Enhanced lactic acid production by Bacillus coagulans through simultaneous saccharification, biodetoxification, and fermentation

Abstract

Agricultural straw is abundant worldwide, but efficient production of lactic acid from straw is still challenging. In this paper, Trichoderma viride R16 was cultivated using corncob as the substrate in a fed-batch process, after which the enzymes from T. viride R16 broth were analyzed through protein mass J Liu et al.Spotlight: Dioxygenase from Trichoderma viride R16 enhances the lactic acid production using pretreated corncob spectrometry and used for lactic acid production. Dioxygenase activity was found in the T. viride R16 enzymes, and 0.2 g L -1 phenolics (vanillin, 4-hydroxybenzaldehyde, syringaldehyde) was degraded during enzyme production. Lactic acid production and yield from NH 3 -H 2 O 2 pretreated and washed corncob were increased by more than 24% and total phenolics was significantly lower using T. viride R16 enzymes compared with commercial enzymes in batch and fed-batch fermentation. These characteristics of T. viride R16 support industrial applications with efficient in situ detoxification of phenolic inhibitors without adding an extra step during lactic acid production from pretreated agricultural straw using simultaneous saccharification and fermentation.

Bismuth ferrite Fenton‐like pretreatment improves lactic acid production from corn stover without detoxification by Bacillus coagulans

“…Lactic acid, acetic acid, glucose, and xylose were analyzed using an Agilent 1200 HPLC system (Agilent Technologies, California, USA) equipped with an RID‐10A detector and a Bio‐Rad Aminex HPX‐87H (Hercules, California, USA). The conditions were set as we reported previously 5 . Specifically, the column temperature was kept at 40 °C, the mobile phase was set as 5 mmol L −1 H 2 SO 4 , and the flow rate was 0.6 mL min −1 .…”

“…The conditions were set as we reported previously. 5 Specifically, the column temperature was kept at 40 °C, the mobile phase was set as 5 mmol L −1 H 2 SO 4 , and the flow rate was 0.6 mL min −1 . The concentration of phenolics was determined by the Folin-Ciocalteu method, 27 with gallic acid as the calibration standard.…”

Fermentative Lactic Acid Production From Lignocellulosic Feedstocks: From Source to Purified Product

“…Lactic acid production from 50 g/L ammonia pretreated corn stover was increased nearly twofold [from 16.98 g/L to 31.35 g/L LA (0.63 g/g corn stover)] by inoculating phenolic degrading bacteria mentioned above and lactic acid bacteria Lactobacillus pentosus FL0421 . In another paper, Liu et al (2020) cultivated Trichoderma viride R16 on alkaline/peroxide pretreated corncob as the substrate in a fed-batch SSF process, and the produced enzymes were used in LA production by Bacillus coagulans LA204. Because of the high capacity of inhibitors' degradation by Trichoderma viride R16 enzymes compared to some commercial ones the lactic acid production was increased by 24% (Liu et al, 2020).…”

“…In another paper, Liu et al (2020) cultivated Trichoderma viride R16 on alkaline/peroxide pretreated corncob as the substrate in a fed-batch SSF process, and the produced enzymes were used in LA production by Bacillus coagulans LA204. Because of the high capacity of inhibitors' degradation by Trichoderma viride R16 enzymes compared to some commercial ones the lactic acid production was increased by 24% (Liu et al, 2020). Giacon et al (2021) have studied the influence of two inhibitors furfural and HMF on the growth of five homofermentative (Lactobacillus plantarum CECT 221, Lactobacillus delbrueckii, Lactobacillus plantarum ESALQ 4, Lactobacillus paracasei LAB 4, Lactobacillus paracasei LAB 5) and seven (Lactobacillus fermentum DSM 20391, Lactobacillus reuteri ATCC 23272, Lactobacillus fermentum ESALQ 3, Lactobacillus fermentum ESALQ 5, Lactobacillus fermentum 1L-6-MRS, Lactobacillus fermentum 3L-2-M17, Lactobacillus paracasei LAB 2) heterofermentative lactic acid bacteria and have found that the effect of HMF and furfural on the growth rate of lactic acid bacteria (LAB) depended on the metabolic pathway.…”

Production of Lactic Acid from Carob, Banana and Sugarcane Lignocellulose Biomass

“…The numerous applications of LA have made it one of the most important products and its biotechnological production includes integration of pretreatment processes, enzymatic hydrolysis and fermentation. The scaled up processes are aimed at decreasing energy consumption and costs without affecting LA purity [9][10][11].…”