Enhanced long-term ammonium removal and its ranked contribution of microbial genes associated with nitrogen cycling in a lab-scale multimedia biofilter

Microbial nitrogen removal pathways in integrated vertical-flow constructed wetland systems

“…The copy numbers of anammox 16S rRNA in the downflow and up-flow columns were 3.51 Â 10 7 and 3.38 Â 10 7 in W1; and 5.58 Â 10 7 and 4.38 Â 10 7 in W2, respectively. These copy numbers in our IVCWs were relatively higher than those reported in the previous studies elsewhere (Wang and Li, 2011;Wang et al, 2015), such as HSSF-CWs, TFCWs, and multimedia biofilters. This is probably due to the predominantly anaerobic conditions in the IVCWs.…”

Review on the Fate and Mechanism of Nitrogen Pollutant Removal from Wastewater Using a Biological Filter

“…The nitrite oxidase coding gene nxrA has been utilized as a marker of NO 2 --N oxidation [41]. Wang et al [1] reported that the functional roles of AOB, AOA, and The nitrification process was determined to be a pivotal pathway responsible for the robust NH 4 + -N treatment performance in biofilters. The primary factors in controlling nitrification process include free ammonia, temperature, dissolved oxygen (DO), and pH.…”

“…Archaeal ammonium-oxidizer amoA sequences have been identified in a laboratory-scale reactor [59]. Wang et al [1] investigated the dynamic populations of Archaea in a lab-scale multimedia biofilter. The results suggested that Archaeal showed a much lower abundance (relative to bacteria).…”

“…Due to the different responses of nitrogen microorganisms to operating parameters (free ammonia concentration, temperature, dissolved oxygen (DO), and pH, etc.) and the complex interrelationships among various nitrogen species, achieving satisfactory nitrogen removal in biofilters remains a challenge [1,15]. Thus, understanding the underlying microbial nitrogen transformation mechanisms under different operating conditions has extended concurrently with the use of biofilters.…”

“…Biofilters afford many considerable advantages, including simple design and operation, low capital and operating costs, and a low requirement for energy and maintenance inputs [7]. During the past two decades, use of biofilter systems has developed rapidly, and biofilters have been widely used to remove various pollutants originating from aquatic environments [1,[8][9][10][11][12].…”