Enhanced osteoblastic cell response on zirconia by bio-inspired surface modification

Liu, Yen-Ting; Lee, Tzer Min; Lui, Truan-Sheng

doi:10.1016/j.colsurfb.2013.01.023

Cited by 53 publications

(33 citation statements)

References 35 publications

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“…Indeed, we have shown PDA-mediated stem cell responses, such as an increase in cell proliferation and osteogenic differentiation of PDLSCs. Recent studies reported that PDA-coated zirconia exhibited better cyto-compatibility, including increased cell spreading and proliferation, than did uncoated specimens [28]. In addition, the osteogenic differentiation of human MSCs was increased more when cells were cultured on PDA-coated poly (L-lactide) (PLLA) fibers than those cultured on non-coated PLLA [29].…”

Section: Discussionmentioning

confidence: 99%

Increased Osteogenic Differentiation of Periodontal Ligament Stem Cells on Polydopamine Film Occurs via Activation of Integrin and PI3K Signaling Pathways

Lee

An³

et al. 2014

Cell Physiol Biochem

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Background/Aims: Mussel-inspired polydopamine (PDA) is known to be an effective bioadhesive and bioactive material for controlling stem cell fate, which is important in stem cell-based regenerative medicine; however, the effect of PDA on osteogenic differentiation of periodontal ligament stem cells (PDLSCs) is not fully understood. In this study, we investigated the osteoinductive effect of PDA on PDLSCs and examined how this phenomenon is encouraged. Methods: Osteogenic induction of PDLSCs was established by culturing cells on PDA film or on an uncoated polystyrene surface as a control. Osteogenic differentiation of PDLSCs was assessed by measurement of intracellular calcium levels and alkaline phosphatase (ALP) activity as well as by evaluation of protein expression of osteocalcin (OCN), osterix (OSX), and runt-related transcription factor 2 (RUNX2). Results: The PDLSCs cultured on PDA film showed higher osteogenic activity than those on the control surface. Moreover, PDLSCs on PDA film expressed increased levels of the integrin adhesion receptors integrin α5 and β1 compared to control cells. Expression of one isoform of the intracellular signaling protein phosphatidylinositol-3-kinase (PI3K), p110γ, was increased in PDLSCs on PDA film in a PDA dose-dependent manner. This signaling protein was found to interact with integrin β1, demonstrating integrin-linked PI3K activation in response to PDA. Finally, the blockage of PI3K reduced the PDA-induced osteogenic activity of PDLSCs. Conclusion: our findings suggest that the bioadhesive PDA stimulates osteogenic differentiation of PDLSCs via activation of the integrin α5/β1 and PI3K signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Increased Osteogenic Differentiation of Periodontal Ligament Stem Cells on Polydopamine Film Occurs via Activation of Integrin and PI3K Signaling Pathways

Lee

An³

et al. 2014

Cell Physiol Biochem

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“…In general, the outstanding adhesive properties of MAPs are attributed to 3,4-dihydroxyphenylalanine (DOPA), which is the hydroxylated form of tyrosine. 16 Aside from its adhesive role as a protein constituent, several reports show that the DOPA molecule shows osteoinductive activity in vitro [17][18][19] and in vivo.…”

Section: 14mentioning

confidence: 99%

Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

et al. 2015

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“…mecânicas (elevada resistência à flexão, alta tenacidade à fratura, alto módulo de elasticidade e alta resistência ao desgaste), alta estabilidade química, biocompatibilidade e excelente aparência estética [4,5], o que o torna um material bastante versátil em suas características e possibilita seu uso em várias aplicações tecnológicas, tais como revestimento refratário, material semicondutor [6], membranas [7], próteses e implantes dentários [8], implantes ortopédicos [9], materiais biológicos [10], pigmentos, dispositivos piezoeléctricos, sensores de oxigênio [11], suporte de catalisadores [12], assim como catalisador sulfatado para produção de biodiesel [13].…”

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Preparação e caracterização de catalisadores ácidos de zircônia sulfatada para aplicação na esterificação do óleo de algodão

et al. 2017

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INTRODUÇÃOA zircônia (ZrO 2 ) apresenta transformações polimórficas e sua estrutura cristalina pode sofrer alterações na medida em que variações ocorrem na temperatura, sem alteração da composição química. As mudanças cristalinas mais comuns são: monoclínica (m), tetragonal (t) e cúbica [1][2][3][4], apesar de outra fase de transição, não majoritária, possa ocorrer durante o processo de síntese, denominada de ortorrômbica [1,3,4]. O ZrO 2 é um material usado em diferentes setores tecnológicos, como na engenharia, na medicina, na odontologia e na catálise, devido às suas propriedades

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Enhanced osteoblastic cell response on zirconia by bio-inspired surface modification

Cited by 53 publications

References 35 publications

Increased Osteogenic Differentiation of Periodontal Ligament Stem Cells on Polydopamine Film Occurs via Activation of Integrin and PI3K Signaling Pathways

Increased Osteogenic Differentiation of Periodontal Ligament Stem Cells on Polydopamine Film Occurs via Activation of Integrin and PI3K Signaling Pathways

Engineered mussel bioglue as a functional osteoinductive binder for grafting of bone substitute particles to accelerate in vivo bone regeneration

Preparação e caracterização de catalisadores ácidos de zircônia sulfatada para aplicação na esterificação do óleo de algodão

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