Enhanced neuronal activity in mouse motor cortex with microbubbles’ oscillations by transcranial focused ultrasound stimulation

Cui, Zhiwei; Li, Dapeng; Feng, Yang; Xu, Tianqi; Wu, Shan; Li, Yibao; Bouakaz, Ayache; Wan, Mingxi; Zhang, Siyuan

doi:10.1016/j.ultsonch.2019.104745

Cited by 28 publications

(11 citation statements)

References 59 publications

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“…SonoVue microbubbles, whose mean radius is about 2.5 μm [28] , were purchased from Bracco (Bracco diagnostics Inc., Geneva, Switzerland). Before the experiments, the vial of SonoVue would be vented with a sterile needle (18-gauge), followed by the injection of PBS to make SonoVue microbubble solutions.…”

Section: Methodsmentioning

confidence: 99%

Cavitation-facilitated transmembrane permeability enhancement induced by acoustically vaporized nanodroplets

Song

Zhang

Teng

et al. 2021

Ultrasonics Sonochemistry

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Highlights Mechanisms of membrane permeability enhanced by vaporized nanodroplets. Cavitation induced by droplets compared with miicrobubbles at varied concentrations. Membrane permeability enhanced by cavitation droplets and bubbles compared in vitro . In vitro evaluation of the correlation between IC dose and membrane permeability. BBB opening induced by vaporized nanodroplets and microbubbles compared in vivo .

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Section: Methodsmentioning

confidence: 99%

Cavitation-facilitated transmembrane permeability enhancement induced by acoustically vaporized nanodroplets

Song

Zhang

Teng

et al. 2021

Ultrasonics Sonochemistry

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“…That study clearly demonstrated a neuromodulatory effect of FUS-BBBO, and the underlying mechanism may have had both vascular and neuronal origins [ 106 ]. Cui et al demonstrated that reducing the ultrasound energy in the presence of microbubbles so as to not induce BBBO could still produce neuronal excitation [ 112 ]. This observation indicates that neural stimulation can originate from ultrasound-induced amplification of the radiation force and microstreaming of microbubbles and might not require the occurrence of BBBO.…”

Section: Other Cns Applicationsmentioning

confidence: 99%

Focused Ultrasound Combined with Microbubbles in Central Nervous System Applications

2021

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The blood–brain barrier (BBB) protects the central nervous system (CNS) from invasive pathogens and maintains the homeostasis of the brain. Penetrating the BBB has been a major challenge in the delivery of therapeutic agents for treating CNS diseases. Through a physical acoustic cavitation effect, focused ultrasound (FUS) combined with microbubbles achieves the local detachment of tight junctions of capillary endothelial cells without inducing neuronal damage. The bioavailability of therapeutic agents is increased only in the area targeted by FUS energy. FUS with circulating microbubbles is currently the only method for inducing precise, transient, reversible, and noninvasive BBB opening (BBBO). Over the past decade, FUS-induced BBBO (FUS-BBBO) has been preclinically confirmed to not only enhance the penetration of therapeutic agents in the CNS, but also modulate focal immunity and neuronal activity. Several recent clinical human trials have demonstrated both the feasibility and potential advantages of using FUS-BBBO in diseased patients. The promising results support adding FUS-BBBO as a multimodal therapeutic strategy in modern CNS disease management. This review article explores this technology by describing its physical mechanisms and the preclinical findings, including biological effects, therapeutic concepts, and translational design of human medical devices, and summarizes completed and ongoing clinical trials.

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“…Seven studies looked at stimulation of the M1 in healthy mice and found that LI-TUS induced changes in the activity of M1 that correlated to the success rate of the motor response (Kim et al, 2019b;Wang et al, 2019d) in electromyography (EMG), local field potential (LFP) (Wang et al, 2019c,d;Yuan et al, 2020), and cortical blood flow (Yuan et al, 2020). Specifically, EMG signals from the triceps muscles of forelimbs (Cui et al, 2019(Cui et al, , 2020, the right distal forelimb (Wang et al, 2019a), bilateral whiskers, and tail (Wang et al, 2019d;Yuan et al, 2020) were examined for motor response changes in M1 after LI-TUS. EMG response latency was significantly decreased with higher acoustic pressure of 0.12 and 0.25 MPa (Cui et al, 2019(Cui et al, , 2020 while EMG amplitudes were increased with higher LI-TUS intensity (Wang et al, 2019a;Yuan et al, 2020).…”

Section: Neuromodulatory Effect Of Tus In Animalsmentioning

confidence: 99%

“…Specifically, EMG signals from the triceps muscles of forelimbs (Cui et al, 2019(Cui et al, , 2020, the right distal forelimb (Wang et al, 2019a), bilateral whiskers, and tail (Wang et al, 2019d;Yuan et al, 2020) were examined for motor response changes in M1 after LI-TUS. EMG response latency was significantly decreased with higher acoustic pressure of 0.12 and 0.25 MPa (Cui et al, 2019(Cui et al, , 2020 while EMG amplitudes were increased with higher LI-TUS intensity (Wang et al, 2019a;Yuan et al, 2020). LI-TUS with intensity at 1.1 W/cm 2 (I SPPA ) over M1 was able to elicit the greatest motor response in bilateral whiskers and tail and resulted in an increase in the peak values of cortical blood flow and neural activity responses (Yuan et al, 2020).…”

Section: Neuromodulatory Effect Of Tus In Animalsmentioning

confidence: 99%

“…Different intensity levels were used including 0.20, 0.40, 0.48, 0.53, 0.80, and from 1.10 to 2.11 W/cm 2 ( I SPPA) and frequencies in the range of 0.18-0.62 MHz. LI-TUS is capable of eliciting a motor response (Cui et al, 2019(Cui et al, , 2020Kim et al, 2019b;Wang et al, 2019a,c,d;Yuan et al, 2020) and inducing cortico-muscular synchronization at specific frequency bands of 5-150 Hz (Wang et al, 2019d). For example, Wang et al (2019d) showed that alpha and beta synchronization appeared at 0.20 and 0.40 W/cm 2 (I SPPA ) while gamma synchronization occurred at 0.80 and 1.1 W/cm 2 ( I SPPA) stimulation.…”

Section: Neuromodulatory Effect Of Tus In Animalsmentioning

confidence: 99%

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Effect of Low Intensity Transcranial Ultrasound Stimulation on Neuromodulation in Animals and Humans: An Updated Systematic Review

et al. 2021

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Background: Although low-intensity transcranial ultrasound stimulation (LI-TUS) has received more recognition for its neuromodulation potential, there remains a crucial knowledge gap regarding the neuromodulatory effects of LI-TUS and its potential for translation as a therapeutic tool in humans.Objective: In this review, we summarized the findings reported by recently published studies regarding the effect of LI-TUS on neuromodulation in both animals and humans. We also aim to identify challenges and opportunities for the translation process.Methods: A literature search of PubMed, Medline, EMBASE, and Web of Science was performed from January 2019 to June 2020 with the following keywords and Boolean operators: [transcranial ultrasound OR transcranial focused ultrasound OR ultrasound stimulation] AND [neuromodulation]. The methodological quality of the animal studies was assessed by the SYRCLE's risk of bias tool, and the quality of human studies was evaluated by the PEDro score and the NIH quality assessment tool.Results: After applying the inclusion and exclusion criteria, a total of 26 manuscripts (24 animal studies and two human studies) out of 508 reports were included in this systematic review. Although both inhibitory (10 studies) and excitatory (16 studies) effects of LI-TUS were observed in animal studies, only inhibitory effects have been reported in primates (five studies) and human subjects (two studies). The ultrasonic parameters used in animal and human studies are different. The SYRCLE quality score ranged from 25 to 43%, with a majority of the low scores related to performance and detection bias. The two human studies received high PEDro scores (9/10).Conclusion: LI-TUS appears to be capable of targeting both superficial and deep cerebral structures to modulate cognitive or motor behavior in both animals and humans. Further human studies are needed to more precisely define the effective modulation parameters and thereby translate this brain modulatory tool into the clinic.

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Enhanced neuronal activity in mouse motor cortex with microbubbles’ oscillations by transcranial focused ultrasound stimulation

Cited by 28 publications

References 59 publications

Cavitation-facilitated transmembrane permeability enhancement induced by acoustically vaporized nanodroplets

Cavitation-facilitated transmembrane permeability enhancement induced by acoustically vaporized nanodroplets

Focused Ultrasound Combined with Microbubbles in Central Nervous System Applications

Effect of Low Intensity Transcranial Ultrasound Stimulation on Neuromodulation in Animals and Humans: An Updated Systematic Review

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