Enhanced nonenzymatic glycosylation of blood proteins in stored blood

Szelényi, Judith; Földi, J.; Hollán, Susan R.

doi:10.1046/j.1537-2995.1983.23183147296.x

Cited by 11 publications

(13 citation statements)

References 23 publications

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“…A slowdown in glycerate excretion after Day 14 was clearly observed. Interestingly, differences in glycated Hb are observed only after 14 days of storage . In light of our results we propose that glycerate excretion is a measure of protein deglycosylation activity during storage.…”

Section: Discussionsupporting

confidence: 56%

“…Interestingly, differences in glycated Hb are observed only after 14 days of storage. 56,57 In light of our results we propose that glycerate excretion is a measure of protein deglycosylation activity during storage. After 2 weeks of storage deglycosylation activity slows down due to suppressed activity of fructosamine 3-kinase on account of lowered ATP levels.…”

Section: Discussionmentioning

confidence: 76%

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Mannose and fructose metabolism in red blood cells during cold storage in SAGM

et al. 2017

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BACKGROUND: Alternate sugar metabolism during red blood cell (RBC) storage is not well understood. Here we report fructose and mannose metabolism in RBCs during cold storage in SAGM and the impact that these monosaccharides have on metabolic biomarkers of RBC storage lesion. STUDY DESIGN AND METHODS: RBCs were storedin SAGM containing uniformly labeled 13 C-fructose or 13 C-mannose at 9 or 18 mmol/L concentration for 25 days. RBCs and media were sampled at 14 time points during storage and analyzed using ultraperformance liquid chromatography-mass spectrometry. Blood banking quality assurance measurements were performed. RESULTS:Red blood cells incorporated fructose and mannose during cold storage in the presence of glucose. Mannose was metabolized in preference to glucose via glycolysis. Fructose lowered adenosine triphosphate (ATP) levels and contributed little to ATP maintenance when added to SAGM. Both monosaccharides form the advanced glycation end product glycerate. Mannose activates enzymes in the RBC that take part in glycan synthesis. CONCLUSIONS:Fructose or mannose addition to RBC SAGM concentrates may not offset the shift in metabolism of RBCs that occurs after 10 days of storage. Fructose and mannose metabolism at 48C in SAGM reflects their metabolism at physiologic temperature. Glycerate excretion is a measure of protein deglycosylation activity in stored RBCs. No cytoprotective effect was observed upon the addition of either fructose or mannose to SAGM.R ed blood cells (RBCs) are routinely stored for up to 42 days until required as support during surgery and/or after trauma-related injury. 1 The quality of RBCs deteriorates during storage through a process known as RBC storage lesion (RSL) that ultimately compromises the RBC's functionality in oxygen transport.2 RSL is a complex biochemical process that negatively impacts the structure and function of diverse biochemical components. 3,4 Finding ways to counteract RSL has gained attention after findings indicating a correlation of RBC unit age to adverse posttransfusion clinical outcomes. [5][6][7][8] Although these links have not been found in large clinical trials, 9 RSL represents a model to explore RBC biology and advance cell preservation technology. One way to slow down RSL is through the manipulation of RBC metabolism. During cold storage, RBC metabolism can be described by three time-dependent and distinct metabolic phenotypes that have recently been established in additive solutions (ASs) used to store MATERIALS AND METHODS RBC collection and samplingA quantity of 460 mL of whole blood was obtained from five healthy donors of mixed sex and blood type. RBC preparation and storage were as previously described 8 with the following modifications: One day before RBC donation, four carbohydrate stock solutions were prepared by dissolving either 13 C uniformly labeled fructose (1.689 or 3.379 g) or mannose (1.689 or 3.379 g; Cambridge Isotope Laboratories, Inc.) in four separate tubes containing a final volume of 5 mL of SAGM. These four stock so...

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 76%

Mannose and fructose metabolism in red blood cells during cold storage in SAGM

et al. 2017

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“…Blood for use in transfusions is stored in a medium containing d ‐glucose at 35 m m for up to 35 days at 4°. Storage of blood in this manner has been shown previously to increase the proportion of glycated haemoglobin 30 and it is therefore likely that CD59 on stored E is to some degree glycated and inactivated, even with storage at low temperature. Transfused E may then be more susceptible to C lysis and display a shortened lifespan in vivo .…”

Section: Discussionmentioning

confidence: 94%

Glycation of CD59 impairs complement regulation on erythrocytes from diabetic subjects

2005

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SummaryType 1 diabetes is associated with anaemia. Although the underlying mechanisms remain unclear, the accompanying reticulocytosis implies that erythrocyte lifespan in the circulation is shortened. Among the factors that permit prolonged survival of erythrocytes are the membrane complement regulators. In conditions such as paroxysmal nocturnal haemoglobinuria, where erythrocyte expression of these regulators is reduced, erythrocyte survival is compromised and anaemia follows. Recent in vitro evidence indicates that one of the key membrane complement regulators, CD59, is inactivated by glycation in the presence of high concentrations of glucose or other glycating sugars. To ascertain whether glycationinduced inactivation of CD59 occurrs in vivo we examined CD59 surface expression and function on erythrocytes from a cohort with poorly controlled type 1 diabetes (hyperglycaemic) and from matched normoglycaemic controls. Although expression of CD59, assessed using polyclonal anti-CD59 antiserum, was similar in the two groups, erythrocytes from hyperglycaemic individuals were more susceptible to lysis by complement, entirely as a result of the loss of functional CD59. These data implicate glycation-induced inactivation of CD59 as a factor contributing to anaemia in type 1 diabetes.

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“…Haemoglobin A 1c (HbA 1c ) represents a key biomarker in diabetes diagnosis and management, since it allows clinicians to estimate mean blood glucose concentration in the recent period preceding withdrawal . Indeed, glycation of haemoglobin is a non‐enzymatic irreversible process that is promoted by the prolonged exposure of erythrocytes to high glucose concentrations , a condition that is known to occur under blood banking conditions, where additive solutions (such as SAGM) expose red blood cells (RBCs) to higher than normal glycemic levels . However, controversial data indicate no clear hint as to whether and to which extent HbA 1c accumulates during red blood cell storage .…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, glycation of haemoglobin is a non‐enzymatic irreversible process that is promoted by the prolonged exposure of erythrocytes to high glucose concentrations , a condition that is known to occur under blood banking conditions, where additive solutions (such as SAGM) expose red blood cells (RBCs) to higher than normal glycemic levels . However, controversial data indicate no clear hint as to whether and to which extent HbA 1c accumulates during red blood cell storage . Indeed, data from the older literature support the hypothesis that high glucose concentrations in RBC storage medium end up promoting glycation of Hb, thus resulting in the accumulation of HbA 1c over time .…”

Section: Introductionmentioning

confidence: 99%

Haemoglobin glycation (Hb1Ac) increases during red blood cell storage: a MALDI‐TOF mass‐spectrometry‐based investigation

2013

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Haemoglobin A1c (HbA1c ) represents a key biomarker in diabetes diagnosis and management, as it is indicative of recent blood glucose concentrations. Glycation of haemoglobin is a non-enzymatic irreversible process that is promoted by the prolonged exposure of erythrocytes to high glucose concentrations, a condition that is known to occur under blood banking conditions. However, controversial data indicate no clear hint as to whether and to which extent HbA1c accumulates during red blood cell storage. Hereby, we propose the application of a validated MALDI-TOF mass-spectrometry-based method to this issue and report the observation about HbA1c levels apparently increasing over storage progression.

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Enhanced nonenzymatic glycosylation of blood proteins in stored blood

Cited by 11 publications

References 23 publications

Mannose and fructose metabolism in red blood cells during cold storage in SAGM

Mannose and fructose metabolism in red blood cells during cold storage in SAGM

Glycation of CD59 impairs complement regulation on erythrocytes from diabetic subjects

Haemoglobin glycation (Hb1Ac) increases during red blood cell storage: a MALDI‐TOF mass‐spectrometry‐based investigation

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