Enhanced Production of 2,3-Butanediol from Sugarcane Molasses

Abstract

2,3-Butanediol has been known as a platform green chemical, and the production cost is the key problem for its large-scale production in which the carbon source occupies a major part. Sugarcane molasses is a by-product of sugar industry and considered as a cheap carbon source for biorefinery. In this paper, the fermentation of 2,3-butanediol with sugarcane molasses was studied by reducing the medium ingredients and operation steps. The fermentation medium was optimized by response surface methodology, and 2,3-butanediol production was explored under the deficiency of sterilization, molasses acidification, and organic nitrogen source. Based on these experiments, the fermentation medium with sugarcane molasses as carbon source was simplified to five ingredients, and the steps of molasses acidification and medium sterilization were reduced; thus, the cost was reduced and the production of 2,3-butanediol was enhanced. Under fed-batch fermentation, 99.5 g/L of 2,3-butanediol and acetoin was obtained at 60 h with a yield of 0.39 g/g sugar.

Biochemical conversion of sugarcane straw hemicellulosic hydrolyzate supplemented with co-substrates for xylitol production

“…Furthermore, these results lead to the possibility of studying the utilization of complex sources of sucrose as supplementation of the hemicellulosic hydrolyzate, such as sugarcane molasses. This byproduct of the sugarcane agroindustry is rich in sucrose (25-40% w/v), nitrogenous compounds, inorganic salts and vitamins (Dai et al, 2015). Thus, xylitol bioproduction from SSHH can be coupled to sugarcane agroindustry processes in a biorefinery context, through utilization of the molasses as source of sucrose and nutrients for supplementation of the hemicellulosic hydrolysate.…”

Combined optimization of N‐terminal site‐specific PEGylation of recombinant hirudin using response surface methodology and kinetic analysis

“…Alternatively, statistical methods, including Plackett-Burman (PB) design and response surface methodology (RSM) are simple and effective process optimization approaches, which can not only reduce the experimental numbers, but also analyze the interactions between different variables [13][14][15][16][17][18][19][20]. Traditional method, for example, one-variable-at-a-time, is not suitable for optimizing this process due to so many factors and their interactions between different variables.…”

“…Traditional method, for example, one-variable-at-a-time, is not suitable for optimizing this process due to so many factors and their interactions between different variables. Alternatively, statistical methods, including Plackett-Burman (PB) design and response surface methodology (RSM) are simple and effective process optimization approaches, which can not only reduce the experimental numbers, but also analyze the interactions between different variables [13][14][15][16][17][18][19][20]. Recently the reports concerning the application of RSM for optimizing the protein PEGylation process are very limited [21].…”

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

“…and Paenibacillusp olymyxa,b yu sing different bioresources, such as xylose, sugar cane molasses, glucose, and corn. [42][43][44] Recently,2 ,3-BDO hasa lso found ad irect application in polyester synthesis. [45][46][47] Glycerol has been produced mainly from fossil-based resources since the 1950s, even if its bioproduction has been known for more than ac entury.…”