Enhanced NRT1.1/NPF6.3 expression in shoots improves growth under nitrogen deficiency stress in Arabidopsis

Sakuraba, Yasuhito; Chaganzhana,; Mabuchi, Atsushi; Iba, Koh; Yanagisawa, Shuichi

doi:10.1038/s42003-021-01775-1

Cited by 29 publications

(27 citation statements)

References 72 publications

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“…The selected member of NRT/PTR family was commonly downregulated in all conditions. Transporters of NRT1/PTR family function as low-affinity transporters (Sakuraba et al, 2021) and show down-regulation in response to N deficiency in plants (Islam, 2019;Lejay et al, 1999) and P starvation in barley (Long et al, 2019). The transcript levels of these three genes obtained by qRT-PCR were consistent with the patterns apparent in the RNAseq analysis; PHT1.1 and PHT1.13 were upregulated in LP, while NRT/PTR family 6.1 showed significant reduction in transcript levels in LN and LNP (Supplemental Figure S1A).…”

Section: Rna Sequencing Data Analysis and Identification Of Different...mentioning

confidence: 99%

Nitrogen and phosphorus deficiencies alter primary and secondary metabolites of soybean roots

Nezamivand-Chegini

Metzger

Moghadam

et al. 2022

Preprint

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Nitrogen (N) and phosphorus (P) are two essential plant macronutrients that can limit plant growth by different mechanisms. We aimed to shed light on how soybean respond to low nitrogen (LN), low phosphorus (LP) and their combined deficiency (LNP). Generally, these conditions triggered changes in gene expression of the same processes, including cell wall organization, defense response, response to oxidative stress, and photosynthesis, however, response was different in each condition. A typical primary response to LN and LP was detected also in soybean, i.e., the enhanced uptake of N and P, respectively, by upregulation of genes for the corresponding transporters. The regulation of genes involved in cell wall organization showed that in LP roots tended to produce more casparian strip, in LN more secondary wall biosynthesis occurred, and in LNP reduction in expression of genes involved in secondary wall production accompanied by cell wall loosening was observed. Flavonoid biosynthesis also showed distinct pattern of regulation in different conditions: more anthocyanin production in LP, and more isoflavonoid production in LN and LNP, which we confirmed also on the metabolite level. Interestingly, in soybean the nutrient deficiencies reduced defense response by lowering expression of genes involved in defense response, suggesting a role of N and P nutrition in plant disease resistance. In conclusion, we provide detailed information on how LN, LP, and LNP affect different processes in soybean roots on the molecular and physiological levels.

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Section: Rna Sequencing Data Analysis and Identification Of Different...mentioning

confidence: 99%

Nitrogen and phosphorus deficiencies alter primary and secondary metabolites of soybean roots

Nezamivand-Chegini

Metzger

Moghadam

et al. 2022

Preprint

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“…It can be seen that MeNPF4.5 responds to NO 3 – in the short term like many other plants NRT1.1 ( Cárdenas Navarro et al, 1998 ; Okamoto et al, 2003 ; Wang et al, 2007 ). In addition to being expressed in roots, AtNRT1.1 is also expressed in shoots, young leaves, and developing flower buds of Arabidopsis , but it is more strongly expressed in roots ( Miller et al, 2007 ; Sakuraba et al, 2021 ). Consistent with the expression pattern of NRT1.1 in Arabidopsis , MeNPF4.5 is expressed in roots, stems, and leaves of cassava plants, and it is predominantly expressed in roots.…”

Section: Discussionmentioning

confidence: 99%

“…At present, NRT/NPF genes have mainly been identified in A. thaliana and rice, and their functions and regulatory mechanisms have been studied in detail ( Liu et al, 2015 ; Sakuraba et al, 2021 ). For example, studies have shown that rice NRT1.1B is located in the cell membrane and plays an important role in the transport of NO 3 – from the adventitious roots to the leaves ( Hu et al, 2015 ; Zhao et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

MeNPF4.5 Improves Cassava Nitrogen Use Efficiency and Yield by Regulating Nitrogen Uptake and Allocation

Liang

Dong

et al. 2022

Front. Plant Sci.

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Improving nitrogen use efficiency (NUE) is a very important goal of crop breeding throughout the world. Cassava is an important food and energy crop in tropical and subtropical regions, and it mainly use nitrate as an N source. To evaluate the effect of the nitrate transporter gene MeNPF4.5 on the uptake and utilization of N in cassava, two MeNPF4.5 overexpression lines (MeNPF4.5 OE-22 and MeNPF4.5 OE-34) and one MeNPF4.5 RNA interference (RNAi) line (MeNPF4.5 Ri-1) were used for a tissue culture experiment, combining with a field trial. The results indicated that MeNPF4.5 is a plasma membrane transporter mainly expressed in roots. The gene is induced by NO3–. Compared with the wild type, MeNPF4.5 OE-22 exhibited improved growth, yield, and NUE under both low N and normal N levels, especially in the normal N treatment. However, the growth and N uptake of RNAi plants were significantly reduced, indicating poor N uptake and utilization capacity. In addition, photosynthesis and the activities of N metabolism-related enzymes (glutamine synthetase, glutamine oxoglutarate aminotransferase, and glutamate dehydrogenase) of leaves in overexpression lines were significantly higher than those in wild type. Interestingly, the RNAi line increased enzymatic activity but decreased photosynthesis. IAA content of roots in overexpressed lines were lower than that in wild type under low N level, but higher than that of wild type under normal N level. The RNAi line increased IAA content of roots under both N levels. The IAA content of leaves in the overexpression lines was significantly higher than that of the wild type, but showed negative effects on that of the RNAi lines. Thus, our results demonstrated that the MeNPF4.5 nitrate transporter is involved in regulating the uptake and utilization of N in cassava, which leads to the increase of N metabolizing enzyme activity and photosynthesis, along with the change of endogenous hormones, thereby improving the NUE and yield of cassava. These findings shed light that MeNPF4.5 is involved in N use efficiency use in cassava.

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“…Among the NRT1/PTR family proteins, NRT1.1 is considered as a unique protein, since it acts as a dual affinity transporter that can facilitate nitrate uptake at concentrations ranging from micromolar to millimolar (Liu et al, 1999). Genome-wide association study (GWAS) of Arabidopsis accessions suggested a significant association between differences in N starvation-induced leaf yellowing and NRT1.1 sequence diversity (Sakuraba et al, 2021b). Indeed, nrt1.1 knockout mutant, chl1-5 exhibited accelerated leaf yellowing, while transgenic NRT1.1-overexpressing (NRT1.1-OX) plants retained greenness under N-deficient conditions (Sakuraba et al, 2021b).…”

Section: Roles Of Nitrate Transporters In N Remobilizationmentioning

confidence: 99%

“…In addition, leaf yellowing due to the remobilization of N sources from older leaves to younger leaves and reproductive organs is also one of the representative N deficiency responses and is considerably important for plants to sustain growth and productivity. On the other hand, N starvation-induced leaf yellowing in young seedlings causes severe growth defects (Sakuraba et al, 2021b). Therefore, understanding the molecular mechanisms underlying N starvation-induced leaf senescence is critical for establishing sustainable agriculture under N-deficient conditions.…”

Section: Introductionmentioning

confidence: 99%

Molecular basis of nitrogen starvation-induced leaf senescence

Sakuraba

2022

Front. Plant Sci.

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Nitrogen (N), a macronutrient, is often a limiting factor in plant growth, development, and productivity. To adapt to N-deficient environments, plants have developed elaborate N starvation responses. Under N-deficient conditions, older leaves exhibit yellowing, owing to the degradation of proteins and chlorophyll pigments in chloroplasts and subsequent N remobilization from older leaves to younger leaves and developing organs to sustain plant growth and productivity. In recent years, numerous studies have been conducted on N starvation-induced leaf senescence as one of the representative plant responses to N deficiency, revealing that leaf senescence induced by N deficiency is highly complex and intricately regulated at different levels, including transcriptional, post-transcriptional, post-translational and metabolic levels, by multiple genes and proteins. This review summarizes the current knowledge of the molecular mechanisms associated with N starvation-induced leaf senescence.

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Enhanced NRT1.1/NPF6.3 expression in shoots improves growth under nitrogen deficiency stress in Arabidopsis

Cited by 29 publications

References 72 publications

Nitrogen and phosphorus deficiencies alter primary and secondary metabolites of soybean roots

Nitrogen and phosphorus deficiencies alter primary and secondary metabolites of soybean roots

MeNPF4.5 Improves Cassava Nitrogen Use Efficiency and Yield by Regulating Nitrogen Uptake and Allocation

Molecular basis of nitrogen starvation-induced leaf senescence

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