Enhanced multi-phosphopeptide enrichment and Nano LC-ESI-qTOF-MS detection strategy using click OEG-CD matrix

Zhao, Yan‐Xia; Wang, Liheng; Guo, Zhimou; Chi, Xiao-Fei; Ma, Xiaochi; Qi, Yan; Shinong, Fang; Li, Xiuling; Liang, Xinmiao

doi:10.1007/s40242-014-4179-7

Cited by 2 publications

(1 citation statement)

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“…8 However, the determination of phosphopeptides in complex biological samples has remained a significant challenge because their scarcity in the total proteome as well as their deficient ionization and ion suppression during the mass spectrometric analysis. [9][10][11][12][13] Furthermore, the analysis of multi-phosphorylated peptides is particularly more difficult than that of mono-phosphorylated peptides due to their even lower abundance and severe ion suppression in MS. 14,15 Therefore, the development of strategies for highly selective phosphopeptide enrichment is in great demand. 16 Chelation/coordination chemistry is a most popular strategy among a number of approaches that have been developed for phosphopeptide enrichments.…”

Section: Introductionmentioning

confidence: 99%

Zinc Ion-immobilized Magnetic Microspheres for Enrichment and Identification of Multi-phosphorylated Peptides by Mass Spectrometry

et al. 2017

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The selective isolation of phosphorylated peptides and subsequent analysis using mass spectrometry is important for understanding how protein kinase and phosphatase signals can precisely modulate the on/off states of signal transduction pathways. However, the isolation and detection of multi-phosphorylated peptides is still limited due to their distinct affinity to various materials and their poor ionization efficiency. Here, we report a highly efficient and selective enrichment of phosphorylated peptides using binuclear Zn 2+ -dipicolylamine complex-coated magnetic microspheres (ZnMMs). ZnMMs can utilize the rapid and selective isolation/enrichment of phosphorylated peptides and the subsequent mass spectrometric analysis, given the intrinsic magnetic property of magnetic microspheres and the highly selective binding ability of the binuclear Zn 2+ -dipicolylamine complex to phosphate groups. α-Casein and β-casein were chosen for a proof-of-concept demonstration. We contemplated that phosphopeptides were selectively isolated and enriched from both the tryptic digests of casein proteins and mixed samples with a high degree of sensitivity by facilitating ZnMMs. Especially, ZnMMs showed high efficiency with multi-phosphopeptides, which are in general difficult to be examined by mass analysis on account of their poor ionization efficiency. For the model protein α, β-casein mixture of the tryptic digest, 17 phosphopeptides were identified with ZnMMs and 82% of the enriched phosphopeptides were multiphosphorylated peptides, indicating that ZnMMs have excellent enrichment efficiency and strong affinity towards multiphosphorylated peptides.

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Section: Introductionmentioning

confidence: 99%

Zinc Ion-immobilized Magnetic Microspheres for Enrichment and Identification of Multi-phosphorylated Peptides by Mass Spectrometry

et al. 2017

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Microwave-assisted one-pot synthesis of Ag NPs/C and its application in H2O2 and glucose detection

Sun

et al. 2016

Chem. Res. Chin. Univ.

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The facile preparation of Ag NPs/C via a one-pot strategy was carried out by microwave treatment of a mixed aqueous solution of AgNO 3 and glucose at 180 °C for 20 min without the presence of extra reducing agent. The as-synthesized Ag NPs/C showed high catalytic performance toward the reduction of H 2 O 2 . The H 2 O 2 sensor constructed with as-synthesized Ag NPs/C exhibited a short amperometric response time of less than 2 s. The linear range was approximately (0.1-50) mmol/L(r=0.997), and the detection limit was approximately 3.3 μmol/L at a signal-to-noise ratio of 3. A glucose biosensor was fabricated by immobilizing glucose oxidase onto Ag NPs/Cmodified glassy carbon electrode to detect glucose. The glucose sensor had a wide linear response range of 2-22 mmol/L(r=0.999) and a detection limit of 190 μmol/L.

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Enhanced multi-phosphopeptide enrichment and Nano LC-ESI-qTOF-MS detection strategy using click OEG-CD matrix

Cited by 2 publications

References 20 publications

Zinc Ion-immobilized Magnetic Microspheres for Enrichment and Identification of Multi-phosphorylated Peptides by Mass Spectrometry

Zinc Ion-immobilized Magnetic Microspheres for Enrichment and Identification of Multi-phosphorylated Peptides by Mass Spectrometry

Microwave-assisted one-pot synthesis of Ag NPs/C and its application in H2O2 and glucose detection

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