Enhanced phototoxicity of photodynamic treatment by Cx26‐composed GJIC via ROS‐, calcium‐ and lipid peroxide‐mediated pathways

Wu, Dalin; Lin, Tianyu; Bai, Li-Ru; Huang, Jinlan; Zhou, Yan; Zhou, Nan; Zhong, Sheng-Lei; Gao, Shan; Yin, Xiaoxing

doi:10.1002/jbio.201600255

Cited by 15 publications

(30 citation statements)

References 33 publications

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“…Deng‐Pan Wu et al. from Xuzhou Medical University demonstrated the enhanced phototoxicity of photodynamic treatment by connexin 26‐composed gap junctional intercellular communication via ROS‐, calcium‐ and lipid peroxide‐mediated pathways . Weisong Shi et al.…”

Section: Research Interests Indicated By Jbpmentioning

confidence: 99%

Biophotonics in China

Shi

Luo

2017

Journal of Biophotonics

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Biophotonics is a highly interdisciplinary field where physicists, chemists, biologists, physicians and engineers work together to solve the problems appearing in biology and medicine. In China, the Biophotonics discipline is often referred to as Biomedical Photonics, under the first‐level disciplines Biomedical Engineering or Optical Engineering, and was initiated in the late 1990s. Over the past 20 years, biophotonics research in China expanded extraordinarily and has reached the frontiers of the world‐level sciences. This white paper introduces the research groups in the biophotonics field in China, and their representative contributions.

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Section: Research Interests Indicated By Jbpmentioning

confidence: 99%

Biophotonics in China

Shi

Luo

2017

Journal of Biophotonics

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“…Photofrin‐mediated PDT has firstly been approved in 1993 in Canada for the prophylactic treatment of bladder cancer . Our previous studies showed that homotypic Cx32‐ and Cx26‐composed GJIC‐mediated “bystander effect” could enhance the phototoxicity of Photofrin‐mediated PDT . The present study represented the first demonstration of the ability of heterotypic Cx32/Cx26‐composed GJIC to have a “Good Samaritan” effect on HeLa cells following Photofrin‐PDT treatment.…”

Section: Introductionmentioning

confidence: 50%

“…The Western blot assay was utilized as described in our previous studies . Briefly, after protein samples were separated, samples were transferred to nitrocellulose transfer membrane (Excell Bio, China).…”

Section: Methodsmentioning

confidence: 99%

“…The assay was performed according to our previous studies for the measurement of gap junctional function . After donor and receiver cells were grown to 70–100% confluence, donor cells from one well were incubated with 5 µM calcein‐acetoxymethyl ester for 30 min, and then the cells were trypsinized and seeded to the receiver cells at 1:150 donor/receiver ratio for 4 hours at 37°C, and then donor cells were allowed to contact with the monolayer of receiver cells and form GJ.…”

Section: Methodsmentioning

confidence: 99%

“…ELISA assay was performed to detect the levels of PGE 2 and cAMP according to the protocols . Cells at 70–100% confluent were incubated with or without 2.5 µg/ml for 4 hours.…”

Section: Methodsmentioning

confidence: 99%

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Decreased phototoxicity of photodynamic therapy by Cx32/Cx26‐composed GJIC: A “Good Samaritan” effect

Ding

Bai

et al. 2019

Lasers Surg Med

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Background and Objective Photodynamic therapy (PDT) has been widely used to treat malignant tumors. Our previous studies indicated that connexin (Cx) 32‐ and Cx26‐composed gap junctional intercellular communication (GJIC) could improve the phototoxicity of PDT. However, the role of heterotypic Cx32/Cx26‐formed GJIC in PDT phototoxicity is still unknown. Thus, the present study was aimed to investigate the effect of Cx32/Cx26‐formed GJIC on PDT efficacy. Methods CCK8 assay was used to detect cell survival after PDT. Western blot assay was utilized to detect Cx32/Cx26 expression. “Parachute” dye‐coupling assay was performed to measure the function of GJ channels. The intracellular Ca2+ concentrations were determined using flow cytometer. ELISA assay was performed to detect the intracellular levels of PGE2 and cAMP. Results The present study demonstrates there is a Cx32/Cx26‐formed GJIC‐dependent reduction of phototoxicity when cells were exposure to low concentration of Photofrin. Such a protective action is missing at low cell density due to the lack of GJ coupling. Under high‐cell density condition, where there is opportunity for the cells to contact each other and form GJ, suppressing Cx32/Cx26‐formed GJIC by either inhibiting the expression of Cx32/Cx26 or pretreating with GJ channel inhibitor augments PDT phototoxicity after cells were treated with at 2.5 µg/ml Photofrin. The above results suggest that at low Photofrin concentration, the presence of Cx32/Cx26‐formed GJIC may decrease the phototoxicity of PDT, leading to the insensitivity of malignant cells to PDT treatment. The GJIC‐mediated PDT insensitivity was associated with Ca2+ and prostaglandin E2 (PGE2) signaling pathways. Conclusion The present study provides a cautionary note that for tumors expressing Cx32/Cx26, the presence of Cx32/Cx26‐composed GJIC may cause the resistance of tumor cells to PDT. Oppositely, treatment strategies designed to downregulate the expression of Cx32/Cx26 or restrain the function of Cx32/Cx26‐mediated GJIC may increase the sensitivity of malignant cell to PDT. Lasers Surg. Med. 51:301–308, 2019. © 2019 Wiley Periodicals, Inc.

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Terahertz Spectroscopy Sheds Light on Real‐Time Exchange Kinetics Occurring through Plasma Membrane during Photodynamic Therapy Treatment

et al. 2023

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Methods to follow in real time complex processes occurring along living cell membranes such as cell permeabilization are rare. Here, the terahertz spectroscopy reveals early events in plasma membrane alteration generated during photodynamic therapy (PDT) protocol, events which are not observable in any other conventional biological techniques performed in parallel as comparison. Photodynamic process is examined in Madin-Darby canine kidney cells using Pheophorbide (Pheo) photosensitizer alone or alternatively encapsulated in poly(ethylene oxide)-block-poly(𝝐-caprolactone) micelles for drug delivery purpose. Terahertz spectroscopy (THz) reveals that plasma membrane permeabilization starts simultaneously with illumination and is stronger when photosensitizer is encapsulated. In parallel, the exchange of biological species is assessed. Over several hours, this conventional approach demonstrates significant differences between free and encapsulated Pheo, the latter leading to high penetration of propidium iodide, Na + and Ca 2+ ions, and a high level of leakage of K + , ATP, and lactate dehydrogenase. THz spectroscopy provides, in a single measurement, the relative number of defects per membrane surface created after PDT, which is not achieved by any other method, providing early, sensitive real-time information. THz spectroscopy is therefore a promising technique and can be applied to any biological topic requiring the examination of short-term plasma membrane permeabilization.

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Enhanced phototoxicity of photodynamic treatment by Cx26‐composed GJIC via ROS‐, calcium‐ and lipid peroxide‐mediated pathways

Cited by 15 publications

References 33 publications

Biophotonics in China

Biophotonics in China

Decreased phototoxicity of photodynamic therapy by Cx32/Cx26‐composed GJIC: A “Good Samaritan” effect

Terahertz Spectroscopy Sheds Light on Real‐Time Exchange Kinetics Occurring through Plasma Membrane during Photodynamic Therapy Treatment

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