Enhanced immunogenicity of an anti‐caries vaccine encoding a cell‐surface protein antigen of <i>Streptococcus mutans</i> by intranasal DNA prime‐protein boost immunization

Li, Yuhong; Jin, Jie; Yang, Yaping; Bian, Zhuan; Chen, Zhi; Fan, Mingwen

doi:10.1002/jgm.1386

Cited by 9 publications

(10 citation statements)

References 46 publications

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“…In particular, microand nano-particle delivery systems that encapsulate protein antigens or DNA that encode for antigens have been evaluated. However, the focus of intranasal vaccination has often been on the treatment of infectious diseases 29,[41][42][43][44][45][46][47] . Nonetheless, a recent study has demonstrated that nanoparticles composed of modified c-polyglutamic acid (c-PGA) encapsulating full length OVA protein instilled intranasally induced anti-tumor immunity against melanoma 48 .…”

Section: Discussionmentioning

confidence: 99%

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Phua

Staats

Nair

et al. 2015

Journal of Controlled Release

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Section: Discussionmentioning

confidence: 99%

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Phua

Staats

Nair

et al. 2015

Journal of Controlled Release

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“…In particular, micro- and nano-particle delivery systems that encapsulate protein antigens or DNA that encode for antigens have been evaluated. However, the focus of intranasal vaccination has often been on the treatment of infectious diseases2941424344454647. Nonetheless, a recent study has demonstrated that nanoparticles composed of modified γ-polyglutamic acid (γ-PGA) encapsulating full length OVA protein instilled intranasally induced anti-tumor immunity against melanoma48.…”

Section: Discussionmentioning

confidence: 99%

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Phua

Staats

Leong

et al. 2014

Sci Rep

105

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Direct in vivo administration of messenger RNA (mRNA) delivered in both naked and nanoparticle formats are actively investigated because the use of dendritic cells transfected ex vivo with mRNA for cancer therapy is expensive and needs significant infrastructure. Notably, intravenous and subcutaneous injections are the only routes of administration tested for mRNA nanoparticle tumor vaccination. In this report, we demonstrate that tumor immunity can be achieved via nasal administration of mRNA. Mice nasally immunized with mRNA delivered in nanoparticle format demonstrate delayed tumor progression in both prophylactic and therapeutic immunization models. The observed tumor immunity correlates with splenic antigen-specific CD8+ T cells and is achieved only when mRNA is delivered in nanoparticle but not in naked format. In conclusion, we demonstrate, as a proof-of-concept, a non-invasive approach to mRNA tumor vaccination, increasing its potential as a broadly applicable and off-the-shelf therapy for cancer treatment.

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“…The effect of S-IgA antibodies on the adhesion of S. mutans MT8148 to S-HA beads was examined as previously described [20] . Briefly, 5 mg of HA beads were equilibrated in buffered KCl at room temperature overnight.…”

Section: The Adherence Inhibition Assaymentioning

confidence: 99%

Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro

Huang

Liu

et al. 2012

Acta Pharmacol Sin

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Aim: To investigate the effects of anti-caries DNA vaccine-induced salivary secretory immunoglobulin A (S-IgA) antibodies on Streptococcus mutans (S. mutans) adherence and biofilms formation in vitro. Methods: Adult female Wistar rats were intranasally immunized with the anti-caries DNA vaccine pGJA-P/VAX. Their saliva samples were collected at different times after the immunization, and S-IgA antibody level in the saliva and its inhibition on S. mutans adherence were examined. The effects of S-IgA in the saliva with the strongest inhibitory effects were examined at 3 different stages, ie acquired pellicles, biofilm formation and production of mature biofilms. The number of viable bacteria and depth of the biofilm at 16 h in each stage were determined using counting colony forming units and using a confocal laser scanning microscopy (CLSM). The participation of S-IgA in acquired pellicles and its aggregation with S. mutans were also observed under CLSM. Results: The S-IgA titer in saliva reached its peak and exhibited the strongest inhibition on S. mutans adhesion at 10 weeks after the immunization. The colonies and depth of the biofilm in the saliva-pretreated group were 41.79% and 41.02%, respectively, less than the control group. The colonies and depth of the biofilm in the co-culture group were 27.4% and 22.81% less than the control group. The assembly of S. mutans and S-IgA was observed under CLSM after co-cultivation. In the mature-stage biofilm, no differences were observed between the different groups.Conclusion: These results demonstrate that the anti-caries DNA vaccine induces the production of specific S-IgA antibodies that may prevent dental caries by inhibiting the initial adherence of S. mutans onto tooth surfaces, thereby reducing the accumulation of S. mutans on the acquired pellicles.

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Enhanced immunogenicity of an anti‐caries vaccine encoding a cell‐surface protein antigen of Streptococcus mutans by intranasal DNA prime‐protein boost immunization

Abstract: The results obtained in the present study suggest that the intranasal DNA prime-protein boost vaccination regimen is a novel strategy for the practical application of DNA vaccine against dental caries.

Cited by 9 publications

References 46 publications

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Intranasal mRNA nanoparticle vaccination induces prophylactic and therapeutic anti-tumor immunity

Anti-caries DNA vaccine-induced secretory immunoglobulin A antibodies inhibit formation of Streptococcus mutans biofilms in vitro

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