Enhanced Image Sensor Module for Head-Mounted Microscopes

Juneau, Jill; Duret, Guillaume; Robinson, Jacob T.; Kemere, Caleb

doi:10.1109/embc.2018.8512387

Cited by 6 publications

(4 citation statements)

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“…Furthermore, there are limitations of the imaging sensors used in miniscopes. However, genetically encoded voltage indicators (GEVIs) ( Bando et al, 2019 ) and improved image sensors ( Marshall et al, 2016 ; Juneau et al, 2018 ) offer a new horizon for increasing the temporal precision of miniscopes.…”

Section: Discussionmentioning

confidence: 99%

Circuit Investigation of Social Interaction and Substance Use Disorder Using Miniscopes

Beacher

Washington

Werner

et al. 2021

Front. Neural Circuits

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Substance use disorder (SUD) is comorbid with devastating health issues, social withdrawal, and isolation. Successful clinical treatments for SUD have used social interventions. Neurons can encode drug cues, and drug cues can trigger relapse. It is important to study how the activity in circuits and embedded cell types that encode drug cues develop in SUD. Exploring shared neurobiology between social interaction (SI) and SUD may explain why humans with access to social treatments still experience relapse. However, circuitry remains poorly characterized due to technical challenges in studying the complicated nature of SI and SUD. To understand the neural correlates of SI and SUD, it is important to: (1) identify cell types and circuits associated with SI and SUD, (2) record and manipulate neural activity encoding drug and social rewards over time, (3) monitor unrestrained animal behavior that allows reliable drug self-administration (SA) and SI. Miniaturized fluorescence microscopes (miniscopes) are ideally suited to meet these requirements. They can be used with gradient index (GRIN) lenses to image from deep brain structures implicated in SUD. Miniscopes can be combined with genetically encoded reporters to extract cell-type specific information. In this mini-review, we explore how miniscopes can be leveraged to uncover neural components of SI and SUD and advance potential therapeutic interventions.

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Section: Discussionmentioning

confidence: 99%

Circuit Investigation of Social Interaction and Substance Use Disorder Using Miniscopes

Beacher

Washington

Werner

et al. 2021

Front. Neural Circuits

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“…One key next step will be the refinement of SNR by using better imaging sensors [13], improved transmission optics and brighter bioluminescence constructs. CMOS sensors offering higher sensitivity are widely available and, as seen in Fig.…”

Section: Discussionmentioning

confidence: 99%

Miniaturized Devices for Bioluminescence Imaging in Freely Behaving Animals

Celinskis

Friedman

Koksharov

et al. 2020

2020 42nd Annual International Conference of the IEEE Engineering in Medicine &Amp; Biology Society (EMBC)

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In vivo fluorescence miniature microscopy has recently proven a major advance, enabling cellular imaging in freely behaving animals. However, fluorescence imaging suffers from autofluorescence, phototoxicity, photobleaching and nonhomogeneous illumination artifacts. These factors limit the quality and time course of data collection. Bioluminescence provides an alternative kind of activity-dependent light indicator. Bioluminescent calcium indicators do not require light input, instead generating photons through chemiluminescence. As such, limitations inherent to the requirement for light presentation are eliminated. Further, bioluminescent indicators also do not require excitation light optics: the removal of these components should make a lighter and lower cost microscope with fewer assembly parts. While there has been significant recent progress in making brighter and faster bioluminescence indicators, the advances in imaging hardware have not yet been realized. A hardware challenge is that despite potentially higher signal-to-noise of bioluminescence, the signal strength is lower than that of fluorescence. An open question we address in this report is whether fluorescent miniature microscopes can be rendered sensitive enough to detect bioluminescence. We demonstrate this possibility in vitro and in vivo by implementing optimizations of the UCLA fluorescent miniscope v3.2. These optimizations yielded a miniscope (BLmini) which is 22% lighter in weight, has 45% fewer components, is up to 58% less expensive, offers up to 15 times stronger signal and is sensitive enough to capture spatiotemporal dynamics of bioluminescence in the brain with a signal-to-noise ratio of 34 dB.

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“…Here, we used a micro-endoscope to record neural activity in the CA1 region of dorsal hippocampus, while animal performed the SAL-VR task. Our modified Miniscope uses a customized housing and baseplate [12], which allows for ease of manipulation when headmounting the animals, in comparison to e.g. the standard UCLA Miniscope setup.…”

Section: Calcium Imaging Using 1p Miniaturized Microscopementioning

confidence: 99%