Enhanced polychlorinated biphenyl removal in a switchgrass rhizosphere by bioaugmentation with Burkholderia xenovorans LB400

Liang, Yi; Meggo, Richard E.; Hu, Dewei; Schnoor, Jerald L.; Mattes, Timothy E.

doi:10.1016/j.ecoleng.2014.07.046

Cited by 52 publications

(33 citation statements)

References 73 publications

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“…This species was tested for PCB degradation in the rhizosphere of Panicum virgatum L. and was found to be responsible for the removal of 47.3 % of the PCB pollutants present (Liang et al 2014). Recently, Burkholderia sp.…”

Section: Bioremediationmentioning

confidence: 99%

To split or not to split: an opinion on dividing the genus Burkholderia

et al. 2015

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The genus Burkholderia is a large group of species of bacteria that inhabit a wide range of environments. We previously recommended, based on multilocus sequence analysis, that the genus be separated into two distinct groups-one that consists predominantly of human, plant, and animal pathogens, including several opportunistic pathogens, and a second, much larger group of species comprising plant-associated beneficial and environmental species that are primarily known not to be pathogenic. This second group of species is found mainly in soils, frequently in association with plants as plant growth-promoting bacteria. They also possess genetic traits that bestow them with an added potential for agriculture and soil restoration, such as nitrogen fixation, phosphate solubilization, iron sequestration, and xenobiotic degradation, and they are not pathogenic. In this review, we present an update of current information on this second group of Burkholderia species, with the goal of focusing attention on their use in agriculture and environmental remediation. We describe their distribution in the environment, their taxonomy and genetic features, and their relationship with plants as either associative nitrogen-fixers or legume-nodulating/nitrogen-fixing bacteria.We also propose that a concerted and coordinated effort be made by researchers on Burkholderia to determine if a definitive taxonomic split of this very large genus is justified, especially now as we describe here for the first time intermediate groups based upon their 16S rRNA sequences. We need to learn more about the plant-associated Burkholderia strains regarding their potential for pathogenicity, especially in those strains intermediate between the two groups, and to discover whether gene exchange occurs between the symbiotic and pathogenic Burkholderia species. The latter studies will require both field and laboratory analyses of gene loss and gain.

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Section: Bioremediationmentioning

confidence: 99%

To split or not to split: an opinion on dividing the genus Burkholderia

et al. 2015

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“…Another approach that bypasses plant genetic engineering hur dles and possible issues with the use of genetically modified plants involves phytore mediation combined with soil bioaugmentation using natural bacterial isolates that degrade the contaminants of interest. Such a study demonstrated that B. xenovorans LB400 could enhance phytoremediation of polychlorinated biphenyls by switch grass [88].…”

Section: Phytoremediation: the Expression Of Bacterial Dioxygenases Imentioning

confidence: 95%

Application of Aromatic Hydrocarbon Dioxygenases

Tan

Parales

2016

Green Biocatalysis

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c h a p t e r 1 7 INTRODUCTIONThe first reported aromatic hydrocarbon dioxygenase, toluene dioxygenase, was identified to catalyze cis-dihydroxylation of benzene and toluene in Pseudomonas putida F1 [1, 2]. Since then, many more dioxygenases have been studied, and the crys tal structures of several have been determined [3]. This group of enzymes plays an important role in the initial step in the biodegradation of both naturally occurring and man-made aromatic compounds, including aromatic acids, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and nitroaromatic, aminoaromatic and halogenated aromatic compounds [4,5]. Dioxygenases are nonheme Rieske-type NAD(P)H-dependent enzymes that intro duce both atoms of molecular oxygen into their substrates. The multicomponent enzyme systems are composed of a catalytic oxygenase component and one-or two-electron trans fer proteins, including a flavoprotein reductase, and in some cases a ferredoxin that medi ates electron transfer from the reductase to the oxygenase component ([3]; Figure 17.1).In general, Rieske dioxygenases are capable of oxidizing a broad range of substrates, well beyond the range of compounds that serves as growth substrates for the host bacterium [4,5,7]. For example, toluene dioxygenase from P. putida F1 facilitates the oxidation of over 200 different substrates [7], and naphthalene dioxygenase from Pseudomonas sp. NCIB 9816-4 has been documented to oxidize over 60 different substrates [8]. In addition, these enzymes catalyze diverse types of reactions, including cisdihydroxylations, O-and N-dealkylations, desaturations, and the formation of chiral sulfoxides from sulfides ([7-9]; Figure 17.2). In many cases the products are chiral com pounds that are high in enantiomeric purity [8,10,11]. For the aforementioned reasons, dioxygenases are attractive biocatalysts for bioremediation and industrial applications. CHALLENGES IN AROMATIC HYDROCARBON DIOXYGENASE APPLICATIONSThe practical use of dioxygenases in industry is generally limited to the use of whole-cell biocatalysts due to several challenges. The catalytic activity of dioxygenases is dependent on reducing equivalents provided by NADH or NADPH, which require regeneration by electron transfer proteins [12,13]. The regeneration process is typically a limiting step

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“…The soil microcosm set-up procedure has been described previously (Liang et al 2014b; Meggo and Schnoor 2013). Briefly, a mixture of PCB 52, PCB 77 and PCB 153 (99% pure) (Accustandard Inc., New Haven, CT) was dissolved in hexane and then applied to the PCB free-soil (Nodaway series, silt loam from Middle Amana, Iowa, USA) at a target concentration of 500 ng g −1 each (wet weight).…”

Section: Methodsmentioning

confidence: 99%

Microbial community analysis of switchgrass planted and unplanted soil microcosms displaying PCB dechlorination

Liang

Meggo

et al. 2015

Appl Microbiol Biotechnol

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Polychlorinated biphenyls (PCBs) pose potential risks to human and environmental health because they are carcinogenic, persistent and bioaccumulative. In this study we investigated bacterial communities in soil microcosms spiked with PCB 52, 77 and 153. Switchgrass (Panicum virgatum) was employed to improve overall PCB removal and redox cycling (i.e. sequential periods of flooding followed by periods of no flooding) was performed in an effort to promote PCB dechlorination. Lesser chlorinated PCB transformation products were detected in all microcosms, indicating the occurrence of PCB dechlorination. Terminal restriction fragment length polymorphism (T-RFLP) and clone library analysis showed that PCB spiking, switchgrass planting and redox cycling affected the microbial community structure. Putative organohalide-respiring Chloroflexi populations, which were not found in unflooded microcosms, were enriched after two weeks of flooding in the redox-cycled microcosms. Sequences classified as Geobacter sp. were detected in all microcosms, and were most abundant in the switchgrass-planted microcosm spiked with PCB congeners. The presence of possible organohalide-respiring bacteria in these soil microcosms suggests they play a role in PCB dechlorination therein.

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Enhanced polychlorinated biphenyl removal in a switchgrass rhizosphere by bioaugmentation with Burkholderia xenovorans LB400

Cited by 52 publications

References 73 publications

To split or not to split: an opinion on dividing the genus Burkholderia

To split or not to split: an opinion on dividing the genus Burkholderia

Application of Aromatic Hydrocarbon Dioxygenases

Microbial community analysis of switchgrass planted and unplanted soil microcosms displaying PCB dechlorination

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