Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots

St‐Arnaud, Marc; Hamel, Chantal; Vimard, B.; Caron, M.; Fortin, J. A.

doi:10.1016/s0953-7562(96)80164-x

Cited by 397 publications

(266 citation statements)

References 17 publications

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“…After 35±45 days, fresh roots were harvested for immunolocalisation studies and the remaining tissue was frozen in liquid N 2 and stored at ±80°C for RNA and protein isolation. Glomus intraradices was cultured with carrot hairy roots in two-compartment Petri-plates (St-Arnaud et al, 1996) and spores were prepared from these plates according to Be Â card and Fortin (1988). Inoculation of plants with Glomus intraradices spores was carried out according to Harrison and Dixon (1993).…”

Section: Methodsmentioning

confidence: 99%

The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface

Chiou¹,

Liu²,

Harrison³

2001

The Plant Journal

185

145

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SummaryThe movement of phosphate from the soil into plant root cells is the ®rst of many crucial transport events required to supply phosphorous (P) to cells throughout the plant. In addition to the ability to acquire phosphate from the soil, the majority of the vascular plants are able to form arbuscular mycorrhizal associations in which phosphate may be delivered to the cortex via a fungus. Previously, we cloned two phosphate transporter genes, MtPT1 and MtPT2 from Medicago truncatula roots. Complementation of a yeast phosphate transport mutant revealed that MtPT1 is a functional phosphate transporter and Northern analyses revealed that MtPT1 is expressed exclusively in roots (Liu et al., 1998, Mol. Plant-Microbe Interact. 11, 14±22). Utilising an antibody speci®c for MtPT1, we have analysed the accumulation and spatial expression patterns of the MtPT1 transporter. MtPT1 transcript and protein levels show close correlation and increase dramatically in the roots in response to phosphate starvation. MtPT1 protein levels decrease in roots during development of a symbiosis with arbuscular mycorrhizal (AM) fungi, indicating that this transporter is not involved in symbiotic phosphate transport. Membrane fractionation and analysis of a MtPT1/GFP fusion protein revealed that MtPT1 is located in the plasma membrane, while in situ hybridisation and immunolocalisation demonstrate the presence of MtPT1 transcripts and protein in the epidermal cells and root hairs of M. truncatula roots. MtPT1 shows expression patterns consistent with a role speci®cally in the acquisition of phosphate from the soil and is distinct from the other phosphate transporter of this class described to date.

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Section: Methodsmentioning

confidence: 99%

The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface

Chiou¹,

Liu²,

Harrison³

2001

The Plant Journal

185

145

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“…Cultures of Ri T-DNA-transformed carrot (Daucus carota L.) roots colonized by Glomus intraradices (Schenck and Smith) were provided by Dr Chantal Hamel, Department of Natural Resource Sciences, McGill University, Montreal, Canada (St Arnaud et al, 1996). Mycorrhiza stock cultures for the following experiments were maintained in the dark at 27mC on a nutrient medium (Be!…”

Section: General Methodsmentioning

confidence: 99%

Extraradical hyphae of the mycorrhizal fungus Glomus intraradices can hydrolyse organic phosphate

2000

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Organic phosphorus sources make up a large fraction of the total P in some soils. Vesicular-arbuscular mycorrhizal fungi provide a large surface area for the absorption of inorganic P. The question of whether or not they have direct access to organic P by producing extracellular phosphatases has hitherto been controversial because experiments had not been performed in the absence of other soil microorganisms. We used a split-dish in vitro carrot mycorrhiza system free from contaminating microorganisms. The extraradical hyphae of Glomus intraradices hydrolysed both 5-bromo-4-chloro-3-indolyl phosphate and phenolphthalein diphosphate. Moreover, they transferred significantly more P to roots when they had access to inositol hexaphosphoric acid (phytate) than when they did not. Thus we show unequivocally that extraradical hyphae of G. intraradices can hydrolyse organic P, and, further, that the resultant inorganic P can be taken up and transported to host roots.

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“…In vitro cultures of AM fungus Rhizophagus irregularis DAOM 197198 with Agrobacterium rhizogenes (Ri T-DNA) transformed carrot (Daucus carota L.) roots in solid M medium gelled with 0.4% (w/v) phytagel was established according to St-Arnaud et al's methods [25]. Detailed methods are described in the Supplementary Information.…”

Section: Root-organ Culturesmentioning

confidence: 99%

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

Zhang

Sun

et al. 2016

Journal of Hazardous Materials

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Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots

Cited by 397 publications

References 17 publications

The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface

The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface

Extraradical hyphae of the mycorrhizal fungus Glomus intraradices can hydrolyse organic phosphate

Chromium immobilization by extra- and intraradical fungal structures of arbuscular mycorrhizal symbioses

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