Enhanced Performance of Colorimetric Biosensing on Paper Microfluidic Platforms Through Chemical Modification and Incorporation of Nanoparticles

Abstract

This chapter describes two different methodologies used to improve the analytical performance of colorimetric paper-based biosensors. Microfluidic paper-based analytical devices (μPADs) have been produced by a stamping process and CO laser ablation and modified, respectively, through an oxidation step and incorporation of silica nanoparticles on the paper structure. Both methods are employed in order to overcome the largest problem associated with colorimetric detection, the heterogeneity of the color distribution in the detection zones. The modification steps are necessary to improve the interaction between the paper surface and the selected enzymes. The enhanced performance has ensured reliability for quantitative analysis of clinically relevant compounds.

Detection of toxin B ofClostridium difficilebased on immunomagnetic separation and aptamer‐mediated colorimetric assay

“…The above mentioned limitations can be addressed by a rapid and sensitive colorimetric assay (CA) via a colour change (Khan et al 2015;Chen et al 2017;Lin et al 2017). Since this technology did not require trained personnel and expensive equipment, CA has been taken into account as one of the best candidate for point-of-care test (Gabriel et al 2017;Lin et al 2018).…”

“…Since this technology did not require trained personnel and expensive equipment, CA has been taken into account as one of the best candidate for point‐of‐care test (Gabriel et al . 2017; Lin et al . 2018).…”