Enhanced nuclear localization of YAP1‐2 contributes to EGF‐induced EMT in NSCLC

Guo, Qiang; Quan, Mei‐Yu; Xu, Lingxia; Cai, Yiqi; Cai, J.L.; Xue, Li; Feng, Guifeng; Chen, Aiping; Yang, Weiwei; Dhlamini, Qhaweni; Jiang, Tianfang; Shen, Chengguo; Chen, Chengshui; Zhang, Jin‐San

doi:10.1111/jcmm.17150

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…The nuclear-localized expression of YAP1 in poromas with the YAP1–MAML2 gene rearrangement has been confirmed by Sekine et al. [ 12 ] The Hippo pathway, which is closely related to EMT, is transcriptionally activated as evidenced by the nuclear-localized expression of YAP1 [ 14 , 15 ]. In three out of the five MT cases, immunohistochemistry revealed nuclear localized expression of YAP1, but this expression was not diffuse and was primarily nuclear positivity in spindle cells.…”

Section: Discussionmentioning

confidence: 88%

Significance of YAP1–MAML2 rearrangement and GTF2I mutation in the diagnosis and differential diagnosis of metaplastic thymoma

Wang

Han

et al. 2023

Annals of Medicine

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Background Metaplastic thymoma (MT) is a very uncommon thymoma type, with biphasic differentiation as one of its histological characteristics. This histological pattern, however, can also be mistaken for type A thymoma and the A component in type AB thymoma. Methods Postoperative specimens were collected from five MT and four type A thymomas with a retrospective analysis involving immunohistochemistry, fluorescence in situ hybridization (FISH) and next-generation sequencing (NGS). Results The histological morphology of the MT overlapped with that of the type A thymoma. With immunostains, the former’s spindle cell components expressed vimentin and EMA, but not CD20. In MT, 3/5 cases had the nuclear expression of YAP1. The spindle cell component of the type A thymoma was found to express CD20. In all five cases of MT, FISH detection revealed YAP1–MAML2 fusion, which was not found in any type A thymoma cases. NGS sequencing confirmed YAP1–MAML2 rearrangement in all five cases of MT, and mutations in POLE and HRAS were also found in two cases, respectively. GTF2I c.74146970 T > A mutations were found in all cases of type A thymoma, and HRAS and NRAS mutations were found in two cases, but no YAP1–MAML2 rearrangement was evident. Conclusions For the diagnosis and differential diagnosis of challenging cases, the YAP1–MAML2 rearrangement and GTF2I mutation were both significant molecular events specific to MT and type A thymoma, respectively.

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Section: Discussionmentioning

confidence: 88%

Significance of YAP1–MAML2 rearrangement and GTF2I mutation in the diagnosis and differential diagnosis of metaplastic thymoma

Wang

Han

et al. 2023

Annals of Medicine

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show abstract

“…Briefly, single cell suspensions were seeded in 96‐well plates at a density of 5000 cells/well and cultured for 0, 24, 48, and 72 h, respectively, before adding 10% of CCK‐8 solution and incubated for another 2 h. The absorbance values were measured at 450 nm by microplate reader (BioTek, USA). Scratch healing assays were performed as previously described 28 . Cells were cultured overnight to reach 90% confluency in a 6‐well plate before scratching with a 20‐μl plastic pipette tip.…”

Section: Methodsmentioning

confidence: 99%

“…Scratch healing assays were performed as previously described. 28 Cells were cultured overnight to reach 90% confluency in a 6‐well plate before scratching with a 20‐μl plastic pipette tip. The cells that migrated into the wounded areas were imaged with the Microscope (Leica, Germany).…”

Section: Methodsmentioning

confidence: 99%