Enhanced Infrared Neural Stimulation using Localized Surface Plasmon Resonance of Gold Nanorods

Eom, Kyungsik; Kim, Jinhyung; Choi, Jong Min; Kang, Taekyeong; Chang, Jin Woo; Byun, Kyung Min; Jun, Sang Beom; Kim, Sung June

doi:10.1002/smll.201400599

Cited by 151 publications

(152 citation statements)

References 22 publications

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“…In photothermal modulation, however, the relatively high power density of visible or infrared light is needed to manipulate neural activity through either excitation or inhibition. Intense power density (10 W/mm 2 ~10 kW/mm 2 ) with ultrashort pulse width was required for neural excitation [9,19], while moderate power density (10 mW/mm 2 ~30 mW/mm 2 ) with prolonged pulse width was used for neural inhibition [10,11,20]. Despite the promising capability of photothermal methods, spatial patterning of photothermal illumination has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

Jung

Kang

Nam

2017

Biomed. Opt. Express

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Light-mediated neuromodulation techniques provide great advantages to investigate neuroscience due to its high spatial and temporal resolution. To generate a spatial pattern of neural activity, it is necessary to develop a system for patterned-light illumination to a specific area. Digital micromirror device (DMD) based patterned illumination system have been used for neuromodulation due to its simple configuration and design flexibility. In this paper, we developed a patterned near-infrared (NIR) illumination system for region specific photothermal manipulation of neural activity using NIR-sensitive plasmonic gold nanorods (GNRs). The proposed system had high power transmission efficiency for delivering power density up to 19 W/mm 2 . We used a GNR-coated microelectrode array (MEA) to perform biological experiments using E18 rat hippocampal neurons and showed that it was possible to inhibit neural spiking activity of specific area in neural circuits with the patterned NIR illumination. This patterned NIR illumination system can serve as a promising neuromodulation tool to investigate neuroscience in a wide range of physiological and clinical applications. 16 (7), 805-815 (2013). 18. Y. M. Tamar Arens-Arad, N. Faraha, S. Ben-Yaishc, and A. Zlotnikc, "Head mounted DMD based projection system for natural and prosthetic visual stimulation in freely moving rats," Sci.

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Section: Introductionmentioning

confidence: 99%

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

Jung

Kang

Nam

2017

Biomed. Opt. Express

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“…Illuminating gold nanorods (GNRs) at their resonant wavelengths leads to efficient light absorption and local electromagnetic field enhancement, finally realizing a plasmonic nanoheater [22][23][24]. Since plasmonic nanoheaters can efficiently elevate local temperature, INS combined with GNRs has a potential for improving an optical activation or inhibition of neuronal metabolisms [18,[24][25][26] while avoiding tissue damage by excessive heating. Previously, we presented that an optical neural stimulation using NIR light incorporating plasmonic nanoheaters injected in a proximity of neuron cells could significantly enhance the neural responses of a rat sciatic nerve in vivo [24].…”

Section: Introductionmentioning

confidence: 99%

“…Since plasmonic nanoheaters can efficiently elevate local temperature, INS combined with GNRs has a potential for improving an optical activation or inhibition of neuronal metabolisms [18,[24][25][26] while avoiding tissue damage by excessive heating. Previously, we presented that an optical neural stimulation using NIR light incorporating plasmonic nanoheaters injected in a proximity of neuron cells could significantly enhance the neural responses of a rat sciatic nerve in vivo [24]. However, unconjugated nanoparticles might be ineffective when they are washed off by extracellular fluid flow.…”

Section: Introductionmentioning

confidence: 99%

Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation

Eom

Hwang

et al. 2016

Biomed. Opt. Express

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Despite a potential of infrared neural stimulation (INS) for modulating neural activities, INS suffers from limited light confinement and bulk tissue heating. Here, a novel methodology for an advanced optical stimulation is proposed by combining near-infrared (NIR) stimulation with gold nanorods (GNRs) targeted to neuronal cell membrane. We confirmed experimentally that in vitro and in vivo neural activation is associated with a local heat generation based on NIR stimulation and GNRs. Compared with the case of NIR stimulation without an aid of GNRs, combination with celltargeted GNRs allows photothermal stimulation with faster neural response, lower delivered energy, higher stimulation efficiency and stronger behavior change. Since the suggested method can reduce a requisite radiant exposure level and alleviate a concern of tissue damage, it is expected to open up new possibilities for applications to optical neuromodulations for diverse excitable tissues and treatments of neurological disorders. Hollenberg, M.-E. Lee, and E. Haber, "Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines," Circ. Res. 82(8), 845-851 (1998). 37. S. Tandon, N. Kambi, and N. Jain, "Overlapping representations of the neck and whiskers in the rat motor cortex revealed by mapping at different anaesthetic depths," Eur. J. Neurosci. 27(1), 228-237 (2008). 38. M. Brecht, M. Schneider, B. Sakmann, and T. W. Margrie, "Whisker movements evoked by stimulation of single pyramidal cells in rat motor cortex," Nature 427(6976), 704-710 (2004).

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“…live/dead, MTS, MTT, etc.) while histological analysis is often performed during tissue experiments 7,8,12,15 .…”

Section: Discussionmentioning

confidence: 99%

“…The heating produced by the laser-irradiated particles was measured using patch-clamp techniques 14 . More recently, Eom et al recorded an increase in the amplitude of CNAPs after laser exposure of 3.4 × 10 9 Au NRs when continuously perfused in the vicinity of the sheath of the nerve bundle of a rat sciatic nerve 15 .…”

Section: Discussionmentioning

confidence: 99%

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

Paviolo

McArthur

Stoddart

2015

JoVE

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Recent studies have demonstrated that nerves can be stimulated in a variety of ways by the transient heating associated with the absorption of infrared light by water in neuronal tissue. This technique holds great potential for replacing or complementing standard stimulation techniques, due to the potential for increased localization of the stimulus and minimization of mechanical contact with the tissue. However, optical approaches are limited by the inability of visible light to penetrate deep into tissues. Moreover, thermal modelling suggests that cumulative heating effects might be potentially hazardous when multiple stimulus sites or high laser repetition rates are used. The protocol outlined below describes an enhanced approach to the infrared stimulation of neuronal cells. The underlying mechanism is based on the transient heating associated with the optical absorption of gold nanorods, which can cause triggering of neuronal cell differentiation and increased levels of intracellular calcium activity. These results demonstrate that nanoparticle absorbers can enhance and/or replace the process of infrared neural stimulation based on water absorption, with potential for future applications in neural prostheses and cell therapies. Video LinkThe video component of this article can be found at

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Enhanced Infrared Neural Stimulation using Localized Surface Plasmon Resonance of Gold Nanorods

Cited by 151 publications

References 22 publications

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

Synergistic combination of near-infrared irradiation and targeted gold nanoheaters for enhanced photothermal neural stimulation

Gold Nanorod-assisted Optical Stimulation of Neuronal Cells

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