Enhanced poly-γ-glutamic acid synthesis in Corynebacterium glutamicum by reconstituting PgsBCA complex and fermentation optimization

Xu, Guoqiang; Wang, Jiyue; Shen, Jiancheng; Zhu, Yaxin; Liu, Wanjing; Chen, Yuhang; Zha, Jian; Zhang, Xiaomei; Zhang, Xiaojuan; Shi, Jinsong; Koffas, Mattheos A.G.; Xu, Zhenghong

doi:10.1016/j.ymben.2023.12.008

Cited by 7 publications

(2 citation statements)

References 35 publications

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“…This is especially relevant for the integration of P hyspank , used to express the pgs operon genes, which highlighted the strong impact of the pgs operon transcription on γ-PGA production compared with the activated original promoter (by the swrA + degU32 Hy mutation). The overexpression of the pgs operon genes through different promoters produced conflicting results in terms of polymer production performance [28,31,58,59]. The use of different promoters in different strains and the use of different carbon sources is likely to provide non-comparable outcomes.…”

Section: Discussionmentioning

confidence: 99%

“…Previous works indicated that metabolic engineering interventions in different hosts were able to enhance γ-PGA yield and increase the sustainability of polymer production. The expression of a completely heterologous γ-PGA synthesis pathway has been shown to provide polymer production capability to Escherichia coli [27] and Corynebacterium glutamicum [28,29], the latter reaching γ-PGA titers of about 50 g/L, which is a competitive production level, comparable with natural producers. However, most reports describe the genetic optimization of natural γ-PGA producers such as B. subtilis and B. amyloliquefaciens.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic Engineering of Bacillus subtilis for the Production of Poly-γ-Glutamic Acid from Glycerol Feedstock

Pasotti,

Massaiu,

Magni

et al. 2024

Fermentation

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Poly-γ-glutamic acid (γ-PGA) is an attractive biopolymer for medical, agri-food, and environmental applications. Although microbial synthesis by Bacilli fed on waste streams has been widely adopted, the obtainment of efficient sustainable production processes is still under investigation by bioprocess and metabolic engineering approaches. The abundant glycerol-rich waste generated in the biodiesel industry can be used as a carbon source for γ-PGA production. Here, we studied fermentation performance in different engineered Bacillus subtilis strains in glycerol-based media, considering a swrA+ degU32Hy mutant as the initial producer strain and glucose-based media for comparison. Modifications included engineering the biosynthetic pgs operon regulation (replacing its native promoter with Physpank), precursor accumulation (sucCD or odhAB deletion), and enhanced glutamate racemization (racE overexpression), predicted as crucial reactions by genome-scale model simulations. All interventions increased productivity in glucose-based media, with Physpank-pgs ∆sucCD showing the highest γ-PGA titer (52 g/L). Weaker effects were observed in glycerol-based media: ∆sucCD and Physpank-pgs led to slight improvements under low- and high-glutamate conditions, respectively, reaching ~22 g/L γ-PGA (26% increase). No performance decrease was detected by replacing pure glycerol with crude glycerol waste from a biodiesel plant, and by a 30-fold scale-up. These results may be relevant for improving industrial γ-PGA production efficiency and process sustainability using waste feedstock. The performance differences observed between glucose and glycerol media also motivate additional computational and experimental studies to design metabolically optimized strains.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabolic Engineering of Bacillus subtilis for the Production of Poly-γ-Glutamic Acid from Glycerol Feedstock

Pasotti,

Massaiu,

Magni

et al. 2024

Fermentation

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Production of gamma‐polyglutamic acid microgel by Bacillus species: Industrial applications and future perspectives

Pal,

Singh,

Sarangi

et al. 2024

Polymers for Advanced Techs

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Abstractγ‐Polyglutamic acid (γ‐PGA) microgel, produced by Bacillus spp., represents a promising biomaterial with diverse industrial applications due to its biodegradability, biocompatibility, and nontoxic nature. This review explores the current methodologies in the industrial production of γ‐PGA microgel, emphasizing the optimization of fermentation conditions, genetic engineering of Bacillus strains, and advances in downstream processing techniques. Key applications in pharmaceuticals, agriculture, and environmental management are discussed, highlighting its role in drug delivery systems, as a biocontrol agent, and in wastewater treatment. Future perspectives include enhancing production efficiency through synthetic biology, expanding its application scope, and addressing economic and regulatory challenges to facilitate broader adoption. The integration of innovative technologies and multidisciplinary approaches is crucial for the sustainable development and commercial success of γ‐PGA microgel.

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Metabolic engineering of Bacillus licheniformis DW2 for ectoine production

Li,

Dong,

Yang

et al. 2025

World J Microbiol Biotechnol

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Enhanced poly-γ-glutamic acid synthesis in Corynebacterium glutamicum by reconstituting PgsBCA complex and fermentation optimization

Cited by 7 publications

References 35 publications

Metabolic Engineering of Bacillus subtilis for the Production of Poly-γ-Glutamic Acid from Glycerol Feedstock

Metabolic Engineering of Bacillus subtilis for the Production of Poly-γ-Glutamic Acid from Glycerol Feedstock

Production of gamma‐polyglutamic acid microgel by Bacillus species: Industrial applications and future perspectives

Metabolic engineering of Bacillus licheniformis DW2 for ectoine production

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