Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze

Journal of Engineering Technology and Applied Sciences

2022

Tissue culture applications help mass production for many plant species, especially horticultural plants, ornamental plants, medicinal and aromatic plants. Therefore, optimization of production techniques is very important. In this study, the effects of different Murashige and Skoog (MS) salt levels (25%-200%) on the in vitro production of Staurogyne repens (Nees) Kuntze were investigated. The shoot tip was used as explant. Different levels of MS salts significantly affected the regeneration abilities of shoot tip explants. The highest shoot regeneration rate was determined at 100% and 150% MS salt levels. The maximum regenerated shoots (9.13 shoots/explant) was determined in nutrient media supplemented with 150% MS salts. The minimum count of shoots (5.22 shoots/explant) was recorded in food media including 200% MS salts. Mean shoot lengths were between 1.65-2.27 cm. The highest length value (2.27 cm) was determined in the culture medium with 100% MS salts added, while the lowest length value (1.65 cm) was determined in the nutrient medium containing 25% MS salts. The shoots in the propagation medium were rooted in nutrient medium supplemented with 0.25 mg/L indole-3-acetic acid (IAA). Then they were successfully acclimatized to external conditions.

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

Influence of Different Concentrations of Murashige and Skoog Medium on Multiple Shoot Regeneration of Staurogyne repens (Nees) Kuntze

Journal of Engineering Technology and Applied Sciences

2022

“…The plants were surface-sterilized before being cultured. Surface sterilization of the plants was provided according to Kose et al (2021). Then, the shoot tip explants were isolated and placed to glass tubes including MS (Murashige and Skoog 1962).…”

Section: Materıals and Methodsmentioning

confidence: 99%

High Efficiency Plant Regeneration From Shoot Tip Explants Of Staurogyne Repens (Nees) Kuntze

2022

Bangladesh J. Bot.

A method for tissue culture of micropropagation of aquatic plant Staurogyne repens (Nees) Kuntze has been developed. Different phytohormones were investigated to optimize the growth of explants. The shoot tip explants of S. repens were placed in MS nutrient medium including BAP (0.30-1.50 mg/l) + TDZ (0.10 mg/l) + NAA (0.10 mg/l) and Kn (0.30-1.50 mg/l) + TDZ (0.10 mg/l) + NAA (0.10 mg/l). Shoot regeneration frequencies were changed as 77.77-100% and 66.66-100% in cultivation medium augmented with BAPTDZ- NAA and Kn-TDZ-NAA, respectively. In the growing media with BAP, the maximum shoot number (14.44 shoot/explant) was obtained in MS medium with 1.20 mg/l BAP + 0.10 mg/l TDZ + 0.10 mg/l NAA. In Kn applications, the highest shoot number (9.50 shoot/explant) was recorded in growing medium with 0.30 mg/l Kn + 0.10 mg/l TDZ + 0.10 mg/l NAA. The longest shoots (2.33 cm) was reached in nutrient medium with 0.90 mg/l Kn + 0.10 mg/l TDZ + 0.10 mg/l NAA. Regenerated plants rooting at 0.25 mg/l IAA have been effectively acclimated to aquarium conditions. Bangladesh J. Bot. 51(4): 697-704, 2022 (December)

“…ex Roxb) Koehne (Dogan, 2018), Hibiscus coddii subsp. barnardii (Plessis et al, 2021), Echinacea angustifolia (Tyub et al, 2021), Anubias heterophylla (Rittirat et al, 2021), Staurogyne repens (Nees) Kuntze (Kose et al, 2021), Campomanesia phaea (Demétrio et al, 2021) and Lindernia antipoda (L.) Alston (Viella, 2021) by tissue culture methods.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Micropropagation of Pogostemon erectus (Dalzell) Kuntze in Liquid Culture Medium

Natural and Engineering Sciences

2022

Self Cite

Pogostemon erectus (Dalzell) Kuntze is a valuable aquatic-ornamental plant. This study was designed for the multiplication of P. erectus in a liquid culture medium. In this context, shoot tip explants of P. erectus were placed in Murashige and Skoog (MS) food solutions including Zeatin (ZEA: 0.1-2.4 mg/L) and indole acetic acid (IAA: 1.2 mg/L). In addition, experiments were set up in hormone-free environments as controls. In general, high-frequency results were recorded. 100 % regeneration was determined in the treatment of 0.6 mg/L ZEA + 1.2 mg/L IAA. The best results for shoot count and shoot length were obtained with 0.6 mg/L ZEA + 1.2 mg/L IAA and 0.3 mg/L ZEA + 1.2 mg/L IAA, respectively. Generally, low regeneration values were seen in nutrient solutions using high or low doses of ZEA. Shoots were rooted in MS nutrient media with 0.25 mg/L IAA. Then they were transferred to an aquarium and successfully adapted to external conditions.