Enhanced photocatalytic disinfection of Escherichia coli K-12 by porous g-C3N4 nanosheets: Combined effect of photo-generated and intracellular ROSs

He, Nannan; Cao, Shihai; Zhang, Lihao; Tian, Zhidan; Chen, Huan; Jiang, Fang

doi:10.1016/j.chemosphere.2019.07.007

Cited by 30 publications

(9 citation statements)

References 51 publications

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“…coli. A similar observation has been reported previously . With the introduction of 0.2 mM PI into the reaction system, 0.70 log reduction of viable E.…”

Section: Resultssupporting

confidence: 89%

“…A similar observation has been reported previously. 31 With the introduction of 0.2 mM PI into the reaction system, 0.70 log reduction of viable E. coli cells was achieved under the dark condition in 40 min with k obs = (0.5 ± 0.1) × 10 −3 min −2 . The observation suggested that PI contained limited disinfection performance under dark condition, which agreed with the previous study.…”

Section: ■ Results and Discussionmentioning

confidence: 98%

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Catalyst-Free Periodate Activation by Solar Irradiation for Bacterial Disinfection: Performance and Mechanisms

Liu

Dong

et al. 2022

Environ. Sci. Technol.

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Periodate (PI)-based advanced oxidation process has recently attracted great attention in the water treatment processes. In this study, solar irradiation was used for PI activation to disinfect waterborne bacteria. The PI/solar irradiation system could inactivate Escherichia coli below the limit of detection (LOD, 10 CFU mL–1) with initial concentrations of 1 × 106, 1 × 107, and 1 × 108 CFU mL–1 within 20, 40, and 100 min, respectively. •O2 – and •OH radicals contributed to the bacterial disinfection. These reactive radicals could attack and penetrate the cell membrane, thereby increasing the amount of intracellular reactive oxygen species and destroying the intracellular defense system. The damage of the cell membrane caused the leakage of intracellular K+ and DNA (that could be eventually degraded). Excellent bacterial disinfection performance in PI/solar irradiation systems was achieved in a wide range of solution pH (3–9), with coexisting humic acid (0.1–10 mg L–1) and broad solution ionic strengths (15–600 mM). The PI/solar irradiation system could also efficiently inactivate Gram-positive Bacillus subtilis. Moreover, PI activated by natural sunlight irradiation could inactivate 1 × 107 CFU mL–1 viable E. coli below the LOD in the river and sea waters with a working volume of 1 L in 40 and 50 min, respectively. Clearly, the PI/solar system could be potentially applied to disinfect bacteria in water.

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“…coli. A similar observation has been reported previously . With the introduction of 0.2 mM PI into the reaction system, 0.70 log reduction of viable E.…”

Section: Resultssupporting

confidence: 89%

Section: ■ Results and Discussionmentioning

confidence: 98%

Catalyst-Free Periodate Activation by Solar Irradiation for Bacterial Disinfection: Performance and Mechanisms

Liu

Dong

et al. 2022

Environ. Sci. Technol.

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“…The band gap is around 2.7 eV, which allows the absorption of visible light (λ ≥ 420 nm) . Moreover, various morphologies such as quantum dots, nanosheets, and three-dimensional porous structures can be obtained via the self-assembly of precursors or the postetching process. − Until now, graphitic carbon nitride nanomaterials have demonstrated disinfection properties under the irradiation of visible light. ,,− The major challenge is that the quantum efficiency is still rather low due to the fast recombination of photoexcited charge carriers. Doping with various elements or carbon-ring structures is an important way to separate the electron–hole pairs. − However, relatively little research has been conducted on the synchronous photothermal effect toward efficient visible-light-activated water disinfection.…”

Section: Introductionmentioning

confidence: 99%

Harnessing Photocatalytic and Photothermal Effects of C-Doped Graphitic Carbon Nitride for Efficient Bacterial Disinfection

Lyu

Jia

et al. 2021

ACS Appl. Bio Mater.

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In this work, the photocatalytic and photothermal effects of carbon-ring-doped graphitic carbon nitride materials against bacteria were systematically studied in a dispersed solution and on a membrane. C-doped graphitic carbon nitride materials C-CN 0.15, 1.5, and 7.5 were synthesized by mixing urea precursor with 0.15, 1.5, and 7.5 wt % glucose. With the increase in the doping level, the photothermal effect was clearly enhanced while the generation of reactive oxygen species (ROS) was slightly inhibited. With exposure to irradiation under a 100 mW cm −2 Xeon lamp with a cutoff filter (λ ≥ 420 nm), the ROS concentration of C-CN 1.5 increased 30% in the dispersed solution and its temperature increased about 10 °C in the dispersed solution and on the membrane compared to that of pristine carbon nitride. As a result, the bactericidal activity of C-CN 1.5 was improved by an order of magnitude in the dispersed solution and more than 2 orders of magnitude on the membrane immersed in a solution at 40 °C. To investigate the fundamental light absorption process on the membrane, an optical model using the finite-difference time-domain method was developed based on the topography of the membrane. The simulation results may explain that although C-CN produces more ROS in a solution; however, with a larger extinction coefficient, the power absorption is lower near the surface of the membrane. The ROS production is therefore inhibited and the bactericidal activity is dominated by the photothermal effect. Our experimental and simulation results provide a basis for designing high-performance photoactive disinfection materials and surfaces.

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“…Figure B indicates that in g-C 3 N 4 -treated MCF-7 cells there is a lowered accumulation of the mitotracker red dye. Previous studies reported ROS-mediated stress, mitochondrial functional loss, and damage to macromolecules such as DNA in cells treated with graphene and its derivatives. , Thus, to understand the signaling pathways regulated by g-C 3 N 4 in MCF-7 cells, we performed DNA microarray analysis to identify the global transcriptomic signature of g-C 3 N 4 treatment in epithelial cells.…”

Section: Resultsmentioning

confidence: 99%

Graphitic Carbon Nitride Causes Widespread Global Molecular Changes in Epithelial and Fibroblast Cells

et al. 2021

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For scaffold and imaging applications, nanomaterials such as graphene and its derivatives have been widely used. Graphitic carbon nitride (g-C 3 N 4 ) is among one such derivative of graphenes, which draws strong consideration due to its physicochemical properties and photocatalytic activity. To use g-C 3 N 4 for biological applications, such as molecular imaging or drug delivery, it must interact with the epithelium, cross the epithelial barrier, and then come in contact with the extracellular matrix of the fibroblast cells. Thus, it becomes essential to understand its molecular mechanism of action. Hence, in this study, to understand the molecular reprogramming associated with g-C 3 N 4 , global gene expression using DNA microarrays and proteomics using tandem mass tag (TMT) labeling and mass spectrometry were performed in epithelial and fibroblast cells, respectively. Our results showed that g-C 3 N 4 can cross the epithelial barrier by regulating the adherens junction proteins. Further, using g-C 3 N 4 –PDMS scaffolds as a mimic of the extracellular matrix for fibroblast cells, the common signaling pathways were identified between the epithelium and fibroblast cells. These pathways include Wnt signaling, integrin signaling, TGF-β signaling, cadherin signaling, oxidative stress response, ubiquitin proteasome pathway, and EGF receptor signaling pathways. These altered signature pathways identified could play a prominent role in g-C 3 N 4 -mediated cellular interactions in both epithelial and fibroblast cells. Additionally, β catenin, EGFR, and MAP2K2 protein–protein interaction networks could play a prominent role in fibroblast cell proliferation. The findings could further our knowledge on g-C 3 N 4 -mediated alterations in cellular molecular signatures, enabling the potential use of these materials for biological applications such as molecular imaging and drug delivery.

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Enhanced photocatalytic disinfection of Escherichia coli K-12 by porous g-C3N4 nanosheets: Combined effect of photo-generated and intracellular ROSs

Cited by 30 publications

References 51 publications

Catalyst-Free Periodate Activation by Solar Irradiation for Bacterial Disinfection: Performance and Mechanisms

Catalyst-Free Periodate Activation by Solar Irradiation for Bacterial Disinfection: Performance and Mechanisms

Harnessing Photocatalytic and Photothermal Effects of C-Doped Graphitic Carbon Nitride for Efficient Bacterial Disinfection

Graphitic Carbon Nitride Causes Widespread Global Molecular Changes in Epithelial and Fibroblast Cells

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