Enhanced osteochondral regeneration with a 3D-Printed biomimetic scaffold featuring a calcified interfacial layer

Wu, Di; Zheng, Kaiwen; Yin, Wenjing; Hu, Bin; Yu, Mingzhao; Yu, Qingxiao; Wei, Xiaojuan; Deng, Jue; Zhang, Changqing

doi:10.1016/j.bioactmat.2024.03.004

Cited by 2 publications

(4 citation statements)

References 53 publications

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“…Consequently, the clear biological distinctions observed in conventional scaffolds become less discernible, resulting in reduced efficacy of OC regeneration, including issues such as heterotopic ossification. 251 Notably, there exists a critically important calcified interface between native AC and subchondral bone. 252 Based on bilayer scaffolds, it is imperative to fabricate trilayer scaffolds by integrating a calcified interface to overcome the limitations observed with bilayer scaffolds.…”

Section: Endogenous Tissue Engineering For Ac and Oc Regenerationmentioning

confidence: 99%

“…Furthermore, the middle layer serves as a barrier, inhibiting vascular invasion into the AC and preventing the calcification of hyaluronic AC. 251 Due to the risk of delamination associated with multilayered scaffolds, there is a growing interest in OC tissue engineering for the development of monolithic scaffolds with a gradient structure. 253 Furthermore, a highly organized structure featuring varying degrees of mineralization and pore sizes must be accurately mimicked to facilitate OC regeneration.…”

Section: Endogenous Tissue Engineering For Ac and Oc Regenerationmentioning

confidence: 99%

“…However, when bilayered scaffolds are exposed to dynamic physiological environments, the distinct boundaries between their layers tend to blur over time due to substance diffusion or cell migration. Consequently, the clear biological distinctions observed in conventional scaffolds become less discernible, resulting in reduced efficacy of OC regeneration, including issues such as heterotopic ossification . Notably, there exists a critically important calcified interface between native AC and subchondral bone .…”

Section: Endogenous Tissue Engineering For Ac and Oc Regenerationmentioning

confidence: 99%

“…This structure effectively physically and functionally separates the top layer (tannic-acid-mixed GelMA for AC repair) from the bottom layer (porous PCL for subchondral bone repair). Furthermore, the middle layer serves as a barrier, inhibiting vascular invasion into the AC and preventing the calcification of hyaluronic AC . Due to the risk of delamination associated with multilayered scaffolds, there is a growing interest in OC tissue engineering for the development of monolithic scaffolds with a gradient structure .…”

Section: Endogenous Tissue Engineering For Ac and Oc Regenerationmentioning

confidence: 99%

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Endogenous Tissue Engineering for Chondral and Osteochondral Regeneration: Strategies and Mechanisms

Zhang,

Chen,

Sun

et al. 2024

ACS Biomater. Sci. Eng.

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Increasing attention has been paid to the development of effective strategies for articular cartilage (AC) and osteochondral (OC) regeneration due to their limited selfreparative capacities and the shortage of timely and appropriate clinical treatments. Traditional cell-dependent tissue engineering faces various challenges such as restricted cell sources, phenotypic alterations, and immune rejection. In contrast, endogenous tissue engineering represents a promising alternative, leveraging acellular biomaterials to guide endogenous cells to the injury site and stimulate their intrinsic regenerative potential. This review provides a comprehensive overview of recent advancements in endogenous tissue engineering strategies for AC and OC regeneration, with a focus on the tissue engineering triad comprising endogenous stem/ progenitor cells (ESPCs), scaffolds, and biomolecules. Multiple types of ESPCs present within the AC and OC microenvironment, including bone marrow-derived mesenchymal stem cells (BMSCs), adipose-derived mesenchymal stem cells (AD-MSCs), synovial membrane-derived mesenchymal stem cells (SM-MSCs), and AC-derived stem/progenitor cells (CSPCs), exhibit the ability to migrate toward injury sites and demonstrate pro-regenerative properties. The fabrication and characteristics of scaffolds in various formats including hydrogels, porous sponges, electrospun fibers, particles, films, multilayer scaffolds, bioceramics, and bioglass, highlighting their suitability for AC and OC repair, are systemically summarized. Furthermore, the review emphasizes the pivotal role of biomolecules in facilitating ESPCs migration, adhesion, chondrogenesis, osteogenesis, as well as regulating inflammation, aging, and hypertrophy-critical processes for endogenous AC and OC regeneration. Insights into the applications of endogenous tissue engineering strategies for in vivo AC and OC regeneration are provided along with a discussion on future perspectives to enhance regenerative outcomes.

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Section: Endogenous Tissue Engineering For Ac and Oc Regenerationmentioning

confidence: 99%

Section: Endogenous Tissue Engineering For Ac and Oc Regenerationmentioning

confidence: 99%

Section: Endogenous Tissue Engineering For Ac and Oc Regenerationmentioning

confidence: 99%

Section: Endogenous Tissue Engineering For Ac and Oc Regenerationmentioning

confidence: 99%

See 2 more Smart Citations

Endogenous Tissue Engineering for Chondral and Osteochondral Regeneration: Strategies and Mechanisms

Zhang,

Chen,

Sun

et al. 2024

ACS Biomater. Sci. Eng.

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Reduced graphene oxide loaded with tetrahedral framework nucleic acids for combating orthodontically induced root resorption

Yuan,

Huang,

Chen

et al. 2024

J Nanobiotechnol

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Root resorption occurs outside the root or within the root canal. Regardless of its region, root resorption is irreversible and in severe cases, may even cause tooth loss. Clinically, the external surface root resorption is usually a side effect of orthodontic tooth movement. However, it is frustrating to note that there are almost no effective treatment strategies for orthodontically induced root resorption (OIRR) due to the complexity and ambiguity of etiology. In the current study, we successfully fabricated a delivery complex, reduced graphene oxide nanosheet loading with tetrahedral framework nucleic acids (tFNAs-rGO) through self-assembly. No significant cytotoxicity or organ-toxicity of the tFNAs-rGO complex was observed in cell counting kit-8 assay (CCK-8) and hematoxylin-eosin (HE) staining. Histological staining such as tartrate-resistant acid phosphatase (TRAP) staining and Micro-CT three-dimensional reconstruction were employed to explore the dynamic changes of root and peri-root tissues in OIRR mice. In vitro, we developed an induction microenvironment to testify the effects of the tFNAs-rGO delivery complex on periodontal ligament cells (PDLCs) and macrophages by quantitative RT-PCR, western blot, and immunofluorescence staining. The data showed the reduced the region of root resorption and downregulated osteoclastic activity in OIRR by the tFNAs-rGO complex treatment. Furthermore, our study demonstrated that the tFNAs-rGO delivery complex enhanced osteogenic differentiation of PDLCs and facilitated M2-phenotype polarization of macrophages to ameliorate OIRR. Collectively, the insight into the nanoscale dual-functional tFNAs-rGO delivery complex regulating the cell populations of PDLCs and macrophages in the root resorption remodeling proposes a promising therapeutic strategy for orthodontically induced root resorption. Supplementary Information The online version contains supplementary material available at 10.1186/s12951-024-02988-2.

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Enhanced osteochondral regeneration with a 3D-Printed biomimetic scaffold featuring a calcified interfacial layer

Cited by 2 publications

References 53 publications

Endogenous Tissue Engineering for Chondral and Osteochondral Regeneration: Strategies and Mechanisms

Endogenous Tissue Engineering for Chondral and Osteochondral Regeneration: Strategies and Mechanisms

Reduced graphene oxide loaded with tetrahedral framework nucleic acids for combating orthodontically induced root resorption

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