a b s t r a c tIn this paper, the photocatalytic inactivation of dry spores of Bacillus subtilis dispersed on TiO 2 films and irradiated with UV-A radiation (2.44-0.29 mW cm −2 ) was studied. Experimental results indicate a minor reduction in the number of viable spores (69% of inactivation after 48 h of irradiation) when they were irradiated with UV-A radiation on borosilicate glass plates (without TiO 2 ), confirming the resistance of spores to UV-A radiation. However, the number of viable spores significantly decreased when they were irradiated with UV-A under similar conditions but on plates coated with TiO 2 (99.88% of inactivation after 24 h of irradiation), showing that dry spores of B. subtilis are vulnerable to photocatalytic inactivation. A simplified scheme was proposed to model the photocatalytic inactivation of B. subtilis, from which a kinetic expression was derived. Since the rate of photocatalytic inactivation is strongly dependent on the flux of the UV radiation on the TiO 2 films, the radiation field was modeled by means of CFD software. Experimental results of spore inactivation were fitted with the derived kinetic expression, showing the inactivation rate has a square root dependence on the radiation flux reaching the photocatalyst film.