Enhanced lipid and biomass production by a newly isolated and identified marine microalga

Abstract

BackgroundThe increasing demand for microalgae lipids as an alternative to fish has encouraged researchers to explore oleaginous microalgae for food uses. In this context, optimization of growth and lipid production by the marine oleaginous V2-strain-microalgae is of great interest as it contains large amounts of mono-unsaturated (MUFAs) and poly-unsaturated fatty acids (PUFAs).MethodsIn this study, the isolated V2 strain was identified based on 23S rRNA gene. Growth and lipid production conditions were optimized by using the response surface methodology in order to maximize its cell growth and lipid content that was quantified by both flow cytometry and the gravimetric method. The intracellular lipid bodies were detected after staining with Nile red by epifluorescence microscopy. The fatty acid profile of optimal culture conditions was determined by gas chromatography coupled to a flame ionization detector.ResultsThe phenotypic and phylogenetic analyses showed that the strain V2 was affiliated to Tetraselmis genus. The marine microalga is known as an interesting oleaginous species according to its high lipid production and its fatty acid composition. The optimization process showed that maximum cell abundance was achieved under the following conditions: pH: 7, salinity: 30 and photosynthetic light intensity (PAR): 133 μmol photons.m−2.s−1. In addition, the highest lipid content (49 ± 2.1% dry weight) was obtained at pH: 7, salinity: 37.23 and photosynthetic light intensity (PAR): 188 μmol photons.m−2.s−1. The fatty acid profile revealed the presence of 39.2% and 16.1% of total fatty acids of mono-unsaturated fatty acids (MUFAs) and poly-unsaturated fatty acids (PUFAs), respectively. Omega 3 (ω3), omega 6 (ω6) and omega 9 (ω9) represented 5.28%, 8.12% and 32.8% of total fatty acids, respectively.ConclusionsThis study showed the successful optimization of salinity, light intensity and pH for highest growth, lipid production and a good fatty acid composition, making strain V2 highly suitable for food and nutraceutical applications.

Matrix solid-phase dispersion as a greener alternative to obtain bioactive extracts from Haematococcus pluvialis. Characterization by UHPLC-QToF

“…These acids are common in several families of microalgae as in H. pluvialis red. 30,38 Through untargeted analysis, as shown in Table 5, three interesting compounds, which were not found to be reported prior to this study, were identied in H. pluvialis. N-phenyldecanoic acid was identied by both positive and negative ionization, presenting a mass error of less than 1 ppm and a difference in its isotopic pattern of less than 10 mSigma.…”

Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production

“…Flasks were incubated at 28 °C at a continuous illumination intensity (80 μmol photons·m −2 ·s −1 ) provided by cool-white fluorescence tubes (TL5 tungsten filament lamps; Philips Co., Taipei, Taiwan). Every two days, the cultures were visually examined and the algal development was confirmed by inverted microscopy analysis at 40× magnification (Motic microscope AE2000, Barcelona, Spain) [ 49 ].…”

“…The amount of carotenoids ( Y ) was fixed as the design experiments response which could be described by a second order polynomial function. Nemrod-W ® software (LPRAI, Marseille, France) was used, for the experimental design, regression and statistical analysis [ 49 , 53 ] (Logiciel Nemrod-W, LPRAI, Marseille, France). Table 1 and Table 2 show independents variables of the Box–Behnken plan in coded levels where Y is the response variable, β 0 the constant, X i and X j the coded variables ranging between +1 and −1.…”

Biodegradation and detoxification of bisphenol A by bacteria isolated from desert soils

“…The marine microalgae Tetraselmis sp. strainV2 (Dammak et al 2016) was used in this study. In order to evaluate to median effective concentration of BPA after 96 h of incubation EC 50 (96 h), algal cultures supplemented with different concentrations of BPA (0, 0.2, 1, 2.5 and 5 mg L −1 ) were monitored.…”