Enhanced parylene-C fluorescence as a visual marker for neuronal electrophysiology applications

Zhang, Lingqian; Wei, Mengping; Shao, Linbo; Li, Mingli; Dai, Wanwei; Cui, Yaxuan; Li, Zhihong; Zhang, Chen; Wang, Wei

doi:10.1039/c8lc00804c

Cited by 7 publications

(4 citation statements)

References 40 publications

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“…The most important findings are that interconversion to a long-lived emission state is only possible for the nanofibers and that this state is more accessible to the PPX-CH 2 NH 2 nanofibers than to the PPX-NH 2 (as confirmed through fluorescence lifetime traces and the amount of long-lived emission relative to total emission). , Some reports indicate that the surfaces of organic nanostructures are capable of exhibiting emissive properties that are markedly different than that of bulk or internalized materials. , Given the high surface-to-volume ratio of these nanofiber films, this may also serve to explain the emergence of the phosphorescence observed in these systems, especially if the LC template during growth provides local environments not found in the bulk. These findings open the path for potential PPX usage in the areas of optoelectronics or bioimaging . While the re-emergence of emission with polymerization is interesting, the evolution of a long-lived emission from nanostructuring of the polymer is doubly so.…”

Section: Resultsmentioning

confidence: 93%

“…These findings open the path for potential PPX usage in the areas of optoelectronics or bioimaging. 50 While the reemergence of emission with polymerization is interesting, the evolution of a long-lived emission from nanostructuring of the polymer is doubly so. It was also observed that short-lived (ns regime) emission could be observed in the bulk polymer, though no evidence a long-lived emission was found in the unstructured film.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Emergence of Structural Phosphorescence in Free-Standing, Laterally Organized Polymer Nanofiber Membranes

Wawryszyn

Wilhelm

Kim

et al. 2023

ACS Appl. Polym. Mater.

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In the past decade, the phosphorescence of metal-free confined polymer structures has generated interest in optoelectronics due to their sustainability, low toxicity, and deployment in sensing applications. Herein, a free-standing array of laterally organized nanofibers was prepared via templated chemical vapor deposition polymerization into a liquid crystalline (LC) phase, and their optical properties were compared to compositionally identical films. The fibers converge into laterally aligned membranes that maintain a high internal order despite the surfaces of the membranes remaining uniform and closed. The membranes consisted of hourglass-shaped nanofibers with features in the micro- and nanometer regime. Free-standing nanofiber membranes differ from polymer films of equivalent chemical composition in several key features: (1) Anisotropic growth of polymer nanofibers with constraint and compliance to an LC template, (2) a high surface-to-volume ratio, and (3) the occurrence of a long-lived emission in the blue region, which persists for multiple seconds after excitation. This study constitutes the first report of long-lived emission from solid-state poly(p-xylylenes) nanofibers. Prospective applications of morphologically controlled polymer arrays with structural luminescence include organic sensors and optoelectronic devices.

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Section: Resultsmentioning

confidence: 93%

Section: ■ Results and Discussionmentioning

confidence: 99%

Emergence of Structural Phosphorescence in Free-Standing, Laterally Organized Polymer Nanofiber Membranes

Wawryszyn

Wilhelm

Kim

et al. 2023

ACS Appl. Polym. Mater.

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“…2A). A transparent test platform formed with parylene-C and without microelectrodes or microdevices facilitates imaging by confocal microscopy (22), as in Fig. 2B (note S2).…”

Section: Resultsmentioning

confidence: 99%

Three-dimensional, multifunctional neural interfaces for cortical spheroids and engineered assembloids

et al. 2021

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Three-dimensional (3D), submillimeter-scale constructs of neural cells, known as cortical spheroids, are of rapidly growing importance in biological research because these systems reproduce complex features of the brain in vitro. Despite their great potential for studies of neurodevelopment and neurological disease modeling, 3D living objects cannot be studied easily using conventional approaches to neuromodulation, sensing, and manipulation. Here, we introduce classes of microfabricated 3D frameworks as compliant, multifunctional neural interfaces to spheroids and to assembloids. Electrical, optical, chemical, and thermal interfaces to cortical spheroids demonstrate some of the capabilities. Complex architectures and high-resolution features highlight the design versatility. Detailed studies of the spreading of coordinated bursting events across the surface of an isolated cortical spheroid and of the cascade of processes associated with formation and regrowth of bridging tissues across a pair of such spheroids represent two of the many opportunities in basic neuroscience research enabled by these platforms.

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“…A number of synthetic polymers, such as Parylene, 141,142 PDMS, 143 PI 144,145 and silicone 46 also showed good biocompatibility and bioactivity with neural and brain tissue. These materials have a wide range of compositions and properties, thus allowing the tailored designs of the electrode for different applications.…”

Section: Synthetic Polymer Materialsmentioning

confidence: 99%

Bioactive polymer-enabled conformal neural interface and its application strategies

Niu

Hsiao

et al. 2023

Mater. Horiz.

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Enhanced parylene-C fluorescence as a visual marker for neuronal electrophysiology applications

Abstract: This work presented a simple annealing method to significantly enhance the fluorescence of parylene-C and achieve sufficient intensity as a visual marker.

Cited by 7 publications

References 40 publications

Emergence of Structural Phosphorescence in Free-Standing, Laterally Organized Polymer Nanofiber Membranes

Emergence of Structural Phosphorescence in Free-Standing, Laterally Organized Polymer Nanofiber Membranes

Three-dimensional, multifunctional neural interfaces for cortical spheroids and engineered assembloids

Bioactive polymer-enabled conformal neural interface and its application strategies

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