Enhanced nasal drug delivery efficiency by increasing mechanical loading using hypergravity

Kim, Dongjoo; Kim, Young Hyo; Kwon, Soonjo

doi:10.1038/s41598-017-18561-x

Cited by 35 publications

(22 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Actually in vitro studies showed that hypergravity may be an effective new strategy for that. Such a finding might be of great interest in space pharmacology ( Vernikos, in press ) where enhanced nasal drug delivery in microgravity may be desired ( Kim et al, 2018 ).…”

Section: Pharmaco-kinetics and –Dynamicsmentioning

confidence: 99%

Recent Progress in Space Physiology and Aging

Strollo¹,

Gentile²,

Strollo

et al. 2018

Front. Physiol.

View full text Add to dashboard Cite

Astronauts coming back from long-term space missions present with different health problems potentially affecting mission performance, involving all functional systems and organs and closely resembling those found in the elderly. This review points out the most recent advances in the literature in areas of expertise in which specific research groups were particularly creative, and as they relate to aging and to possible benefits on Earth for disabled people. The update of new findings and approaches in space research refers especially to neuro-immuno-endocrine-metabolic interactions, optic nerve edema, motion sickness and muscle-tendon-bone interplay and aims at providing the curious - and even possibly naïve young researchers – with a source of inspiration and of creative ideas for translational research.

show abstract

Section: Pharmaco-kinetics and –Dynamicsmentioning

confidence: 99%

Recent Progress in Space Physiology and Aging

Strollo¹,

Gentile²,

Strollo

et al. 2018

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…That influences the pharmacokinetics of systemically of locally delivered drugs. Increased cellular uptake of therapeutics on skeletal muscle cells or nasal epithelial cells has been reported, when the strain was applied [220,221]. This situation could also apply to bone cells, highlighting the importance of drug screening during applied mechanical loading.…”

Section: Drug Screening In the Presence Of Mechanical Loading Is Critmentioning

confidence: 97%

Mechanisms of mechanically induced Osteoclastogenesis : in a novel in vitro model for bone implant loosening

Bratengeier

2019

Linköping University Medical Dissertations

View full text Add to dashboard Cite

Total joint arthroplasty is the primary intervention in the treatment of end-stage osteoarthritis.The majority of joint arthroplasties will last the whole lifetime. However, in some patients, the replacement will fail during their lifetime requiring a revision of the implant. These revisions are strenuous for the patient and costly for health care. To avoid a revision, therefore, is the uttermost goal of modern orthopedics. Joint replacement at a younger age, in combination with a more active lifestyle, increases the need for an early revision of the joint prosthesis. The main reason for revision surgeries is aseptic loosening, a condition where the prosthesis is loosening due to bone degradation at the peri-prosthetic interface in the absence of infections. The most well-established pathological mechanism for aseptic loosening is related to wear particles, generated from different parts of the prosthesis that will trigger bone degradation and bone loss.In addition, early micromotions of the prosthesis and resulting local pressurized fluid flow in the peri-prosthetic interface (supraphysiological loading) have also been identified as a cause for aseptic loosening. However, it remains unknown what cells are the primary responders to supraphysiological loading, and what underlying physical, cellular and molecular mechanism that triggers osteoclast differentiation and osteolysis.The main goal of this thesis is to shed light on three currently unknown aspects of mechanical loading-induced peri-prosthetic osteolysis, leading to aseptic loosening of orthopedic prostheses: (1)Which cells are the primary responder to supraphysiological loading? (2)What characteristics of the mechanical stimulus induce an osteo-protective or osteo-destructive response? (3)Which cellular mechano-sensing mechanisms are involved in an osteo-destructive response?The first study focused on the creation and validation of a novel in vitro model for mechanical loading-induced bone degradation. This model simulates the mechanical (over)loading conditions around a loosening prosthesis, as observed in the clinics, and in animal models for mechanical induced osteolysis. Using the MLO-Y4 osteocyte-like cell line, we verified that osteocytes exposed to supraphysiological mechanical loading in our model induce differentiation of progenitor cells into osteoclasts by direct cell-to-cell communication and through the release of soluble factors. This was accompanied by the release of an increased concentration of nitric oxide and higher expression of membrane-bound RANKL, while the concentration of soluble RANKL was not altered. These findings confirm the successful translation of mechanical-induced bone loss to an in vitro model for bone implant loosening. Many people have supported me during my time as a Ph.D. student and I am deeply grateful. I want to highlight a few people specifically, as they contributed significantly to my success: Anna Fahlgren Thank you for giving me the opportunity to become part of your research team and guiding me to become a c...

show abstract

“…It appeared that the LCC/ALI switch was feasible 24 h after inoculation when starting from a seeding density of 4-5 × 10 5 cells/cm 2 (Pozzoli et al, 2014). Cell differentiation and the presence of tight junctions were effective after two weeks under ALI conditions, with TEER values of 50 and 120 Ω.cm 2 after 1 and 2 weeks, respectively (Kim et al, 2018;Pozzoli et al, 2016a,b).…”

Section: In Vitro Modelsmentioning

confidence: 99%

“…The model was first used for metabolic studies (e.g. degradation tests) and, subsequently, for transport experiments after culture optimization (Gonçalves et al, 2016;Kim et al, 2018;Shin et al, 2004). In fact, RPMI 2650 cells were subject to strong histological modifications when switching from LCC to ALI (e.g.…”

Section: In Vitro Modelsmentioning

confidence: 99%

How to characterize a nasal product. The state of the art of in vitro and ex vivo specific methods

Salade

Wauthoz

Goole

et al. 2019

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Nasal delivery offers many benefits over other conventional routes of delivery (e.g. oral or intravenous administration). Benefits include, among others, a fast onset of action, non-invasiveness and direct access to the central nervous system. The nasal cavity is not only limited to local application (e.g. rhinosinusitis) but can also provide direct access to other sites in the body (e.g. the central nervous system or systemic circulation). However, both the anatomy and the physiology of the nose impose their own limitations, such as a small volume for delivery or rapid mucociliary clearance. To meet nasal-specific criteria, the formulator has to complete a plethora of tests, in vitro and ex vivo, to assess the efficacy and tolerance of a new drug-delivery system. Moreover, depending on the desired therapeutic effect, the delivery of the drug should target a specific pathway that could potentially be achieved through a modified release of this drug. Therefore, this review focuses on specific techniques that should be performed when a nasal formulation is developed. The review covers both the tests recommended by regulatory agencies (e.g. the Food and Drug Administration) and other complementary experiments frequently performed in the field.

show abstract

Enhanced nasal drug delivery efficiency by increasing mechanical loading using hypergravity

Cited by 35 publications

References 43 publications

Recent Progress in Space Physiology and Aging

Recent Progress in Space Physiology and Aging

Mechanisms of mechanically induced Osteoclastogenesis : in a novel in vitro model for bone implant loosening

How to characterize a nasal product. The state of the art of in vitro and ex vivo specific methods

Contact Info

Product

Resources

About