Enhanced mitochondrial biogenesis is associated with the ameliorative action of creatine supplementation in rat soleus and cardiac muscles

Abstract

The current study focused on the effect of creatine supplementation with/without exercise on the expression of genes controlling mitochondrial biogenesis in skeletal and cardiac muscles, as well as its safety profile on the liver and kidney. A total of 40 male Wister rats were included in the present study. Two unexercised groups: The control sedentary group and the sedentary creatine-treated group (n=10) were treated daily with oral creatine (0.5 g/kg per day). Two exercised groups performed swimming exercise training 5 days/week for a period of 5 weeks; The Exercise training group, and exercise training and creatine (0.5 g/kg per day) treated group. After sacrifice, blood samples, cardiac and soleus muscles were collected for assessment of mtDNA copy number, gene expression analysis and nuclear extraction for the assay of PGC-1α. The results of the current study demonstrated that, physical activity with short-term creatine supplementation increased all factors of mitochondrial biogenesis, an effect that is devoid of any kidney or liver adverse effects. Further studies are still required to explore the potential of creatine supplementation in ameliorating mitochondrial diseases, including epilepsy, skeletal and cardiac myopathies, hepatopathies and nephropathies.

The pre-conception maternal exposure to Sofosbuvir affects the mitochondrial biogenesis in prenatal fetal tissues: Experimental study on rats

“…A specific primer pair for mtDNA and a primer pair for the nuclear PGC-1α gene (Table 1 ) were used. PCR reactions were carried out using SYBR Green PCR Master Mix (Qiagen, Germany), 0.5 μM of each primer pair, and 50 ng genomic DNA under the following conditions: 95˚C for 10 min followed by 40 cycles of 95 °C for 15 s, 60 °C for 30 s and 72 °C for 30 s (Gowayed et al 2020 ).…”

The Anti-Obesity Potential of Superparamagnetic Iron Oxide Nanoparticles against High-Fat Diet-Induced Obesity in Rats: Possible Involvement of Mitochondrial Biogenesis in the Adipose Tissues

“…A specific primer pair for mtDNA (forward: 5’-ACACCAAAAGGACGAACCTG-3’; reverse: 5’-ATGGGGAAGAAGCCCTAGAA-3’) and a primer pair for the nuclear PGC-1α gene (forward: 5’-ATGAATGCAGCGGTCTTAGC-3’; reverse: 5’-AACAATGGCAGGGTTTGTTC-3’) were used. PCR reactions were carried out using Rotor Gene SYBR Green PCR Kit (Qiagen ® , Germantown, MA, USA), 0.5 µM forward and reverse primer, and 50 ng of extracted DNA under the following conditions: 95 °C for 10 min followed by 40 cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s [ 26 ].…”

The Role of Mitochondria in Phytochemically Mediated Disease Amelioration

“…21 Lastly, it has been previously reported that exercise may lead to an increment in mitochondrial biogenesis because the free radicals generated during exercise become signals for PGC-1α to promote mitochondrial biogenesis. 22 In summary, PGC-1α and PPARs could promote mitochondrial biogenesis, resulting in increased mtDNA.…”

“…Besides, it has been reviewed that PPAR-α and PPAR-γ could also get involved, possibly by activating those transcription factors to promote mitochondria biogenesis . Lastly, it has been previously reported that exercise may lead to an increment in mitochondrial biogenesis because the free radicals generated during exercise become signals for PGC-1α to promote mitochondrial biogenesis . In summary, PGC-1α and PPARs could promote mitochondrial biogenesis, resulting in increased mtDNA.…”