Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize

Bandyopadhyay, Anindya; Datta, Karabi; Zhang, J.; Yang, Woonho; Raychaudhuri, Sarmistha Sen; Miyao, Mitsue; Datta, Swapan K.

doi:10.1016/j.plantsci.2007.02.016

Cited by 81 publications

(28 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…No apparent differences in phenotype were observed in any of the transgenic plants as compared to their wildtype parents. This result is similar to other reports that over 90% transgenic rice plants carrying the same construct in different cultivars exhibited normal phenotype as the wild-type origin (Ku et al 1999;Bandyopadhyay et al 2007).…”

Section: Discussionsupporting

confidence: 92%

“…These studies revealed an enhanced drought tolerance of transgenic rice plants expressing high levels of maize PEPC by more efficiently scavenging reactive oxyradicals. Moreover, the same construct has been used to transform the indica rice line IR68899B and transformants showed enhanced photosynthesis rate under high temperature conditions (Bandyopadhyay et al 2007). Most recently, transgenic wheat plants were transformed with pepc cDNA and showed about fourfold higher PEPC activity, relative to the wild-type wheat (Zhang et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Most of the transgenic rice plants generated showed high-level expression of the maize pepc gene and exhibited reduced sensitivity of photosynthesis to O 2 inhibition (Ku et al 1999). Later, the same construct was used to transform indica rice IR68899B using a bombardment method, and the transformants showed enhancement in photosynthesis rate under high temperature (Bandyopadhyay et al 2007). Now, it is generally agreed that overexpression of a single maize pepc gene encoding PEPC protein or even quadruple transformants overexpressing all four key enzymes in the C 4 photosynthetic pathway [i.e., PEPC, pyruvate, orthophosphate dikinase (PPDK), NADP-malate dehydrogenase (NADP-MDH), NADP-malic enzyme (NADP-ME)] into rice plants do not show dramatic improvements in photosynthesis under normal conditions (Taniguchi et al 2008;Kajala et al 2011;Miyao et al 2011).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transformation and characterization of transgenic rice and Cleome spinosa plants carrying the maize phosphoenolpyruvate carboxylase genomic DNA

Chen

Tsai

et al. 2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transformation and characterization of transgenic rice and Cleome spinosa plants carrying the maize phosphoenolpyruvate carboxylase genomic DNA

Chen

Tsai

et al. 2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…The photosynthetic process involves CO 2 fixation and consequent production of carbohydrates and O 2 delivery, using an intermediary light energy. Higher plants are classified as C 3 , C 4 and CAM metabolism in relation to CO 2 fixation (Bandyopadhyay et al 2007). Phaseolus vulgaris is an example of a C 3 species (Santos et al 2009), because CO 2 is catalysed into 3-phosphoglyglycerate by the enzyme RU-BISCO (ribulose-1,5 bisphosphate carboxylase/ oxygenase) (Furbank & Taylor 1995).…”

Section: Introductionmentioning

confidence: 99%

Relationships between leaf pigments and photosynthesis in common bean plants infected by anthracnose

Lobato

Gonçalves‐Vidigal

Filho

et al. 2010

New Zealand Journal of Crop and Horticultural Science

View full text Add to dashboard Cite

The objective of this study was to: first, evaluate the infection effects caused by Colletotrichum lindemuthianum pathogen (race 2047) on photosynthetic pigments and gas exchanges in Phaseolus vulgaris plants (cv. Mexico 222); and, second, determine infection effects on leaf pigments and their consequences on photosynthesis rate. A completely randomized design with a factorial scheme was used, combining two treatments (control and inoculated) and three evaluation periods (4th, 8th and 12th day). Carotenoid levels presented decreases of 28.3% and 35% during the 8th and 12th day after infection, when control and inoculated plants were compared. Correlation analysis demonstrated the direct relationship between carotenoids and photosynthesis rate (r 00.84). Total chlorophyll in infected plants had progressive reductions of 6.4%, 20.6% and 21.3% on the 4th, 8th and 12th day, respectively, when treated and untreated plants were compared. Total chlorophyll with photosynthesis (r 00.85) also revealed a significant and linear correlation. The photosynthetic rate in infected plants decreased by 22%, 49.9% and 77.3% on the 4th, 8th and 12th days after the inoculation, respectively. Anthracnose infection also induced negative effects concerning stomatal conductance, transpiration, photosynthesis and water use efficiency. Our results demonstrate that leaf pigment reduction as a result of pathogens was the main cause of lower gaseous exchanges in infected plants.

show abstract

“…Ku et al (2001) found that the grain yield was about 10~20% higher in Kitaake with ZmC4Ppc transgenic rice lines under small-scale field trial, as compared with the untransformed plants. Bandyopadhyay et al (2007) reported that overproduction of the maize PEPCase enhanced photosynthesis and increased crop yield of transgenic rice plants. PEPCase was overexpressed in the same intracellular compartment, cytosol, in C 3 plants as in C 4 plants (Miyao, 2003).…”

Section: Introductionmentioning

confidence: 99%

Breeding of Marker-free Indica Three-lines Restorer with ZmC4Ppc by Marker-assisted Selection

Xiao

et al. 2011

JAS

View full text Add to dashboard Cite

Enhancing photosynthetic productivity of rice is always the key goal of researchers by to use exogenous C 4 genes. The full-length of intact maize gene of C 4 -specific PEPCase (ZmC 4 Ppc) is 6781 bp that makes it difficult for marker-assisted selection (MAS) in rice. The specific marker of intact ZmC 4 Ppc was designed and termed as MRpc. MRpc was verified that it was a gene marker because ZmC 4 Ppc could be overexpressed at every developmental stage and it was used for MAS of indica restorer Shuhui881 with ZmC 4 Ppc and FPM881was gotten, which was tested for genetic background, PEPCase activity, net photosynthetic rate (P n ), general combining ability (GCA) and specific combining ability (SCA). The results indicated that FPM881, which similarity of genetic background had arrived at above 95%, showed a higher PEPCase activity and net photosynthetic rate than those of control. Analysis of yield components showed that some of the progenies carrying ZmC 4 Ppc had better GCA and SCA than those of the control. Then, six lines of FPM881 were planted in larger population by selfing and gotten selectable-marker free plants with target gene using MRpc and hpt primers. Our results indicated that introduction of ZmC 4 Ppc via MAS and its stable expression could increase grain yield of hybrid rice.

show abstract

Enhanced photosynthesis rate in genetically engineered indica rice expressing pepc gene cloned from maize

Cited by 81 publications

References 27 publications

Transformation and characterization of transgenic rice and Cleome spinosa plants carrying the maize phosphoenolpyruvate carboxylase genomic DNA

Transformation and characterization of transgenic rice and Cleome spinosa plants carrying the maize phosphoenolpyruvate carboxylase genomic DNA

Relationships between leaf pigments and photosynthesis in common bean plants infected by anthracnose

Breeding of Marker-free Indica Three-lines Restorer with ZmC4Ppc by Marker-assisted Selection

Contact Info

Product

Resources

About