Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro

Shi, Wentao; Bian, Lu; Lv, Demin; Bi, Sheng; Dai, Yao; Yang, Kaiyuan; Lu, Hao; Zhou, Huangao; Que, Yunduan; Wang, Dongming; Zhang, Zhijian; Lu, Naiyan

doi:10.1007/s00726-020-02918-0

Cited by 15 publications

(10 citation statements)

References 31 publications

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“…The rats were deeply anesthetized by an intraperitoneal injection of phenobarbital sodium (200 mg/kg). The isolation of EMSCs was constructed based on the protocol described in our previous study [ 17 ]. Briefly, respiratory mucosa tissue samples from the lower two-thirds of the nasal septum were carefully isolated and completely sheared to pieces.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Efficiency of Nasal Mucosa-Derived Ectodermal Mesenchymal Stem Cells in Rats with Acute Hepatic Failure

Xiang

Wang

et al. 2023

Stem Cells International

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Background. Liver transplantation is limited by the insufficiency of liver organ donors when treating end-stage liver disease or acute liver failure (ALF). Ectodermal mesenchymal stem cells (EMSCs) derived from nasal mucosa have emerged as an alternative cell-based therapy. However, the role of EMSCs in acute liver failure remains unclear. Methods. EMSCs were obtained from the nasal mucosa tissue of rats. First, EMSCs were seeded on the gelatin-chitosan scaffolds, and the biocompatibility was evaluated. Next, the protective effects of EMSCs were investigated in carbon tetrachloride- (CCl4-) induced ALF rats. Finally, we applied an indirect coculture system to analyze the paracrine effects of EMSCs on damaged hepatocytes. A three-step nontransgenic technique was performed to transform EMSCs into hepatocyte-like cells (HLCs) in vitro. Results. EMSCs exhibited a similar phenotype to other mesenchymal stem cells along with self-renewal and multilineage differentiation capabilities. EMSC-seeded gelatin-chitosan scaffolds can increase survival rates and ameliorate liver function and pathology of ALF rat models. Moreover, transplanted EMSCs can secrete paracrine factors to promote hepatocyte regeneration, targeted migration, and transdifferentiate into HLCs in response to the liver’s microenvironment, which will then repair or replace the damaged hepatocytes. Similar to mature hepatocytes, HLCs generated from EMSCs possess functions of expressing specific hepatic markers, storing glycogen, and producing urea. Conclusions. These results confirmed the feasibility of EMSCs in acute hepatic failure treatment. To our knowledge, this is the first time that EMSCs are used in the therapy of liver diseases. EMSCs are expected to be a novel and promising cell source in liver tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Therapeutic Efficiency of Nasal Mucosa-Derived Ectodermal Mesenchymal Stem Cells in Rats with Acute Hepatic Failure

Xiang

Wang

et al. 2023

Stem Cells International

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“…These results agreed with our previous studies. [21,22] EMSCs are a multipotent progenitor population derived from the cranial neural crest that can be isolated from the oral mucosa and olfactory and respiratory mucosa. [23] These cells present multipotentiality, giving rise to the majority of the bone and cartilage of the head and face.…”

Section: Identification Of Emscsmentioning

confidence: 99%

Enhanced Bone Regeneration Using a ZIF‐8‐Loaded Fibrin Composite Scaffold

Shi

Bian

et al. 2022

Macromolecular Bioscience

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In the present study, fibrin‐based biomaterials made of zeolite imidazole framework‐8 (ZIF‐8) and fibrin gel (Z‐FG) are fabricated with the aim of enhancing skull regeneration. X‐ray diffraction (XRD), scanning electron microscopy (SEM), ultraviolet (UV)–vis spectrophotometry, Fourier transform infrared spectroscopy, and rheometry are used to characterize ZIF‐8 and Z‐FG. The influences of ZIF‐8 on the physical properties of fibrin gel (e.g., porosity, modulus, and in vitro biodegradation) are investigated, and the effect of ZIF‐8 concentration on fibrin gel properties in vitro is determined by seeding ectomesenchymal stem cells (EMSCs) over Z‐FG. EMSC osteogenic differentiation reveals higher expression of bone‐related proteins and higher calcium deposition and alkaline phosphatase activity, indicating that Z‐FG may be a good osteoinductive biomaterial. Furthermore, these results show that the piezochannel and yes‐associated protein (YAP) signaling pathway are involved in the differentiation process. In addition, the in vivo results demonstrate that Z‐FG increases bone formation in critical‐sized calvarial defects in rats. Thus, the developed composite scaffold may be a suitable biomaterial for skull tissue‐engineering applications.

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“…Zhijian Zhang et al isolated EMSCs from nasal olfactory and respiratory mucosa and proved their preference for neural directional differentiation in threedimensional scaffolds [11]. Moreover, EMSCs, similar to other MSCs, possess great paracrine effects that determine the fate of other cells such as oligodendrocyte precursor cells [12] and NSCs [13]. Strikingly, Wenwen Deng et al illustrated that the enhanced neuronal differentiation and inhibited astrocytic differentiation of NSCs when co-culturing with EMSCs is due to the assembly of a cell-cell contact all-inone lipid raft niche via the connexin43/YAP/143-3 pathway [14].…”

Section: Introductionmentioning

confidence: 99%

Enhanced neuronic differentiation of neural stem cells by SHH gene-engineered EMSCs co-culture in vitro

Sun

Liu

et al. 2023

Preprint

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Spinal cord injury is a severe central nervous system disease. Neural crest-derived ectoderm mesenchymal stem cells (EMSCs) have become an important tool in treating SCI owing to their excellent neuronal differentiation efficiency and promising paracrine effects. Sonic hedgehog (shh) was proven to promote both the proliferation and chemotaxis of NSCs and induce their differentiation into mature neurons and the formation of synapses. In this study, we examined the neuronal differentiation induced by SHH-engineered EMSCs in a co-culture system. The levels of the neuronal markers, tubulin beta 3 class III and growth-associated protein 43, were higher in NSCs in the SHH-EMSC co-culture group, and those of the astrocytic marker glial fibrillary acidic protein (GFAP) were lower, compared with the mono and EMSCs-NSCs groups The expression of GAP43, GFAP, MBP, and NF200 was also confirmed by western blot analysis. Most importantly, the upregulation of con43, con47, smoothen, and integrin might reveal the construction of the gap junction during the overexpression of SHH in EMSCs. Overall, our findings indicate that SHH-EMSCs might facilitate the neuronal differentiation of NSCs via the gap junction formation.

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Enhanced neural differentiation of neural stem cells by sustained release of Shh from TG2 gene-modified EMSC co-culture in vitro

Cited by 15 publications

References 31 publications

Therapeutic Efficiency of Nasal Mucosa-Derived Ectodermal Mesenchymal Stem Cells in Rats with Acute Hepatic Failure

Therapeutic Efficiency of Nasal Mucosa-Derived Ectodermal Mesenchymal Stem Cells in Rats with Acute Hepatic Failure

Enhanced Bone Regeneration Using a ZIF‐8‐Loaded Fibrin Composite Scaffold

Enhanced neuronic differentiation of neural stem cells by SHH gene-engineered EMSCs co-culture in vitro

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