Enhanced MC3T3 preosteoblast viability and adhesion on polyelectrolyte multilayer films composed of glycol‐modified chitosan and hyaluronic acid

Abstract

Layer-by-layer polyelectrolyte films made of the naturally derived polysaccharides chitosan (CHI) and hyaluronic acid (HA) constitute a well-studied system for the development of cell-responsive biointerfaces. However, many cell lines exhibit decreased adhesion to CHI/HA multilayer films, particularly as the number of bilayers is increased. Here, our group demonstrates that films composed of glycol-modified chitosan exhibit significantly improved MC3T3 preosteoblast adhesion and viability compared to corresponding films consisting of unmodified CHI. These differences in cellular adhesion are likely due to differences in surface topography and roughness, as measured via atomic force microscopy (AFM), as well as in film chemistry and the water solubility of the cation, since both types of films exhibited similar: thickness, as measured via quartz crystal microbalance and AFM; wettability, as measured via contact angle; and serum protein adsorption, as measured via the bicinchoninic acid assay.

Biosynthetic Polymers as Functional Materials

“…147,148 The Hammond Lab has made considerable progress with these materials for temporary protection and variable-timed release of therapeutic cargos via diffusion, stimuli-response, and/or network degradation. 149−153 For instance, naturally derived LbL films were assembled through electrostatic complexation between anionic poly(β- l -malic acid) and cationic chitosan in aqueous conditions.…”

Chitin and Chitosan Relative to Collagen and Hyaluronan

“…Holmes and Trabizian prepared alternate layers of Gly-C 43 and HA by deposition in their study of MC3T3 preosteoblast viability and adhesion on chitosan-HA films [47]. Films with five or more bilayers of Gly-C/HA displayed high cells adherence and viability attributed to surface topography, roughness, and chemistry characteristics that could be useful in drug and gene delivery and TE applications.…”

Mesoporous magnesium silicate-incorporated poly(ε-caprolactone)-poly(ethylene glycol)- poly(ε-caprolactone) bioactive composite beneficial to osteoblast behaviors

“…The hydrophilicity of the biomaterial is an important factor for cell adhesion, and thus improved surface hydrophilicity is beneficial to the interactions between biomaterials and cells. 19 For the m-MPC, the results showed that the water contact angle of the m-MPC decreased from 56° to 0° with the increase of m-MS from 0 w% to 40 w% in the composite, indicating that the m-MS content had significant effects on the hydrophilicity of the m-MPC. The remarkable improvement in hydrophilicity of the m-MPC through incorporation of m-MS into PCL-PEG-PCL suggests that the addition of inorganic bioactive material into polymer is a good way to enhance the hydrophilicity.…”