Enhanced pro-inflammatory cytokine production in Gαi2-deficient mice on colitis prone and colitis resistant 129Sv genetic backgrounds

Bjursten, Malin; Hultgren, Olof; Hörnquist, Elisabeth Hultgren

doi:10.1016/j.cellimm.2004.05.001

Cited by 14 publications

(9 citation statements)

References 15 publications

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“…Mice with intestinal inflammation manifesting increased transcript levels of proinflammatory cytokines therefore also demonstrate higher levels of systemic DNA damage to various cell types. Increased blood and stool cytokine levels of proinflammatory cytokines have similarly been reported in IL‐10 –/– mice with colitis,26–28 in Gαi2 –/– mice29–31 and in DSS‐induced colitis in wildtype mice,13, 18 supporting our observations.…”

Section: Resultssupporting

confidence: 89%

Preparation and dynamic mechanical properties of polyurethane‐modified epoxy composites filled with functionalized fly ash particulates

Xiao

2007

J of Applied Polymer Sci

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Fly ash (FA) is a porous ceramic, which has proved to have some favorable functions. In this article, we have prepared a series of fly ash filled composites, where the polyurethane-(PU) modified epoxy (EP) was acted as the matrix. With the purpose of characterizing the effect of the content of PU in the matrices and the surfacetreatment (ST) of fly ash particulates on the dynamic mechanical properties of composites, Fourier transform infrared spectral analysis (FTIR), microstructure observation, impact property test as well as dynamic mechanical analysis (DMA) were systematically investigated. With FTIR test, it can be found that the chemical reactions occur, and new chemical structures are formed between EP and PU, and the functionalized groups existing on the surface of fly ash particulates can also be detected. In fractographs observation of composites, it can be seen that the silane coupling agent improves the interfacial bonding conditions between fly ash particulates and the matrix. Impact property test result reveals that PU improves the toughness of EP. Dynamic mechanical analysis of the composites is carried out from À40 to 1508C using a tension-compression mode. Results show that the composites, whose matrices are with 10 and 20 wt % PU, may possess better dynamic mechanical properties as compared with the other composites.

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Section: Resultssupporting

confidence: 89%

Preparation and dynamic mechanical properties of polyurethane‐modified epoxy composites filled with functionalized fly ash particulates

Xiao

2007

J of Applied Polymer Sci

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“…Subsequent QRT-PCR studies also confirmed that TNFα levels following a LPS challenge were significantly greater in Gαi2-deficient cells. This is consistent with the published data from Gαi2 (−/−) mice [26], [54].…”

Section: Discussionsupporting

confidence: 93%

“…Gαi2 has been reported to inhibit the activities of types I, V, and VI adenylyl cyclase isoforms directly [17]. As Gαi2 appears to be involved in a complex pattern of signaling [17], [18], [19], [20], [21], [22], [23], [24], [25], [26], [27], insight into its function was sought by silencing the Gαi2 transcript. In addition, the Gβ2 subunits released upon activation of the heterotrimeric G protein activate specific effectors, and previous studies have shown it to be the primary beta subunit for certain Gαi signaling pathways in macrophage cells [5], [6].…”

Section: Resultsmentioning

confidence: 99%

“…In a mouse model, in vitro stimulation of splenocytes with formalin-killed Staphylococcus aureus resulted in significantly increased production of IL1β, TNF, and IL12p40 in Gαi2 (−/−) compared to control mice [26]. Mice with targeted deletion of Gαi2 develop an inflammatory bowel disease closely resembling ulcerative colitis, and the IFNγ and IL1β levels were increased in the inflamed colons [53].…”

Section: Discussionmentioning

confidence: 99%

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RNAi Methodologies for the Functional Study of Signaling Molecules

et al. 2009

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RNA interference (RNAi) was investigated with the aim of achieving gene silencing with diverse RNAi platforms that include small interfering RNA (siRNA), short hairpin RNA (shRNA) and antisense oligonucleotides (ASO). Different versions of each system were used to silence the expression of specific subunits of the heterotrimeric signal transducing G-proteins, G alpha i2 and G beta 2, in the RAW 264.7 murine macrophage cell line. The specificity of the different RNA interference (RNAi) platforms was assessed by DNA microarray analysis. Reliable RNAi methodologies against the genes of interest were then developed and applied to functional studies of signaling networks. This study demonstrates a successful knockdown of target genes and shows the potential of RNAi for use in functional studies of signaling molecules.

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“…Gαi2‐deficient C57BL/6 (B6) mice are relatively resistant to colitis, whereas Gαi2‐deficient 129 mice develop IBD earlier and with greater frequency and severity 7. Linkage analysis has identified a locus on mouse chromosome 3 termed Gpdc1 , which confers colitogenic susceptibility in the Gα i 2‐deficient mouse 8.…”

mentioning

confidence: 99%

Alterations in myeloid dendritic cell innate immune responses in the Gαi2-deficient mouse model of colitis

Peña

Thompson-Snipes²,

Calkins

et al. 2009

Inflammatory Bowel Diseases

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The G protein alpha subunit type-2 (Gαi2)-deficient mouse develops inflammatory bowel disease (IBD) with increased severity in mice on a 129SvEv (129) background compared to the C57BL/6 (B6) background. By breeding these strains to homozygosity for the first time, it became possible to study innate immunity, in this animal model, with more precision than ever before. We learned that dendritic cell distribution and function are significantly different in the two strains of mice.

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Enhanced pro-inflammatory cytokine production in Gαi2-deficient mice on colitis prone and colitis resistant 129Sv genetic backgrounds

Cited by 14 publications

References 15 publications

Preparation and dynamic mechanical properties of polyurethane‐modified epoxy composites filled with functionalized fly ash particulates

Preparation and dynamic mechanical properties of polyurethane‐modified epoxy composites filled with functionalized fly ash particulates

RNAi Methodologies for the Functional Study of Signaling Molecules

Alterations in myeloid dendritic cell innate immune responses in the Gαi2-deficient mouse model of colitis

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