Enhanced mass resolution in matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry

Schuerch, S.; Schaer, Martin; Boernsen, K. Olaf; Schlunegger, U. P.

doi:10.1002/bms.1200231108

Cited by 10 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These experiments require long time stability, and cannot be performed with solid matrices. Collision cross sections have been measured in previous MALDI experiments;21–23 the main interest of that work were effects of the background gas on the distortion of TOF spectra. In gas‐phase experiments the structural changes of [C 7 H 7 ] + due to isomerization have been studied 19.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A comparative study of a liquid and a solid matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and collision cross section measurements

Ring

Rudich

2000

Rapid Commun. Mass Spectrom.

View full text Add to dashboard Cite

We present experimental matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) results comparing a liquid (glycerol/K(4)[Fe(CN)(6)]) and a solid matrix (2,5-dihydroxybenzoic acid, DHB) with respect to analyte signal stability and initial ion velocity. For applications requiring stable production of analyte ions over a long period of time, the liquid matrix is superior to the solid matrix. The stable analyte ion signal obtained from a liquid matrix allowed the measurement of collision cross sections of small poly(ethylene glycol) (PEG(n)) adduct ions in the flight tube with good resolution. The initial velocity of these adduct ions was measured. It was found that analyte molecules from the liquid matrix have initial ion velocities significantly smaller than those from the solid matrix. MALDI-TOF measurements for large molecules using a liquid matrix are therefore likely to result in smaller systematic errors in mass calibrations due to initial ion velocity.

show abstract

Section: Resultsmentioning

confidence: 99%

“…20 This however is difficult, considering the short lifetime and the low reproducibility of common MALDI ion sources. Only a few studies on cross section measurements in MALDI instruments exist, and they were mainly directed at probing the effect of pressure on the MALDI spectra 21–23…”

mentioning

confidence: 99%

A comparative study of a liquid and a solid matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and collision cross section measurements

Ring

Rudich

2000

Rapid Commun. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…The last process would seem to be the most probable due to the large energy of the ions in the drift tube (-4 keY per charge) and for the same reason the cleav-J Am Soc Mass Spectrom 1996, 7, 342-349 Figure 3. ESI mass spectra of horse heart cytochrome c (l2,3~0 u) recorded at two different pressures in the vacuum chamber in the reflecting mode: (a) p = 0.8 X 10-6 torr; (b) p -4.1 X 10- 6 torr. Each spectrum was recorded in 1 min.…”

Section: Resultsmentioning

confidence: 99%

“…A strong pressure dependence was demonstrated recently for MALO! [6], where ions are ejected directly into vacuum. The influence of pressure can be even more relevant in ESI: ions enter the vacuum chamber from atmosphere through a series of orifices, so there is always a compromise between better vacuum and better sensitivity.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of ion-molecule collisions in the vacuum chamber of an electrospray time-of-flight mass spectrometer on mass spectra of proteins

Chernushevichfn

Verentchikovfn

Ens

et al. 1996

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Electrospray ionization spectra of positive multiply charged ions of several proteins with molecular weight from 8565 to 339,100 u were recorded at different pressures of residual gas in the vacuum chamber of an electrospray time-of-flight mass spectrometer. The pressure was varied in the range (0.2 to 5) × 10(-6) torr. The effect of pressure was found to be significant even for the lightest protein investigated (ubiquitin), which resulted in a decrease of both sensitivity and resolution. Investigations of the arrival-time distributions and the energy distributions of ions showed that collision-induced dissociation (CID) of the protein ions in the drift region is the main process responsible for the effect. Several CID cross sections of proteins were estimated from a series of mass spectra recorded at different pressures in the reflecting mode: 1150 Å(2) for cytochrome c (averaged over charge states z = 14-18), 800 Å(2) for lysozyme (z = 8-10), 1840 Å(2) for apomyoglobin (z = 12-25), 800 Å(2) for holomyoglobin (z = 8), and 2500 Å(2) for carbonic anhydrase II (z = 22-35). Several experiments with large proteins in their native conformations and low charge states (m/z 0,000) demonstrate that these ions are less sensitive to high residual gas pressure.

show abstract

Time‐of‐Flight Mass Spectrometry

Weinberger¹,

Davis

Makarov

et al. 2000

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

This article reviews the developmental history, fundamental technology, and general applications of time‐of‐flight mass spectrometry (TOFMS). The fundamentals of instrument operation and components are discussed. Applications in terms of gas chromatography (GC), liquid chromatography (LC), capillary electrophoresis (CE), laser desorption, multiphoton desorption, plasma desorption (PD), matrix‐assisted laser desorption, surface‐enhanced laser desorption, inductively coupled plasma (ICP), and secondary ion mass spectrometry (SIMS) are presented. A review of tandem time‐of‐flight (TOF) techniques with a focus on ion trap TOF instrumentation is provided. The purpose of this article is to provide the reader with a fundamental understanding of TOFMS principles and applications. Information is presented in a simple, straightforward, didactic fashion.

show abstract

Enhanced mass resolution in matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry

Cited by 10 publications

References 23 publications

A comparative study of a liquid and a solid matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and collision cross section measurements

A comparative study of a liquid and a solid matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and collision cross section measurements

Effect of ion-molecule collisions in the vacuum chamber of an electrospray time-of-flight mass spectrometer on mass spectra of proteins

Time‐of‐Flight Mass Spectrometry

Contact Info

Product

Resources

About