Enhanced oral insulin delivery via surface hydrophilic modification of chitosan copolymer based self-assembly polyelectrolyte nanocomplex

Liu, Chang; Kou, Yongqiang; Zhang, Xin; Dong, Wei; Cheng, Hongbo; Mao, Shirui

doi:10.1016/j.ijpharm.2018.10.068

Cited by 71 publications

(19 citation statements)

References 46 publications

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“…The system was engineered by using a chitosan hydrogel whose degradation allowed the leak out of insulin microspheres, enabling an extended insulin delivery. Liu et al obtained a polyelectronic nanocomplex (PEC) for insulin delivery by synthesis of chitosan-g-polyethylene glycol monomethyl ether (CS-g-mPEG) copolymers with various mPEG graft concentrations [106]. They studied the hypoglycemic effect by in vivo oral administration.…”

Section: Glucose-responsive Nanomaterialsmentioning

confidence: 99%

Smart Nanomaterials for Biomedical Applications—A Review

Aflori¹

2021

Nanomaterials

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Recent advances in nanotechnology have forced the obtaining of new materials with multiple functionalities. Due to their reduced dimensions, nanomaterials exhibit outstanding physio-chemical functionalities: increased absorption and reactivity, higher surface area, molar extinction coefficients, tunable plasmonic properties, quantum effects, and magnetic and photo properties. However, in the biomedical field, it is still difficult to use tools made of nanomaterials for better therapeutics due to their limitations (including non-biocompatible, poor photostabilities, low targeting capacity, rapid renal clearance, side effects on other organs, insufficient cellular uptake, and small blood retention), so other types with controlled abilities must be developed, called “smart” nanomaterials. In this context, the modern scientific community developed a kind of nanomaterial which undergoes large reversible changes in its physical, chemical, or biological properties as a consequence of small environmental variations. This systematic mini-review is intended to provide an overview of the newest research on nanosized materials responding to various stimuli, including their up-to-date application in the biomedical field.

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Section: Glucose-responsive Nanomaterialsmentioning

confidence: 99%

Smart Nanomaterials for Biomedical Applications—A Review

Aflori¹

2021

Nanomaterials

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“…We recently assembled micelles from amphiphilic block copolymers with a PEG corona (Figure 1ai) and demonstrated that these micelles penetrated the mucus in a microfluidic setup (Figure 1aii) and in Caco‐2/HT29‐MTX‐E12 co‐cultures within 10 min (Figure 1aiii). 14 Copolymer of PEG and chitosan complexed with insulin showed PEG‐graft ratio dependent mucus permeation in rat intestine 15…”

Section: Mucopenetrationmentioning

confidence: 99%

Recent Advancements in Using Polymers for Intestinal Mucoadhesion and Mucopenetration

Taipaleenmäki

Städler

2020

Macromolecular Bioscience

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Oral administration of actives is the most desired form of delivery, but the formulations need to overcome a variety of barriers including the intestinal mucus. This feature article summarizes the developments from the past 2–3 years in this context focusing on polymer‐based formulations. The progress in assembling mucopenetrating nanoparticles is outlined considering coatings using noninteracting polymers as well as virus‐like particles and charge‐shifting particles. Next, polymers and their modification to enhance mucoadhesion are discussed, followed by providing examples of double‐encapsulation systems that aim to combine mucopenetration with mucoadhesion in the same formulation. Finally, a short outlook is provided highlighting a few of the most pressing challenges to address.

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“…Insulin-loaded CS-Dz13Scr nanoparticles were manufactured by vortex-assisted complex coacervation [65][66][67]. In this study, insulin stock solution (20.9 µM) was prepared by dissolving the powder in Milli-Q water, followed by subsequent dilution to 100 nM by Milli-Q water.…”

Section: Preparation Of Insulin-loaded Cs-dz13scr Nanoparticlesmentioning

confidence: 99%

Development of orally administered insulin-loaded polymeric-oligonucleotide nanoparticles: statistical optimization and physicochemical characterization

Wong

Martinez

Zhao

et al. 2020

Drug Development and Industrial Pharmacy

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Introduction: Therapeutic peptides are administered via parenteral route due to poor absorption in the gastrointestinal (GI) tract, instability in gastric acid and GI enzymes. Polymeric drug delivery systems have achieved significant interest in pharmaceutical research due to its feasibility in protecting proteins, tissue targeting and controlled drug release pattern. Materials and Methods:In the present study, the size, polydispersity index and zeta potential of insulin-loaded nanoparticles were characterized by dynamic light scattering and laser doppler micro-electrophoresis. The main and interaction effects of chitosan concentration and Dz13Scr concentration on the physicochemical properties of the prepared insulin-loaded nanoparticles (size, polydispersity index and zeta potential) were evaluated statistically using Analysis of Variance. A robust procedure of reversed-phase high-performance liquid chromatography was developed to quantify insulin release in simulated GI buffer. Results and Discussion:We reported on the effect of two independent parameters, including polymer concentration and oligonucleotide concentration, on the physical characteristics of particles.Chitosan concentration was significant in predicting the size of insulin-loaded CS-Dz13Scr particles.In terms of zeta potential, both chitosan concentration and squared term of chitosan were significant factors that affect the surface charge of particles, which was attributed to the availability of positively-charged amino groups during interaction with negatively-charged Dz13Scr. The excipients used in this study could fabricate nanoparticles with negligible toxicity in GI cells and skeletal muscle cells. The developed formulation could conserve the physicochemical properties after being stored for 1 month at 4 o C. Conclusion:The obtained results revealed satisfactory results for insulin-loaded CS-Dz13Scr nanoparticles (159.3 nm, pdi 0.331, -1.08 mV). No such similar study has been reported to date to identify the main and interactive significance of the above parameters for the characterization of insulin-loaded polymeric-oligonucleotide nanoparticles. This research is of importance for the understanding and development of protein-loaded nanoparticles for oral delivery.

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Enhanced oral insulin delivery via surface hydrophilic modification of chitosan copolymer based self-assembly polyelectrolyte nanocomplex

Cited by 71 publications

References 46 publications

Smart Nanomaterials for Biomedical Applications—A Review

Smart Nanomaterials for Biomedical Applications—A Review

Recent Advancements in Using Polymers for Intestinal Mucoadhesion and Mucopenetration

Development of orally administered insulin-loaded polymeric-oligonucleotide nanoparticles: statistical optimization and physicochemical characterization

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