Enhanced matrix production by cocultivated human stem cells and chondrocytes under concurrent mechanical strain

Abusharkh, Haneen A.; Mallah, Alia; Amr, Mahmoud; Mendenhall, Juana; Gozen, B. Arda; Tingstad, Edwin M.; Abu-Lail, Nehal I.; Wie, Bernard J. Van

doi:10.1007/s11626-021-00592-4

Cited by 4 publications

(12 citation statements)

References 45 publications

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“…However, TGF-β is also a common inducing factor of osteogenesis; making it extremely hard to control the fate of stem cells (Chen et al 2012). Finally, co-cultures of chondrocytes and stem cells have also been proposed with the idea that chondrocytes assess stem cells in committing a chondrogenic lineage while stem cells help chondrocytes in proliferating (Acharya et al 2012;Abusharkh et al 2021). The ratio at which both cell types are to be mixed at is yet to be determined.…”

Section: Cellsmentioning

confidence: 99%

“…Equally important to the choice of cells is the choice and design of a scaffold to which cells grow on. Scaffolds should be biocompatible such as they do not elicit foreign immune responses, biodegradable/bioresorbable to be replaced by ECM formed upon implantation, porous to allow for diffusion of nutrients, mechanically sound to support cellular proliferation and differentiation to produce ECM, have appropriate chemistry to enable cell adhesion and growth, and not cytotoxic (Bertrand and Hellmich 2009;Amr et al 2021). Scaffolds used in AC tissue engineering can be divided into natural and synthetic scaffolds.…”

Section: Scaffoldsmentioning

confidence: 99%

“…Based on their locations in the body, the majority of AC tissues are subject to significant mechanical stresses including compression, oscillating hydrostatic pressures (OHP), tensile stresses and shear forces (Bachrach et al 1998, Guilak and Mow 2000, Abusharkh et al 2021, Abusharkh et al 2022. Furthermore, AC tissues are subject to chemical stresses including hypoxia (Lafont 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Compared to other body tissues, AC has least cell density (Wong and Carter 2003). The tissue gains its white color from deprivation of blood (Amr et al 2020;Mallah et al 2021). Finally, the tissue is devoid of nerves and lymph nodes (Fox et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…There currently exists no therapies for OA. The disease is largely managed using uptake of oral non-steroidal anti-inflammatory drugs (NSAIDs) to kill the pain, anti-inflammatory or hyaluronic acid injections, topical medications, physical therapy, weight loss, use of structural support devices or braces, use of comparative alternative medicinal drugs or nutraceuticals and eventually, a total knee replacement (TKR) surgery is needed (Chen et al 2017;Amr et al 2020;Mallah et al 2021). A TKR takes place every minute in the US (Shmerling 2018).…”

Section: Introductionmentioning

confidence: 99%

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Tissue Engineering of Articular Cartilage

Lail

2022

AQUJ

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Articular cartilage (AC) is the tissue that wraps moving joints in the body. As a tissue, it provides lubrication as well as load bearing in some joints. The tissue is subject to harsh chemical and mechanical environments in vivo and is characterized by being devoid of blood, nerve and lymph nodes. Upon injury, the tissue is incapable of healing. Damaged AC tissues mark the global disease of osteoarthritis (OA). As a pandemic, OA is prevalent worldwide and is ranked first for which patients seek treatments. Unfortunately, the disease has no current disease modifying drugs and it is only managed for symptomatic relief using pain killers, physical therapy and surgical procedures. In search for less invasive treatments, tissue engineering has been sought to provide the alternative. Tissue engineering refers to creation of eventually personalized tissues that can be used to replace damaged tissues in vitro. As an approach, it relies on seeding cells on scaffolds and incorporating the two in bioreactors that mimic the joint environment. Cells are then fed a medium with growth factors in it for a period of time until they form tissues. The tissues are then characterized to check if they represent the native tissue in structure and function. If not, the parameters used to engineer the tissue are revisited and the loop is repeated again. Here, tissue engineering of AC will be introduced. After that, the four pillars of tissue engineering (cells, scaffolds, growth factors, and bioreactors) will be discussed with questions that remain to be addressed. A section that discusses how engineered tissues are characterized will follow. Finally, the review ends with a summary of where the field is heading to realized quality AC tissues. This review is not meant to be comprehensive of existing literature.

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Section: Cellsmentioning

confidence: 99%

Section: Scaffoldsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tissue Engineering of Articular Cartilage

Lail

2022

AQUJ

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Combinatorial extracellular matrix cues with mechanical strain induce differential effects on myogenesis in vitro

et al. 2023

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The Influence of Intervertebral Disc Microenvironment on the Biological Behavior of Engrafted Mesenchymal Stem Cells

Zhang

Sun

et al. 2022

Stem Cells International

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Intervertebral disc degeneration is the main cause of low back pain. Traditional treatment methods cannot repair degenerated intervertebral disc tissue. The emergence of stem cell therapy makes it possible to regenerate and repair degenerated intervertebral disc tissue. At present, mesenchymal stem cells are the most studied, and different types of mesenchymal stem cells have their own characteristics. However, due to the harsh and complex internal microenvironment of the intervertebral disc, it will affect the biological behaviors of the implanted mesenchymal stem cells, such as viability, proliferation, migration, and chondrogenic differentiation, thereby affecting the therapeutic effect. This review is aimed at summarizing the influence of each intervertebral disc microenvironmental factor on the biological behavior of mesenchymal stem cells, so as to provide new ideas for using tissue engineering technology to assist stem cells to overcome the influence of the microenvironment in the future.

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Enhanced matrix production by cocultivated human stem cells and chondrocytes under concurrent mechanical strain

Cited by 4 publications

References 45 publications

Tissue Engineering of Articular Cartilage

Tissue Engineering of Articular Cartilage

Combinatorial extracellular matrix cues with mechanical strain induce differential effects on myogenesis in vitro

The Influence of Intervertebral Disc Microenvironment on the Biological Behavior of Engrafted Mesenchymal Stem Cells

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