Enhanced killing of Candida albicans by cultured peritoneal exudate cells treated with SM-1213, a synthetic immunomodulator

Morrison, Christine J.; Gordon, P.; Hashimoto, Tadayo

doi:10.1128/aac.26.1.74

Cited by 8 publications

(1 citation statement)

References 15 publications

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“…Quantitative determination of the viability of C. albwcans hyphae damaged by macrophages. The viability of individual C. albicans hyphae exposed to macrophages was determined by the microculture method described earlier (18,22). With this method the ability of viable hyphae to develop buds and form microcolonies could be determined.…”

Section: Preparation Of L Monocytogenes-inmune Macrophagesmentioning

confidence: 99%

In vitro study of contact-mediated killing of Candida albicans hyphae by activated murine peritoneal macrophages in a serum-free medium

Hashimoto

1991

Infect Immun

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Activated peritoneal macrophages obtained from Listeria-immune mice were demonstrated to kill nonphagocytosable Candida albicans hyphae by contact-mediated mechanisms in a serum-free synthetic medium. The actual killing of hyphae was confirmed by a microculture technique utilizing the dimorphic nature of the fungus. The most efficient candidacidal activity was demonstrated by the macrophages obtained from mice first immunized with live Listeria monocytogenes and then elicited with heat-killed L. monocytogenes cells. Resident macrophages from control mice showed only low candidacidal activity against C. albicans hyphae and yeast cells. Direct physical contact appeared to be required for macrophages to efficiently kill oversized C. albicans hyphae. Efficient in vitro killing of hyphae also required relatively high effector/target cell ratios (50 or higher). The contact-mediated candidacidal activity of activated macrophages was not significantly abrogated by oxygen-radical scavengers, suggesting the involvement of oxygen-independent mechanisms. These results suggest that the enhanced nonspecffic immunity to candidiasis seen in Listeria-immune hosts can be attributed, at least in part, to activated fungicidal macrophages. The ability of macrophages to detect and destroy both yeast and hyphal C. albicans cells is clearly an important element of the host defense against candidiasis.

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Section: Preparation Of L Monocytogenes-inmune Macrophagesmentioning

confidence: 99%

In vitro study of contact-mediated killing of Candida albicans hyphae by activated murine peritoneal macrophages in a serum-free medium

Hashimoto

1991

Infect Immun

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Structure of Amiprilose Hydrochloride, a Novel Anti-inflammatory Agent

Linhardt¹,

Baenziger²,

Ronsen

1990

Journal of Pharmaceutical Sciences

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Inhibition of psoriatic cell proliferation in in vitro skin models by amiprilose hydrochloride

Chapman

Dimitrijevich

Hevelone

et al. 1990

In Vitro Cell Dev Biol

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Amiprilose hydrochloride, a 3-substituted glucose derivative, was found to inhibit the proliferation of human fibroblasts and keratinocytes originating from psoriatic lesions. Fibroblasts and keratinocytes were obtained from skin biopsies of normal donors, and from the biopsies of active/involved and uninvolved sites of psoriatic donors. The cells were cultured as monolayers or as components of tissue equivalent models. Keratinocytes and fibroblasts originating from biopsies of psoriatically involved areas were shown to proliferate at a significantly higher rate than those derived from uninvolved areas. The antiproliferative effect of amiprilose hydrochloride was not observed with normal keratinocytes or fibroblasts from the skin of healthy donors or from uninvolved areas of psoriatic donors. Amiprilose hydrochloride was not cytotoxic to any of these cells at levels below 0.1%. The combination of the low cytotoxicity and the selective antiproliferative effect indicates that this compound may be a useful antipsoriatic agent. The use of monolayer cultures and tissue equivalent models in this study illustrates the utility of such a progressive strategy in the evaluation of potential topical pharmaceuticals.

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Enhanced killing of Candida albicans by cultured peritoneal exudate cells treated with SM-1213, a synthetic immunomodulator

Cited by 8 publications

References 15 publications

In vitro study of contact-mediated killing of Candida albicans hyphae by activated murine peritoneal macrophages in a serum-free medium

In vitro study of contact-mediated killing of Candida albicans hyphae by activated murine peritoneal macrophages in a serum-free medium

Structure of Amiprilose Hydrochloride, a Novel Anti-inflammatory Agent

Inhibition of psoriatic cell proliferation in in vitro skin models by amiprilose hydrochloride

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