Enhanced liver functions of hepatocytes cocultured with NIH 3T3 in the alginate/galactosylated chitosan scaffold

Seo, Seog-Jin; Kim, In-Yong; Choi, Yun‐Jaie; Akaike, Toshihiro; Cho, Chong‐Su

doi:10.1016/j.biomaterials.2005.09.018

Cited by 117 publications

(55 citation statements)

References 41 publications

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“…For such a purpose, the biocompatibility of this naturally occurring nanomaterial was thoroughly assessed. Several cell types that are commonly used for bone/liver/ vascular tissue engineering were used to test the cytotoxicity of sundew adhesive, including two types of fibroblast cells, MC3T3 mouse pre-osteoblastic cells [48] and NIH3T3 mouse embryo fibroblast cells [49], as well as two types of smooth muscle cells, MSMCs and RSMCs [50]. As shown in figure 6a, apparent cytotoxic activity was not observed in sundew adhesive against these four types of cells and even at a high concentration of sundew adhesive up to 1% (v/v), the inhibition ratios were still less than 8% for all types of cells tested in this study.…”

Section: Sundew Adhesive: Biocompatible Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Sundew adhesive: a naturally occurring hydrogel

Huang

Wang

Sun

et al. 2015

J. R. Soc. Interface.

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Bioadhesives have drawn increasing interest in recent years, owing to their ecofriendly, biocompatible and biodegradable nature. As a typical bioadhesive, sticky exudate observed on the stalked glands of sundew plants aids in the capture of insects and this viscoelastic adhesive has triggered extensive interests in revealing the implied adhesion mechanisms. Despite the significant progress that has been made, the structural traits of the sundew adhesive, especially the morphological characteristics in nanoscale, which may give rise to the viscous and elastic properties of this mucilage, remain unclear. Here, we show that the sundew adhesive is a naturally occurring hydrogel, consisting of nanonetwork architectures assembled with polysaccharides. The assembly process of the polysaccharides in this hydrogel is proposed to be driven by electrostatic interactions mediated with divalent cations. Negatively charged nanoparticles, with an average diameter of 231.9 + 14.8 nm, are also obtained from this hydrogel and these nanoparticles are presumed to exert vital roles in the assembly of the nano-networks. Further characterization via atomic force microscopy indicates that the stretching deformation of the sundew adhesive is associated with the flexibility of its fibrous architectures. It is also observed that the adhesion strength of the sundew adhesive is susceptible to low temperatures. Both elasticity and adhesion strength of the sundew adhesive reduce in response to lowering the ambient temperature. The feasibility of applying sundew adhesive for tissue engineering is subsequently explored in this study. Results show that the fibrous scaffolds obtained from sundew adhesive are capable of increasing the adhesion of multiple types of cells, including fibroblast cells and smooth muscle cells, a property that results from the enhanced adsorption of serum proteins. In addition, in light of the weak cytotoxic activity exhibited by these scaffolds towards a variety of mammal cells, evidence is sufficient to propose that sundew adhesive is a promising nanomaterial worth further exploitation in the field of tissue engineering.

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Section: Sundew Adhesive: Biocompatible Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Sundew adhesive: a naturally occurring hydrogel

Huang

Wang

Sun

et al. 2015

J. R. Soc. Interface.

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“…Due to its relative stability, biocompatibility, adjustable porosity and simplicity of use, alginate is thus a biomaterial of choice when it comes to entrapping cells (Garbayo et al, 2002;Zmora et al, 2002;David et al, 2004a;De Vos et al, 2006;Zimmermann et al, 2007;Wikstrom et al, 2008), to cell therapy (Chang, 2005;Paul et al, 2009) or to being used in medical devices (Ueyama et al, 2002;Orive et al, 2004;Gao et al, 2005;De Vos et al, 2006;Orive et al, 2006;Wikstrom et al, 2008). Some of these studies were conducted with hepatocytes that were either encapsulated within alginate beads (Selden et al, 1999;David et al, 2004b;Gao et al, 2005;Kinasiewicz et al, 2007;Kinasiewicz et al, 2008) or capsules (Canaple et al, 2001;Orive et al, 2004;Haque et al, 2005), or seeded within alginate scaffolds (Zmora et al, 2002;Seo et al, 2006). More specifically, the effects of the type of alginate on human hepatocyte cell line cultures were studied by Khalil et al (2001).…”

Section: Introductionmentioning

confidence: 99%

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Gautier

Carpentier²,

Dufresne³

et al. 2011

eCM

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Liver-assist devices have been developed in the last few decades to support patients with liver failure on the road to recovery or transplantation. Fluidised bed bio-artificial livers -where liver cells are encapsulated within alginate beads -appear to be a valuable alternative to hollow fibre devices for improving mass transfers and enhancing treatment efficacy. This approach nevertheless deserves optimization in terms of bead production. The aim of this study was to investigate the impact of alginate type and of two bead diameters (1000 μm and 600 μm) on mass transfers within beads and on the biological functions of encapsulated C3A cells.After assessing the effect of the encapsulation process on bead quality, we investigated cell viability and metabolic activities (ammonia, albumin, α-foetoprotein synthesis and glucose consumption). They were successfully maintained over 48 h within fluidised bed bioreactors, independently of alginate type and bead diameter. Mass transfers were not significantly influenced by the latter parameters. Finally, suggestions are made for improving the entrapment process as a means of enhancing the treatment efficiency of the fluidised bed bioartificial liver.

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“…Coculture of rat hepatocytes with pancreatic islets (41) and rat hepatocytes with NIH/3T3 fibroblasts (42,43) enhanced spheroid formation and functions of hepatocytes.…”

Section: Coculture Systemmentioning

confidence: 99%

Roles of Spheroid Formation of Hepatocytes in Liver Tissue Engineering

Jiang¹,

Kim²,

Cho³

et al. 2010

Int J Stem Cells

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The liver plays an important role in a broad spectrum of physiological functions and detoxifies endogenous and exogenous substances. The liver failure is associated with a high risk of mortality because it is one of important organs in our body. Various bioartificial liver (BAL) systems have been used for clinical trials as a bridge for liver transplantations in patients with liver failure. Long term and stable liver-specific functions of hepatocytes in the development of BAL support systems should be considered. Spheroid formation of hepatocytes enhances liver-specific functions. In this review, hepatocyte spheroid formation methods such as galactose density, topology of extracellular matrix, micromolding technique, hanging-drop culture, non-adhesive surface, positive charged surface, spinner culture, rocked technique, medium component, external forces, coculture system and polymeric nanospheres are explained for enhancing liver-specific functions.

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Enhanced liver functions of hepatocytes cocultured with NIH 3T3 in the alginate/galactosylated chitosan scaffold

Cited by 117 publications

References 41 publications

Sundew adhesive: a naturally occurring hydrogel

Sundew adhesive: a naturally occurring hydrogel

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Roles of Spheroid Formation of Hepatocytes in Liver Tissue Engineering

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