2011
DOI: 10.1007/s12011-011-9092-z
|View full text |Cite
|
Sign up to set email alerts
|

Enhanced Iron and Zinc Accumulation in Genetically Engineered Pineapple Plants Using Soybean Ferritin Gene

Abstract: Pineapple (Ananas comosus L. Merr., cv. "Queen") leaf bases were transformed with Agrobacterium tumefaciens strain EHA 105 harboring the pSF and pEFESF plasmids with soybean ferritin cDNA. Four to eight percent of the co-cultivated leaf bases produced multiple shoots 6 weeks after transfer to Murashige and Skoog's medium supplemented with α-naphthalene acetic acid 1.8 mg/l, indole-3-butyric acid 2.0 mg/l, kinetin 2.0 mg/l, cefotaxime 400 mg/l, and kanamycin 50 mg/l. Putatively transformed shoots (1-2 cm) were … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
5
0
1

Year Published

2012
2012
2022
2022

Publication Types

Select...
5
5

Relationship

0
10

Authors

Journals

citations
Cited by 14 publications
(6 citation statements)
references
References 18 publications
0
5
0
1
Order By: Relevance
“…Iron is also an element that many cultivated soils lack in a bioavailable form, with the World Health Organisation (WHO) estimating that 30% of the world's human population are anaemic. To date, successful attempts have been made to increase the iron and zinc content of rice, pineapple and banana by expression of soybean ferritin (Goto et al, 1999;Kumar et al, 2011;Mhatre et al, 2011). Approaches such as biofortification may also allow the cultivation of additional land; as one common problem that affects land use and crop yield is a lack, or toxic excess, of mineral nutrients (Lal, 2009;Beddington, 2010).…”
Section: Discussionmentioning
confidence: 99%
“…Iron is also an element that many cultivated soils lack in a bioavailable form, with the World Health Organisation (WHO) estimating that 30% of the world's human population are anaemic. To date, successful attempts have been made to increase the iron and zinc content of rice, pineapple and banana by expression of soybean ferritin (Goto et al, 1999;Kumar et al, 2011;Mhatre et al, 2011). Approaches such as biofortification may also allow the cultivation of additional land; as one common problem that affects land use and crop yield is a lack, or toxic excess, of mineral nutrients (Lal, 2009;Beddington, 2010).…”
Section: Discussionmentioning
confidence: 99%
“…But the utilization of this method depicts environmental and economical pressure (Wissuwa et al, 2006), and Zn fertilizers convert to insoluble forms within 7 days of application (Rattan and Shukla, 1991;Hussain et al, 2018). Other methods involve three important approaches that include breeding (Swamy et al, 2016), genetic engineering (Mhatre et al, 2011;Das et al, 2020), and transgenics (Malik and Maqbool, 2020). However, all these approaches are costlier, slower, and laborious (Upadhayay et al, 2019).…”
Section: Introductionmentioning
confidence: 99%
“…Regular crop rotation and intercropping has been used in various areas (Gunes et al, 2007 ; Zuo and Zhang, 2009 ) to promote zinc uptake by plants. Other methods include conventional breeding (Cakmak et al, 2010 ), transgenic approaches and genetic engineering (Gustin et al, 2009 ; Mhatre et al, 2011 ; Tan et al, 2015 ). However, these approaches are expensive, laborious and slower.…”
Section: Introductionmentioning
confidence: 99%