Enhanced Immunogenicity of a Genetic Chimeric Protein Consisting of Two Virulence Antigens of<i>Streptococcus mutans</i>and Protection against Infection

Zhang, Ping; Jespersgaard, Christina; Lamberty-Mallory, Leticia; Katz, Jannet; Huang, Yan; Hajishengallis, George; Michalek, Suzanne M.

doi:10.1128/iai.70.12.6779-6787.2002

Cited by 52 publications

(51 citation statements)

References 48 publications

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“…It was demonstrated that KF-rPAc could promote significant higher rPAc-specific antibodies in serum as well as in saliva compared with the rPAc alone or mixture of rPAc and KF (KF+rPAc). Previous studies usually used at least 50 μg dosage of PAc protein, chimeric protein SBR-GLU, or CTB-PAc coupled immunogen for inducing detectable mucosal immune responses or protective salivary IgA antibody against S. mutans infection in rats [62,63]. By flagellin-PAc fusion strategy, 4.2 μg of KF-rPAc, which is equivalent to 2.5 μg rPAc, could induce high levels of immune responses; and 8.5 μg KF-rPAc could induce significantly elevated antibody responses and confer effective reduction of dental caries in rats.…”

Section: Enhancement Of Salivary Siga Antibodies and Anti-caries Protmentioning

confidence: 99%

Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Yan

2013

Sci. China Life Sci.

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Dental caries remains one of the most common global chronic diseases caused by Streptococcus mutans, which is prevalent all over the world. The caries prevalence of children aged between 5-6 years old in China is still in very high rate. A potent and effective anti-caries vaccine has long been expected for caries prevention but no vaccines have been brought to market till now mainly due to the low ability to induce and maintain protective antibody in oral fluids. This review will give a brief historical retrospect on study of dental caries and pathogenesis, effective targets for anti-caries vaccines, oral immune system and immunization against dental caries. Then, salivary IgA antibodies and the protective responses are discussed in the context of the ontogeny of mucosal immunity to indigenous oral streptococcal. The methods and advancement for induction of specific anticaries salivary sIgA antibodies and enhancement of specific anti-caries salivary sIgA antibodies by intranasal immunization with a safe effective mucosal adjuvant are described. The progress in the enhancement of salivary sIgA antibodies and anticaries protection by intranasal immunization with flagellin-PAc fusion protein will be highlighted. Finally, some of the main strategies that have been used for successful mucosal vaccination of caries vaccine are reviewed, followed by discussion of the mucosal adjuvant choice for achieving protective immunity at oral mucosal membranes for development of a nasal-spray or nasal-drop anti-caries vaccine for human. mutans streptococci, secretory IgA, PAc, mucosal immunization, caries vaccine Citation:Yan H M. Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine.

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Section: Enhancement Of Salivary Siga Antibodies and Anti-caries Protmentioning

confidence: 99%

Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Yan

2013

Sci. China Life Sci.

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“…Therefore, for a multicomponent vaccine containing fHbp, it would be desirable to use a single rfHbp capable of eliciting serum bacteri- Chimeric proteins previously have been used for vaccine development in a variety of ways. One strategy employed a genetic fusion of two different antigens from the same organism to enhance cross-protection against strains with antigenic diversity (18,46). An example is a multicomponent meningococcal group B vaccine that contains two fusion proteins, GNA2091 fused with fHbp v.1 and GNA2132 fused with GNA1030, along with a third antigen, rNadA (17).…”

Section: Discussionmentioning

confidence: 99%

Bactericidal Antibody Responses Induced by Meningococcal Recombinant Chimeric Factor H-Binding Protein Vaccines

Beernink¹,

Granoff²

2008

Infect Immun

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“…Studies of S. mutans infections in mouse oral cavities have been performed by feeding the animals with powdered diet 301 and diet 2000, which contain unnatural amounts of sucrose (1 and 56%, respectively) (13,38,43). In other infection studies, mice were provided with either a 5% sucrose diet or a sucrose-free diet, in which the 56% sucrose in diet 2000 was replaced with wheat flour (15,30).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies of S. mutans infections in the oral cavities of mice have been performed by feeding the animals diets containing sucrose in the presence of glucans (13,15,30,43). Since the adherence of S. mutans to the tooth surface may depend on the balance between physical adherence and synthesis of in- (18,39).…”

mentioning

confidence: 99%

Establishment of an Animal Model Using Recombinant NOD.B10.D2Mice To Study Initial Adhesion of Oral Streptococci

Salam

Matsumoto

Matin

et al. 2004

Clin Vaccine Immunol

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An oral biofilm is a community of surface-attached microorganisms that coats the oral cavity, including the teeth, and provides a protective reservoir for oral microbial pathogens, which are the primary cause of persistent and chronic infectious diseases in patients with dry mouth or Sjögren's syndrome (SS). The purpose of this study was to establish an animal model for studying the initial adhesion of oral streptococci that cause biofilm formation in patients with dry mouth and SS in an attempt to decrease the influence of cariogenic organisms and their substrates. In nonobese diabetogenic (NOD) mice that spontaneously develop insulindependent diabetes mellitus (IDDM) and SS, we replaced major histocompatibility complex (MHC) class II (A g7 E g7 ) and class I D b with MHC class II (A d E d ) and class I D d from nondiabetic B10.D2 mice to produce an animal model that inhibited IDDM without affecting SS. The adhesion of oral streptococci, including Streptococcus mutans, onto tooth surfaces was then investigated and quantified in homologous recombinant N5 (NOD.B10.D2) and N9 (NOD.B10.D2) mice. We found that a higher number of oral streptococci adhered to the tooth surfaces of N5 (NOD.B10.D2) and N9 (NOD.B10.D2) mice than to those of the control C57BL/6 and B10.D2 mice. On the basis of our observation, we concluded that these mouse models might be useful as animal models of dry mouth and SS for in vivo biological studies of oral biofilm formation on the tooth surfaces.

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Enhanced Immunogenicity of a Genetic Chimeric Protein Consisting of Two Virulence Antigens ofStreptococcus mutansand Protection against Infection

Cited by 52 publications

References 48 publications

Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Bactericidal Antibody Responses Induced by Meningococcal Recombinant Chimeric Factor H-Binding Protein Vaccines

Establishment of an Animal Model Using Recombinant NOD.B10.D2Mice To Study Initial Adhesion of Oral Streptococci

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