Enhanced mechanical performances and bioactivity of cell laden-graphene oxide/alginate hydrogels open new scenario for articular tissue engineering applications

Marrella, Alessandra; Lagazzo, Alberto; Barberis, Fabrizio; Catelani, Tiziano; Quarto, Rodolfo; Scaglione, Silvia

doi:10.1016/j.carbon.2017.01.037

Cited by 78 publications

(46 citation statements)

References 52 publications

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“…When the GO concentration was increased from 0.5 to 2 mg/ml in the composite hydrogels, more GO flakes could be observed under the optical microscopy images ( Figure 1E-H). Our result is similar to previous findings reported by Guo and Scaglione et al [17,21] , who demonstrated that the negative ions in both alginate and GO allow the formation of a homogeneous and stable solution.…”

Section: Go Characterization Bioink Preparationsupporting

confidence: 93%

“…They demonstrated that GO-GelMA hybrid hydrogels supported the spreading and alignment of fibroblasts with improved viability and proliferation. Marrella et al [17] confirmed an absence of cytotoxicity of suspended GO flakes and improved viability of fibroblasts encapsulated in 3D GO/alginate hydrogels. Because of the negative surface charge and excellent absorbing properties of GO [20] , we hypothesized that the incorporation of GO into the alginate/gelatin ink system improves scaffold fidelity due to the attractive electrostatic forces between GO and Ca 2+ .…”

Section: Introductionmentioning

confidence: 91%

“…The compressive modulus on day 42 was 12 times higher for the 0GO and 0.5GO scaffolds, 7.3 times higher for the 1GO scaffolds and 4.3 times higher for the 2GO scaffolds than on day 7. Marrella et al [17] also demonstrated that stiffness of GO/alginate functionalized hydrogels was increased dramatically from one…”

Section: Scaffold Mechanicsmentioning

confidence: 97%

“…2D studies have shown that GO improves hMSC adhesiveness and proliferation [15] , cell growth [16] and osteogenic differentiation [16c] . In 3D studies, many researchers have incorporated GO into hydrogel scaffolds, such as alginate [17] , collagen [18] and gelatin methacrylate (GelMA) [19] , and polymer chains were crosslinked to form a 3D GO hybrid scaffold. For example, Su et al [19] incorporated GO into GelMA for the creation of cell-laden graphene-embedding hydrogels and investigated the cellular responses in a 3D microenvironment.…”

Section: Introductionmentioning

confidence: 99%

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3D Bioprinting of Graphene Oxide-Incorporated Cell-laden Bone Mimicking Scaffolds for Promoting Scaffold Fidelity, Osteogenic Differentiation and Mineralization

Zhang

Eyisoylu

Qin

et al. 2020

Preprint

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Bioprinting is a promising technique for facilitating the fabrication of engineered bone tissues for patient-specific defect repair and for developing in vitro tissue/organ models for ex vivo tests. However, polymer-based ink materials often result in insuﬃcient mechanical strength, low scaffold ﬁdelity and loss of osteogenesis induction because of the intrinsic swelling/shrinking and bioinert properties of most polymeric hydrogels. In this work, we developed a novel human mesenchymal stem cell (hMSC)-laden graphene oxide (GO)/alginate/gelatin composite bioink to form 3D bone mimicking scaffolds. Our results showed that the GO composite bioinks with higher GO concentrations improved the bioprintability, scaffold fidelity, compressive modulus and cell viability. The higher GO concentration increased the cell body size and DNA content. The 1GO group had the highest osteogenic differentiation of hMSC with the upregulation of osteogenic-related gene expression at day 42. To mimic critical-sized calvarial bone defects in mice, 3D cell-laden GO defect scaffolds with complex geometries were successfully bioprinted. 1GO maintained the best scaffold fidelity and had the highest mineral volume after culturing in the bioreactor for 42 days. Finally, the 1GO bioink has been demonstrated great potential for 3D bioprinting in applications of bone model and bone tissue engineering.

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Section: Go Characterization Bioink Preparationsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 91%

Section: Scaffold Mechanicsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

3D Bioprinting of Graphene Oxide-Incorporated Cell-laden Bone Mimicking Scaffolds for Promoting Scaffold Fidelity, Osteogenic Differentiation and Mineralization

Zhang

Eyisoylu

Qin

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…In this study, amine-functionalized CNT has been incorporated in the nanocomposites, and as reported in previous studies [49,50], the introduction of carbon derivative materials in extracellular matrix-derived substrate enhanced the cellular proliferation and adhesion. For 9 Journal of Nanomaterials alginate-graphene oxide matrices, it has been reported that the graphene oxide incorporation enhanced the cell viability [51]. These results show that the nanocomposite scaffolds in this study are nontoxic and have a potential application as biomaterial.…”

Section: Journal Of Nanomaterialssupporting

confidence: 58%

Synthesis and Physicochemical Characterization of Multiwalled Carbon Nanotubes/Hydroxamic Alginate Nanocomposite Scaffolds

Lima

Assis

et al. 2018

Journal of Nanomaterials

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In this study, the preparation of porous nanocomposite scaffolds (HX-CNT) from a combination of a hydroxamic derivative of alginate (HX) and an amine-functionalized multiwalled carbon nanotube (CNT) at different concentrations is described. The structure of HX was investigated by FTIR, and the degree of substitution around 9% was confirmed by elemental analysis. The interaction between CNT and alginate derivative in the nanocomposite crosslinked with calcium was confirmed by FTIR, Raman spectroscopy, and SEM. The results obtained in this study showed that scaffolds based on HX-CNT composites with a 4 wt% concentration level exhibited improved physical and mechanical properties compared to plain alginate (Young’s modulus increased from 2.2 to 5.1 MPa and elastic strength from 0.13 to 0.25 MPa) and decreased the swelling ratios from ~900 to ~673. The cytotoxicity assays using the L929 cell line proved that the nanocomposite scaffolds were nontoxic, even at the highest CNT concentration.

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Biodegradable Polymers for Biomedical and Tissue Engineering

Pushpamalar

Paramasivam

Meganathan

et al. 2020

Materials Science and Technology

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Currently, scientists are on the cutting edge of inventing many novel biomaterials from various sources for selection in tissue engineering and controlled drug delivery systems to improvise the patient surgical and treatment processes. This article focuses on the significant improvements made in the design of natural, biodegradable polymer‐based biomaterials and their applications in biomedical and tissue engineering areas. Polysaccharide and protein‐based hydrogels are suitable for soft and hard biomaterials. The soft biomaterial is made of biodegradable natural or synthetic or in a combination of both polymers that can be utilized for a stipulated time and cleared partly or whole of the system that it treats, enhance the rate of healing or replace tissues or organs. Soft biomaterials fabricated for muscle, ligament, tendon, articular cartilage tissue engineering exhibit valuable properties, such as biocompatibility, biodegradability, flexible, renewability, and cheaper. The combination of the polysaccharides and proteins with synthetic biodegradable polymers improved the mechanical strength and when added with inorganic calcium compounds could be exploited in hard tissue engineering, such as bone tissue engineering. The development of biomaterial for scaffolding in tissue engineering using polysaccharides and proteins could lead to the emergence of a new era of safer material to replace the damaged tissues in the body without suffering inflammation. Polysaccharides and proteins are consumed as food, and treating damaged tissue with food‐based biomaterial would not harm the humankind. In the near future, the development of natural polymer‐based biodegradable biomaterials by interdisciplinary and multidisciplinary research scientists is highly important.

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Enhanced mechanical performances and bioactivity of cell laden-graphene oxide/alginate hydrogels open new scenario for articular tissue engineering applications

Cited by 78 publications

References 52 publications

3D Bioprinting of Graphene Oxide-Incorporated Cell-laden Bone Mimicking Scaffolds for Promoting Scaffold Fidelity, Osteogenic Differentiation and Mineralization

3D Bioprinting of Graphene Oxide-Incorporated Cell-laden Bone Mimicking Scaffolds for Promoting Scaffold Fidelity, Osteogenic Differentiation and Mineralization

Synthesis and Physicochemical Characterization of Multiwalled Carbon Nanotubes/Hydroxamic Alginate Nanocomposite Scaffolds

Biodegradable Polymers for Biomedical and Tissue Engineering

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