A summary of possible applications for biofilm reactors is given here. In addition, the surfaces on which biofilms are cultivated are briefly explained. The possibility of using biofilms is not limited to prokaryotic systems. Thus, eukaryotic systems can also be cultivated as biofilms to produce valuable products. Therefore, both prokaryotic and eukaryotic productive biofilms will be described in this review. In the case of eukaryotic systems, fungal biofilms and plant biofilms will be discussed. The biofilm formation of the different cell types is listed and the productivity of a cell line on a corresponding substrate is summarized. Finally, the investigation methods of biofilms are discussed, as these methods are essential to understanding biofilms. © 2022 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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Enhanced human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor coupled with online recovery system

Cited by 9 publications

References 21 publications

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

Advances on Bacterial and Fungal Biofilms for the Production of Added-Value Compounds

Productive biofilms: from prokaryotic to eukaryotic systems

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